中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2015年
5期
469-472
,共4页
张秋生%张猛%黄贤键%刘晓佳%李维平
張鞦生%張猛%黃賢鍵%劉曉佳%李維平
장추생%장맹%황현건%류효가%리유평
颅脑损伤%氧化应激%细胞骨架蛋白%羰基化
顱腦損傷%氧化應激%細胞骨架蛋白%羰基化
로뇌손상%양화응격%세포골가단백%탄기화
Traumatic brain injury%Oxidative stress%Cytoskeletal protein%Carbonylation
目的 研究不同程度颅脑损伤(TBI)后脑组织氧化应激及细胞骨架蛋白羰基化水平变化及意义.方法 SD成年大鼠15只按随机数字表法分为轻度TBI组(n=5)、重度TBI组(n=5)和假手术组(n=5),应用液压颅脑损伤仪根据相应参数制备轻度、重度及假手术组动物模型.应用酶联免疫吸附法和Western blotting检测TBI后24 h大鼠脑组织中还原型谷胱甘肽(GSH)、丙二醛(MDA)及细胞骨架蛋白[β-肌动蛋白、β-微管蛋白和胶质纤维酸性蛋白(GFAP)]羰基化水平,并应用Western blotting检测轴索损伤标志物磷酸化tau(p-tau)蛋白水平.结果 轻度、重度TBI组大鼠脑组织中MDA水平分别为(389.62±29.95) μmol/g、(642.50±37.56) μmol/g,较假手术组[(233.94±25.08) μmol/g]显著升高,差异均有统计学意义(P＜0.05);GSH水平分别为(352.10±37.75) μmol/g、(153.27±43.49) μmol/g,较假手术组[(492.48±41.43) μmol/g]显著降低,差异均有统计学意义(P＜0.05);β-肌动蛋白、β-微管蛋白和GFAP在轻度TBI组羰基化比例分别为0.099±0.104、0.194±0.114、0.643±0.037,重度TBI组分别为0.142±0.017、0.290±0.029、0.902±0.021,较假手术组(0.068±0.017、0.108±0.016、0.673±0.032)明显升高,差异均有统计学意义(P＜ 0.05);p-tau比例在轻度、重度TBI组分别为0.289±0.014、0.373±0.012,较假手术组(0.185±0.009)差异均有统计学意义(P＜0.05).结论 TBI后脑组织氧化应激及细胞骨架蛋白羰基化水平随着TBI程度的增加而增加,可造成神经轴索运输障碍而导致轴索损伤加重.
目的 研究不同程度顱腦損傷(TBI)後腦組織氧化應激及細胞骨架蛋白羰基化水平變化及意義.方法 SD成年大鼠15隻按隨機數字錶法分為輕度TBI組(n=5)、重度TBI組(n=5)和假手術組(n=5),應用液壓顱腦損傷儀根據相應參數製備輕度、重度及假手術組動物模型.應用酶聯免疫吸附法和Western blotting檢測TBI後24 h大鼠腦組織中還原型穀胱甘肽(GSH)、丙二醛(MDA)及細胞骨架蛋白[β-肌動蛋白、β-微管蛋白和膠質纖維痠性蛋白(GFAP)]羰基化水平,併應用Western blotting檢測軸索損傷標誌物燐痠化tau(p-tau)蛋白水平.結果 輕度、重度TBI組大鼠腦組織中MDA水平分彆為(389.62±29.95) μmol/g、(642.50±37.56) μmol/g,較假手術組[(233.94±25.08) μmol/g]顯著升高,差異均有統計學意義(P＜0.05);GSH水平分彆為(352.10±37.75) μmol/g、(153.27±43.49) μmol/g,較假手術組[(492.48±41.43) μmol/g]顯著降低,差異均有統計學意義(P＜0.05);β-肌動蛋白、β-微管蛋白和GFAP在輕度TBI組羰基化比例分彆為0.099±0.104、0.194±0.114、0.643±0.037,重度TBI組分彆為0.142±0.017、0.290±0.029、0.902±0.021,較假手術組(0.068±0.017、0.108±0.016、0.673±0.032)明顯升高,差異均有統計學意義(P＜ 0.05);p-tau比例在輕度、重度TBI組分彆為0.289±0.014、0.373±0.012,較假手術組(0.185±0.009)差異均有統計學意義(P＜0.05).結論 TBI後腦組織氧化應激及細胞骨架蛋白羰基化水平隨著TBI程度的增加而增加,可造成神經軸索運輸障礙而導緻軸索損傷加重.
목적 연구불동정도로뇌손상(TBI)후뇌조직양화응격급세포골가단백탄기화수평변화급의의.방법 SD성년대서15지안수궤수자표법분위경도TBI조(n=5)、중도TBI조(n=5)화가수술조(n=5),응용액압로뇌손상의근거상응삼수제비경도、중도급가수술조동물모형.응용매련면역흡부법화Western blotting검측TBI후24 h대서뇌조직중환원형곡광감태(GSH)、병이철(MDA)급세포골가단백[β-기동단백、β-미관단백화효질섬유산성단백(GFAP)]탄기화수평,병응용Western blotting검측축색손상표지물린산화tau(p-tau)단백수평.결과 경도、중도TBI조대서뇌조직중MDA수평분별위(389.62±29.95) μmol/g、(642.50±37.56) μmol/g,교가수술조[(233.94±25.08) μmol/g]현저승고,차이균유통계학의의(P＜0.05);GSH수평분별위(352.10±37.75) μmol/g、(153.27±43.49) μmol/g,교가수술조[(492.48±41.43) μmol/g]현저강저,차이균유통계학의의(P＜0.05);β-기동단백、β-미관단백화GFAP재경도TBI조탄기화비례분별위0.099±0.104、0.194±0.114、0.643±0.037,중도TBI조분별위0.142±0.017、0.290±0.029、0.902±0.021,교가수술조(0.068±0.017、0.108±0.016、0.673±0.032)명현승고,차이균유통계학의의(P＜ 0.05);p-tau비례재경도、중도TBI조분별위0.289±0.014、0.373±0.012,교가수술조(0.185±0.009)차이균유통계학의의(P＜0.05).결론 TBI후뇌조직양화응격급세포골가단백탄기화수평수착TBI정도적증가이증가,가조성신경축색운수장애이도치축색손상가중.
Objective To investigate the oxidative stress and cytoskeleton protein carbonylation in rat brains after different severities of traumatic brain injury (TBI).Methods Fluid percussion percussion device was used to establish the mild,severe and sham-operated Sprague-Dawley rat models (n=15);24 h after that,enzyme linked immunosorbent assay was used to detect the levels of malondialdehyde (MDA) and glutathione (GSH),and Western blotting was employed to detect the cytoskeletal proteins (β-actin,β-tublin and glial fibrillary acidic [GFAP]) carbonylation levels;phosphorylated tau (p-tau) protein expressions were examined by Western blotting.Results The expression levels of MDA in mild TBI group and severe TBI group were (389.62±29.95) μmol/g and (642.50±37.56) μmol/g,respectively,which was significantly increased as compared with the MDA level ([233.94±25.08] μmol/g) in sham-operated group (P＜0.05).The expression level of GSH in mild TBI group and severe TBI group was (352.10±37.75) μmol/g and (153.27 ±43.49) μmol/g,respectively,which was significantly decreased as compared with the GSH level ([492.48 ±41.43] μmol/g) in sham-operated group (P＜0.05).The β-actin,β-tublin and GFAP proteincarbonylation levels (0.099± 0.104,0.194±0.114 and 0.643±0.037;0.142±0.017,0.290±0.029 and 0.902±0.021) and p-tau level (0.289±0.014 and 0.373±0.012) in mild TBI group and severe TBI group were significantly higher than those in sham-operated group (0.068±0.017,0.108±0.016 and 0.673±0.032;0.185±0.009;P＜0.05).Conclusions The oxidative stress and carbonylation of cytoskeleton proteins are significantly increased after TBI,and the expression levels are correlated with the severity of TBI.The carbonylation of cytoskeleton protein aggravates the axonal injury after TBI.
