CAJ | 학술논문

目的探讨盐霉素对大鼠9L 胶质肉瘤细胞系的体内、外生长和增殖的抑制作用。方法将盐霉素加入体外培养的大鼠9L 胶质肉瘤细胞系中检测其对细胞生长、增殖活动的影响。对40只 Fisher 344大鼠行颅内9L 细胞注射，以制备载瘤大鼠模型。将盐霉素与可生物降解的药物基质聚酸酐类聚合物对羧基苯氧丙烷－癸二酸共聚物（pCPP∶SA）溶解并真空干燥后塑性，制成圆形药物聚合物颗粒。对载瘤大鼠行开颅手术，于瘤腔内植入聚合物颗粒，以观察盐霉素聚合物体内抗肿瘤效果，记录大鼠存活时间，进行进一步生存分析。结果盐霉素对9L 细胞具有明显抑制作用，并表现出剂量依赖性特点。盐霉素聚合物颗粒具有良好生物相容性，能够短时间内稳定释放盐霉素进入局部组织。接受盐霉素聚合物治疗的大鼠，其中位生存期较对照组显著增加；采用30％盐霉素比例聚合物能够在延长大鼠生存时间的同时，降低盐霉素的使用剂量。结论盐霉素对9L 胶质肉瘤细胞具有强大的抑制杀伤作用，采用盐霉素聚合物肿瘤局部给药的方式具有重大临床潜在研究和应用价值。
목적탐토염매소대대서9L 효질육류세포계적체내、외생장화증식적억제작용。방법장염매소가입체외배양적대서9L 효질육류세포계중검측기대세포생장、증식활동적영향。대40지 Fisher 344대서행로내9L 세포주사，이제비재류대서모형。장염매소여가생물강해적약물기질취산항류취합물대최기분양병완－계이산공취물（pCPP∶SA）용해병진공간조후소성，제성원형약물취합물과립。대재류대서행개로수술，우류강내식입취합물과립，이관찰염매소취합물체내항종류효과，기록대서존활시간，진행진일보생존분석。결과염매소대9L 세포구유명현억제작용，병표현출제량의뢰성특점。염매소취합물과립구유량호생물상용성，능구단시간내은정석방염매소진입국부조직。접수염매소취합물치료적대서，기중위생존기교대조조현저증가；채용30％염매소비례취합물능구재연장대서생존시간적동시，강저염매소적사용제량。결론염매소대9L 효질육류세포구유강대적억제살상작용，채용염매소취합물종류국부급약적방식구유중대림상잠재연구화응용개치。
Objective To investigate the inhibition effect of salinomycin polymers on the growth and prolifer-ation of 9L gliosarcoma cells in vitro and in vivo.Methods Salinomycin was applied to the cultured 9L cell lines, and the growth and proliferation ability were tested 40 Fisher 344 rats were received intracranial injection of 9L cells. Polyanhydride polymers were composed with 1,3 -bis (p -carboxyphenoxy)propane (CPP)and sebacic acid (pCPP∶SA),and contained salinomycin.The polymers were manufactured by mix -melt method and shaped into round pills.The polymer complexes were implanted into the tumor cavitiesin vivo on the 5th day after tumor cell injec-tion.The rat survival time and histological characteristics were examined to compare the difference treatment condition of salinomycin polymers.Results Salinomycin could inhibit the growth and proliferation of 9L cells and showed a dose-dependent characteristic.The salinomycin polymers were biodegradable and could release salinomycin into the adja-cent tissue in a short time.Compared with the control group,the median survival time was increased significantly in sali-nomycin polymers group.The 30% salinomycin polymer could increased the median survival time of rats,as well as de-creased the dosage of salinomycin.Conclusion Salinomycin is an effective antitumor drug for the 9L gilosarcoma treatment.Local delivery of salinomycin polymer may be a promising method for the current clinical tumor treatment.