中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
6期
1054-1056
,共3页
范蕾%刘敏%吴查青%王伟影%陈卫平
範蕾%劉敏%吳查青%王偉影%陳衛平
범뢰%류민%오사청%왕위영%진위평
四季感冒胶囊%橙皮苷%连翘苷%高效液相色谱法
四季感冒膠囊%橙皮苷%連翹苷%高效液相色譜法
사계감모효낭%등피감%련교감%고효액상색보법
Siji Ganmao capsules%Hesperidin%Phillyrin%HPLC
目的：：建立四季感冒胶囊中橙皮苷和连翘苷的含量测定方法。方法：应用高效液相色谱法测定,色谱柱为Agilent-XDB C18(250 mm ×4.6 mm,5μm),流动相为甲醇-0.1%磷酸,梯度洗脱,流速为1.0 ml·min-1,柱温为30℃,检测波长为277 nm,进样量为5μl。结果：橙皮苷和连翘苷的线性范围分别为12.000~60.000μg·ml-1(r=0.9996)、18.000~90.000μg· ml-1(r=0.9993)；平均加样回收率分别为96.61%(RSD=1.40%),97.66%(RSD=1.27%)。结论：该方法简便易行、准确、重复性好,可用于四季感冒胶囊的含量控制。
目的：：建立四季感冒膠囊中橙皮苷和連翹苷的含量測定方法。方法：應用高效液相色譜法測定,色譜柱為Agilent-XDB C18(250 mm ×4.6 mm,5μm),流動相為甲醇-0.1%燐痠,梯度洗脫,流速為1.0 ml·min-1,柱溫為30℃,檢測波長為277 nm,進樣量為5μl。結果：橙皮苷和連翹苷的線性範圍分彆為12.000~60.000μg·ml-1(r=0.9996)、18.000~90.000μg· ml-1(r=0.9993)；平均加樣迴收率分彆為96.61%(RSD=1.40%),97.66%(RSD=1.27%)。結論：該方法簡便易行、準確、重複性好,可用于四季感冒膠囊的含量控製。
목적：：건립사계감모효낭중등피감화련교감적함량측정방법。방법：응용고효액상색보법측정,색보주위Agilent-XDB C18(250 mm ×4.6 mm,5μm),류동상위갑순-0.1%린산,제도세탈,류속위1.0 ml·min-1,주온위30℃,검측파장위277 nm,진양량위5μl。결과：등피감화련교감적선성범위분별위12.000~60.000μg·ml-1(r=0.9996)、18.000~90.000μg· ml-1(r=0.9993)；평균가양회수솔분별위96.61%(RSD=1.40%),97.66%(RSD=1.27%)。결론：해방법간편역행、준학、중복성호,가용우사계감모효낭적함량공제。
Objective:To establish an HPLC method for the simultaneous determination of hesperidin and phillyrin in Siji Gamao capsules. Methods:HPLC was applied with the chromatographic conditions as follows:the chromatographic column was Agilent-XDB C18 (250 mm × 4. 6 mm,5μm) at 30℃, the mobile phase was methanol-0. 1% phosphoric acid solution with gradient elution, the flow rate was 1. 0 ml·min-1 , the detection wavelength was 277nm and the injection volume was 5μl. Results:The linear range of hesperi-din and phillyrin was 12.000-60.000 μg·ml-1(r=0.999 6) and 18.000-90.000 μg·ml-1(r=0.999 3),respectively; the average recovery was 96.61%(RSD=1.40%) and 97.66%(RSD=1.27%), respectively. Conclusion: The method is simple, accurate and repeatable, which can be used in the quality control of Siji Ganmao capsules.
