CAJ | 학술논문

选用工业大麻新品种龙麻一号的下胚轴作为外植体进行组织培养，对无菌苗的获得、植物生长调节剂的选择及浓度等影响工业大麻组织培养的关键因素进行研究，愈伤组织诱导的最适植物生长调节剂组合为1．0mg／L 6－BA 和0．5mg／L NAA，不定芽分化的最适激素组合为1．0mg／L KT 和0．5mg／L NAA，以1／2MS ＋0．05mg／L IBA ＋0．05mg／L NAA 激素组合生根效果较好。初步建立工业大麻高效再生体系，愈伤组织诱导率达到91．67％，不定芽分化率达到76．67％，生根率达到75％。
선용공업대마신품충룡마일호적하배축작위외식체진행조직배양，대무균묘적획득、식물생장조절제적선택급농도등영향공업대마조직배양적관건인소진행연구，유상조직유도적최괄식물생장조절제조합위1．0mg／L 6－BA 화0．5mg／L NAA，불정아분화적최괄격소조합위1．0mg／L KT 화0．5mg／L NAA，이1／2MS ＋0．05mg／L IBA ＋0．05mg／L NAA 격소조합생근효과교호。초보건립공업대마고효재생체계，유상조직유도솔체도91．67％，불정아분화솔체도76．67％，생근솔체도75％。
The Hypocotyls of a new industrial hemp cultivar Long -ma No.1 were used as explants for tissue culture.The paper studied the key factors of industrial hemp tissue culture such as the physio-logical state of aseptic seedlings,the selection and concentration of plant growth regulators and so on. The best combination of plant growth regulators was 1.0 mg/L 6 -BA and 0.5 mg/L NAA for the induc-tion of callus.The best combination of hormones was 1.0 mg/L KT and 0.5 mg/L NAA for the differen-tiation rate of adventitious bud.The best combination was 1 /2 MS +0.05 mg/L IBA +0.05 mg/L NAA for rooting.The efficient regeneration system for industrial hemp was built preliminarily.The induction rate of callus was 91.67%,the differentiation rate of adventitious bud was 76.67%,the induction rate of root was 75%.