中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2015年
5期
454-459
,共6页
邓哲治%黄海威%吴萌萌%何国永
鄧哲治%黃海威%吳萌萌%何國永
산철치%황해위%오맹맹%하국영
X线照射%放射性脑损伤%微血管%血脑屏障
X線照射%放射性腦損傷%微血管%血腦屏障
X선조사%방사성뇌손상%미혈관%혈뇌병장
X-ray%Radiation brain injury%Micro-vascular%Blood-brain barrier
目的 探讨放射性脑损伤中可能存在的脑微血管损伤及血脑屏障破坏情况.方法 70只8周龄雄性BALB/C小鼠应用随机数字表分为照射组和对照组,其中照射组行X线全脑照射(单次10 Gy剂量).分别在照射后1、7、30、90、180 d取材(每组每个观察点7只小鼠),采用HE染色观察脑组织微血管形态的改变,采用免疫荧光染色检测全脑第Ⅷ因子相关抗原(vWF)及ZO1蛋白的表达变化.结果 照射后1d开始,照射组小鼠即可见微血管有扩张紊乱,血管内皮细胞与基膜分离,但血管壁仍较薄,血管周隙正常;照射后7d出现血管壁增厚、管腔狭窄;照射后30 d管腔狭窄逐渐加重甚至闭塞,部分血管可见血栓形成,血管周隙开始增大,并持续至180d.与对照组比较,照射后各时间点照射组小鼠脑组织vWF、ZO1蛋白表达阳性细胞数明显减少(vWF:1 d:23.17±2.93 vs 15.80±2.39,7 d:21.25±2.33 vs 11.60±2.30,30 d:19.78±2.16vs 8.20±1.64,90 d:17.21±3.31 vs6.00±2.12,180 d:16.98±1.92 vs 3.80±2.59;ZO1:1 d:26.17±3.31 vs 15.40±1.82,7 d:23.20±2.93vs12.00±1.58,30 d:20.88±2.20vs 9.10±2.55,90 d:18.32±1.87 vs 6.20±1.92,180 d:17.50±1.91vs 2.40±1.52),差异均有统计学意义(P＜0.05);且随着时间延长,照射组小鼠脑组织vWF、ZO1蛋白阳性表达细胞数亦逐渐减少,各时间点间比较差异亦均有统计学意义(P＜0.05).结论 X线全脑照射能诱导小鼠脑微血管持续损伤及血脑屏障紧密连接破坏,导致放射性脑损伤的发生发展.
目的 探討放射性腦損傷中可能存在的腦微血管損傷及血腦屏障破壞情況.方法 70隻8週齡雄性BALB/C小鼠應用隨機數字錶分為照射組和對照組,其中照射組行X線全腦照射(單次10 Gy劑量).分彆在照射後1、7、30、90、180 d取材(每組每箇觀察點7隻小鼠),採用HE染色觀察腦組織微血管形態的改變,採用免疫熒光染色檢測全腦第Ⅷ因子相關抗原(vWF)及ZO1蛋白的錶達變化.結果 照射後1d開始,照射組小鼠即可見微血管有擴張紊亂,血管內皮細胞與基膜分離,但血管壁仍較薄,血管週隙正常;照射後7d齣現血管壁增厚、管腔狹窄;照射後30 d管腔狹窄逐漸加重甚至閉塞,部分血管可見血栓形成,血管週隙開始增大,併持續至180d.與對照組比較,照射後各時間點照射組小鼠腦組織vWF、ZO1蛋白錶達暘性細胞數明顯減少(vWF:1 d:23.17±2.93 vs 15.80±2.39,7 d:21.25±2.33 vs 11.60±2.30,30 d:19.78±2.16vs 8.20±1.64,90 d:17.21±3.31 vs6.00±2.12,180 d:16.98±1.92 vs 3.80±2.59;ZO1:1 d:26.17±3.31 vs 15.40±1.82,7 d:23.20±2.93vs12.00±1.58,30 d:20.88±2.20vs 9.10±2.55,90 d:18.32±1.87 vs 6.20±1.92,180 d:17.50±1.91vs 2.40±1.52),差異均有統計學意義(P＜0.05);且隨著時間延長,照射組小鼠腦組織vWF、ZO1蛋白暘性錶達細胞數亦逐漸減少,各時間點間比較差異亦均有統計學意義(P＜0.05).結論 X線全腦照射能誘導小鼠腦微血管持續損傷及血腦屏障緊密連接破壞,導緻放射性腦損傷的髮生髮展.
목적 탐토방사성뇌손상중가능존재적뇌미혈관손상급혈뇌병장파배정황.방법 70지8주령웅성BALB/C소서응용수궤수자표분위조사조화대조조,기중조사조행X선전뇌조사(단차10 Gy제량).분별재조사후1、7、30、90、180 d취재(매조매개관찰점7지소서),채용HE염색관찰뇌조직미혈관형태적개변,채용면역형광염색검측전뇌제Ⅷ인자상관항원(vWF)급ZO1단백적표체변화.결과 조사후1d개시,조사조소서즉가견미혈관유확장문란,혈관내피세포여기막분리,단혈관벽잉교박,혈관주극정상;조사후7d출현혈관벽증후、관강협착;조사후30 d관강협착축점가중심지폐새,부분혈관가견혈전형성,혈관주극개시증대,병지속지180d.여대조조비교,조사후각시간점조사조소서뇌조직vWF、ZO1단백표체양성세포수명현감소(vWF:1 d:23.17±2.93 vs 15.80±2.39,7 d:21.25±2.33 vs 11.60±2.30,30 d:19.78±2.16vs 8.20±1.64,90 d:17.21±3.31 vs6.00±2.12,180 d:16.98±1.92 vs 3.80±2.59;ZO1:1 d:26.17±3.31 vs 15.40±1.82,7 d:23.20±2.93vs12.00±1.58,30 d:20.88±2.20vs 9.10±2.55,90 d:18.32±1.87 vs 6.20±1.92,180 d:17.50±1.91vs 2.40±1.52),차이균유통계학의의(P＜0.05);차수착시간연장,조사조소서뇌조직vWF、ZO1단백양성표체세포수역축점감소,각시간점간비교차이역균유통계학의의(P＜0.05).결론 X선전뇌조사능유도소서뇌미혈관지속손상급혈뇌병장긴밀련접파배,도치방사성뇌손상적발생발전.
Objective To investigate the possible presence of radiation brain injury (RBI) in vascular injury and blood-brain barrier (BBB) damage.Methods Seventy 8 weeks old male BALB/C mice were randomly divided into irradiation group and control group;mice in the two groups received whole-brain exposure with a single dose of 10 Gy irradiation or sham irradiation.Tissue samples were taken 1,7,30,90 and 180 days after irradiation (7 mice in each time point per group);HE staining was used to observe the microvascular morphology and density changes;immune-fluorescence staining was used to visualize the differential expressions of factor Ⅷ related antigen (vWF) and zonulaoccludens-1 (ZO-1).Results Micro-vascular disorders began to appear from the first day of irradiation,deteriorating with time extension gradually.After the X-ray irradiation exposure,the protein expressions of vWF and ZO-1 in the irradiation group were significantly decreased as compared with those in the control group 1,7,30,90 and 180 days after irradiation (vWF:23.17±2.93 vs 15.80±2.39,21.25±2.33 vs 11.60±2.3,19.78±2.16 vs 8.20±1.64,17.21±3.31 vs 6.00±2.12 and 16.98±1.92 vs 3.80±2.59;ZO1:26.17±3.31 vs 15.40±1.82,23.20±2.93 vs 12.00±1.58,20.88±2.20 vs 9.10±2.55,18.32±1.87 vs 6.20±1.92 and 17.50±1.91 vs 2.40±1.52,P＜0.05);besides,the decreased expression ofvWF and ZO-1 in the irradiation group showed a time-depended manner,with significant differences between each two time points (P＜0.05).Conclusion X-rays may induce persistent micro-vascular injury and destroyed BBB tight junctions,which may be closely related to the occurrence and development of RBI.
