CAJ | 학술논문

配体与受体间的识别及相互作用是分子发挥其功能的重要途径，具有重要的生物学意义。但是，寻找某一分子的受体并不容易，往往成为研究的瓶颈所在。目前寻找及验证某一对配体／受体分子及其对应关系的方法有很多，包括免疫共沉淀、噬菌体展示、酵母双杂交、分子模拟、谷胱甘肽巯基转移酶pull－down、串联亲和纯化等。该文对这些方法的基本原理、简要步骤及优缺点进行综述，为相关研究提供依据。
배체여수체간적식별급상호작용시분자발휘기공능적중요도경，구유중요적생물학의의。단시，심조모일분자적수체병불용역，왕왕성위연구적병경소재。목전심조급험증모일대배체／수체분자급기대응관계적방법유흔다，포괄면역공침정、서균체전시、효모쌍잡교、분자모의、곡광감태구기전이매pull－down、천련친화순화등。해문대저사방법적기본원리、간요보취급우결점진행종술，위상관연구제공의거。
Recognization and interaction between receptor and ligand is an important way for molecules to perform their functions,and has great biological significance.Researches on receptor and ligand have an essential role in biological science.The progress of numerous researches,however,are frequently prevented by the undiscovered receptors of some specified molecular.The methods to find receptor of a molecular include co-immunoprecipitation, phage display, yeast two-hybrid system, molecular docking simulation, glutathione S-transferase pull-down,and tandem affinity purification.Here is to introduce the fundamental theory,proce-dure,merits and demerits of these methods,in order to provide reference for the related research.