CAJ | 학술논문

目的探讨腹腔注射安钠咖对大鼠学习记忆能力及海马神经元形态的影响.方法 36只雄性SD大鼠按随机数字表法分为3组:对照组、安钠咖小剂量组及安钠咖大剂量组,每组12只,分别每天给药1次(腹腔注射l mL生理盐水或60 mg/kg安钠咖或120 mg/kg安钠咖),连续20d.末次注射后次日应用Morris水迷宫实验(定位航行试验、空间探索试验、可见平台试验)评定各组大鼠的空间学习记忆能力,行为学实验结束后大鼠断头取脑、HE染色观察海马神经元形态的改变.结果在定位航行试验中,安钠咖大剂量组各训练日的平均逃避潜伏期与对照组及安钠咖小剂量组相比差异均有统计学意义(P＜0.05),而安钠咖小剂量组各训练日的平均逃避潜伏期与对照组比较差异均无统计学意义(P＞0.05);在空间探索试验中,3组大鼠在目标象限内穿越原平台次数平均值比较差异无统计学意义(P＞0.05).HE染色观察发现,安钠咖小剂量组大鼠除海马CA1区神经元细胞排列紊乱、稀疏外,其他部位与对照组相比无明显变化;而安钠咖大剂量组大鼠海马CA1区和齿状回区神经元细胞排列紊乱、核固缩深染、胞浆浓缩.结论大剂量安钠咖可使大鼠学习能力下降,且会损害海马神经元.
목적 탐토복강주사안납가대대서학습기억능력급해마신경원형태적영향.방법 36지웅성SD대서안수궤수자표법분위3조:대조조、안납가소제량조급안납가대제량조,매조12지,분별매천급약1차(복강주사l mL생리염수혹60 mg/kg안납가혹120 mg/kg안납가),련속20d.말차주사후차일응용Morris수미궁실험(정위항행시험、공간탐색시험、가견평태시험)평정각조대서적공간학습기억능력,행위학실험결속후대서단두취뇌、HE염색관찰해마신경원형태적개변.결과 재정위항행시험중,안납가대제량조각훈련일적평균도피잠복기여대조조급안납가소제량조상비차이균유통계학의의(P＜0.05),이안납가소제량조각훈련일적평균도피잠복기여대조조비교차이균무통계학의의(P＞0.05);재공간탐색시험중,3조대서재목표상한내천월원평태차수평균치비교차이무통계학의의(P＞0.05).HE염색관찰발현,안납가소제량조대서제해마CA1구신경원세포배렬문란、희소외,기타부위여대조조상비무명현변화;이안납가대제량조대서해마CA1구화치상회구신경원세포배렬문란、핵고축심염、포장농축.결론 대제량안납가가사대서학습능력하강,차회손해해마신경원.
Objective To observe the effect ofintraperitoneal injection of sodium benzoate on learning and memory abilities and morphology of hippocampal neurons in rats.Methods Thirty-six male SD rats were randomly divided into three groups:control group,sodium benzoate low-dose group and sodium benzoate high-dose group (n=12);intraperitoneal injection of 1 mL normal saline,60 mg/kg sodium benzoate or 120 mg/kg sodium benzoate was performed on the rats of three groups,respectively,for 20 d.Morris water maze test (place navigation,space exploration and visible platform searching) was used to assess the spatial learning and memory of rats,and HE staining was used to observe morphological changes in hippocampal neurons.Results The escape latency of rats in the sodium benzoate high-dose group was statistically significant in the navigation test as compared with that in the control group and the sodium benzoate low-dose group (P＜0.05);no significant difference was noted between sodium benzoate low-dose group and control group (P＞0.05).In probe test,no significant differences in the number crossing target quadrant were found after withdrawal of platform between groups (P＞0.05).HE staining showed that hippocampal CA1 neurons were sparse,showing a disordered arrangement in sodium benzoate low-dose group;the hippocampal neurons of CA1 region and fascia dentata region in sodium benzoate high dose group showed messed arrangement,nuclear pyknosis and cytoplasmic concentration as compared with that in the control group.Conclusion The high-dose sodium benzoate can decrease the learning ability of rats and impair hippocampal neurons.