CAJ | 학술논문

亚麻基因组测序完成为全基因组水平分析抗病基因提供了前提条件，而 NBS 类抗病基因是植物中最大的抗病基因家族之一。本研究利用生物信息学方法对亚麻全基因组中 NBS类抗病基因进行了鉴定，对 NBS 类抗病蛋白的理化性质、基因结构、保守基序及其系统发生关系进行了分析。结果表明：在亚麻全基因组中共找到137个 NBS 类抗病基因，其中分子量大于100kDa 序列比例在50％以上，等电点值在5～8的范围内比例为70．07％。系统进化树将 NBS 类基因分成7个亚组，且同一亚组内基因的结构和保守基序保持着较高的一致性。本研究的结果将为亚麻 NBS 类抗病基因家族的基因定位以及相关抗病基因克隆等深入研究提供参考。
아마기인조측서완성위전기인조수평분석항병기인제공료전제조건，이 NBS 류항병기인시식물중최대적항병기인가족지일。본연구이용생물신식학방법대아마전기인조중 NBS류항병기인진행료감정，대 NBS 류항병단백적이화성질、기인결구、보수기서급기계통발생관계진행료분석。결과표명：재아마전기인조중공조도137개 NBS 류항병기인，기중분자량대우100kDa 서렬비례재50％이상，등전점치재5～8적범위내비례위70．07％。계통진화수장 NBS 류기인분성7개아조，차동일아조내기인적결구화보수기서보지착교고적일치성。본연구적결과장위아마 NBS 류항병기인가족적기인정위이급상관항병기인극륭등심입연구제공삼고。
The complete of sequencing flax genome is the premise for analyzing resistance genes at the whole genome level,while NBS resistance gene is one of the biggest disease -resistant gene families in plants.The numbers of NBS genes in flax were identified,and the physicochemical property of pro-tein,genetic structure,conserved motif,and phylogenetic relationship were analyzed by bioinformatics method.The result showed a total of 137 NBS resistance genes of flax were identified,among of them the sequence of molecular weight which were greater than 100 kDa were over 50%,and isoelectric point val-ue which had the range of 5 ~8 was 70.07%.The resistance genes of NBS type were divided into seven subgroups by phylogenetic tree,and the genetic structure and conservative motif of genes within the same subgroup had high consistency.The results would provide theoretical basis for further research about the NBS disease -resistant gene families in flax,as well as laying the foundation for NBS gene location and cloning research.