CAJ | 학술논문

目的观察丹红注射液对Ⅰ型糖尿病大鼠周围神经病变的作用.方法 40只Wistar雄性大鼠按随机数字表法分为丹红注射液组、甲钴胺组、模型组和正常组,每组10只.前3组采用链脲佐菌素腹腔注射法制备Ⅰ型糖尿病大鼠模型,并于成模后第8周末时分别按实验设计给药.至成模后第12周末时,记录各组大鼠血糖、体质量,并应用足印行走箱行足迹步态分析,应用生理记录仪行神经传导速度检测,应用免疫组化染色及HE染色检测表皮内神经纤维及周围神经组织的病理学改变.结果与模型组相比,丹红注射液组神经传导速度增快[(62.05±5.45) m/s vs(37.72±4.06) m/s],足迹步态参数足印长、足中间三趾宽、足印宽明显改善[(22.00±0.34) mm vs(22.74±0.19) mm;(10.79±0.22) mm vs (11.49±0.14) mm;(17.50±0.16) mm vs (17.67±0.17) mm],差异均有统计学意义(P＜0.05).模型组大鼠表皮内神经纤维染色模糊、稀疏零散、不易辨认,严重者无神经纤维显示,而丹红注射液组神经纤维走形不规则、数量减少、断断续续、稀疏零散,但较模型组明显改善.模型组大鼠神经组织严重水肿,髓鞘缺失,难以辨认,局部有空泡变性,轴索水肿,部分轴索分离、变性,而丹红注射液组神经水肿,髓鞘模糊,轴索肿胀,但较模型组明显改善.结论丹红注射液可以预防和改善Ⅰ型糖尿病大鼠的周围神经病变.
목적 관찰단홍주사액대Ⅰ형당뇨병대서주위신경병변적작용.방법 40지Wistar웅성대서안수궤수자표법분위단홍주사액조、갑고알조、모형조화정상조,매조10지.전3조채용련뇨좌균소복강주사법제비Ⅰ형당뇨병대서모형,병우성모후제8주말시분별안실험설계급약.지성모후제12주말시,기록각조대서혈당、체질량,병응용족인행주상행족적보태분석,응용생리기록의행신경전도속도검측,응용면역조화염색급HE염색검측표피내신경섬유급주위신경조직적병이학개변.결과 여모형조상비,단홍주사액조신경전도속도증쾌[(62.05±5.45) m/s vs(37.72±4.06) m/s],족적보태삼수족인장、족중간삼지관、족인관명현개선[(22.00±0.34) mm vs(22.74±0.19) mm;(10.79±0.22) mm vs (11.49±0.14) mm;(17.50±0.16) mm vs (17.67±0.17) mm],차이균유통계학의의(P＜0.05).모형조대서표피내신경섬유염색모호、희소령산、불역변인,엄중자무신경섬유현시,이단홍주사액조신경섬유주형불규칙、수량감소、단단속속、희소령산,단교모형조명현개선.모형조대서신경조직엄중수종,수초결실,난이변인,국부유공포변성,축색수종,부분축색분리、변성,이단홍주사액조신경수종,수초모호,축색종창,단교모형조명현개선.결론 단홍주사액가이예방화개선Ⅰ형당뇨병대서적주위신경병변.
Objective To investigate the effect of Dan-Hong injection on peripheral neuropathy in experimental rats with type Ⅰ diabetes.Methods Forty Wistar male rats were chose in our study and divided into Dan-Hong injection group,mecobalamin treatment group,model group and normal control group (n=10);rat models of type Ⅰ diabetes in the first three groups were induced by intraperitoneal injection of streptozotocin.Different drugs were given to these rats,and 12 weeks after model making,the blood glucose level and the body weight were measured;footprints gaits were analyzed;nerve conduction velocity was observed;immumohistochemical staining and HE staining were employed to detect the pathological alterations of intraepidermal nerve fiber and peripheral nerve tissues.Results As compared with model group,rats in Dan-Hong injection group had increased nerve conduction velocity and footprint parameters with statistical differences ([62.05±5.45] m/s vs [37.72±4.06] m/s;[22.00±0.34] mm vs [22.74±0.19] mm;[10.79±0.22] mm vs [11.49±0.14] mm;[17.50±0.16] mm vs [17.67±0.17] mm) (P＜0.05).The number of intraepidermal nerve fiber was increased and the pathological damage of peripheral nerves was obvious improved in the Dan-Hong injection group as compared with those in the model group;the nervous tissues showed edema with myelin fuzzy and swelling of the axon,which had been much improved as compared with those in the model group.Conclusions Dan-Hong injection can prevent and improve peripheral neuropathy of rats with type Ⅰ diabetes.