中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
6期
1027-1030
,共4页
程东岩%王隶书%程东红%赵大庆%刘永宏
程東巖%王隸書%程東紅%趙大慶%劉永宏
정동암%왕대서%정동홍%조대경%류영굉
赤丹肝纤颗粒%薄层色谱%高效液相色谱法%芍药苷
赤丹肝纖顆粒%薄層色譜%高效液相色譜法%芍藥苷
적단간섬과립%박층색보%고효액상색보법%작약감
Chidan Ganxian granules%TLC%HPLC%Paeoniflorin
目的：：建立赤丹肝纤颗粒的质量标准。方法：采用薄层色谱法对处方中赤芍及虎杖、丹参、三白草、大黄5味中药进行定性鉴别,采用HPLC法测定赤芍中芍药苷的含量。以Apollo C18柱(150 mm ×4.6 mm,5μm)为色谱柱,流动相为乙腈-水(12.5∶87.5),流速为1.0 ml·min-1,检测波长为230 nm,柱温30℃。结果：各TLC色谱中,供试品与各自对照品及对照药材色谱相同的位置上显相同颜色的斑点,且阴性对照无干扰。 HPLC法测定芍药苷在0.120~1.436μg范围内线性关系良好( r=0.9994),平均加样回收率为99.8%,RSD=1.80%(n=6)。结论：本方法简便、准确、重复性好,适用于赤丹肝纤颗粒的质量控制。
目的：：建立赤丹肝纖顆粒的質量標準。方法：採用薄層色譜法對處方中赤芍及虎杖、丹參、三白草、大黃5味中藥進行定性鑒彆,採用HPLC法測定赤芍中芍藥苷的含量。以Apollo C18柱(150 mm ×4.6 mm,5μm)為色譜柱,流動相為乙腈-水(12.5∶87.5),流速為1.0 ml·min-1,檢測波長為230 nm,柱溫30℃。結果：各TLC色譜中,供試品與各自對照品及對照藥材色譜相同的位置上顯相同顏色的斑點,且陰性對照無榦擾。 HPLC法測定芍藥苷在0.120~1.436μg範圍內線性關繫良好( r=0.9994),平均加樣迴收率為99.8%,RSD=1.80%(n=6)。結論：本方法簡便、準確、重複性好,適用于赤丹肝纖顆粒的質量控製。
목적：：건립적단간섬과립적질량표준。방법：채용박층색보법대처방중적작급호장、단삼、삼백초、대황5미중약진행정성감별,채용HPLC법측정적작중작약감적함량。이Apollo C18주(150 mm ×4.6 mm,5μm)위색보주,류동상위을정-수(12.5∶87.5),류속위1.0 ml·min-1,검측파장위230 nm,주온30℃。결과：각TLC색보중,공시품여각자대조품급대조약재색보상동적위치상현상동안색적반점,차음성대조무간우。 HPLC법측정작약감재0.120~1.436μg범위내선성관계량호( r=0.9994),평균가양회수솔위99.8%,RSD=1.80%(n=6)。결론：본방법간편、준학、중복성호,괄용우적단간섬과립적질량공제。
Objective:To establish the quality standard for Chidan Ganxian granules. Methods:TLC was used to identify Paeoni-ae Radix Rubra, Rhizoma Polygoni Cuspidati, Radix Salviae Miltiorrhizae, Herba Saururi and Radix et Rhizoma Rhei in Chidan Ganx-ian granules, and the content of paeoniflorin was determined by HPLC. The stationary phase was an Apollo C18 column ( 150 mm × 4. 6 mm, 5 μm), the mobile phase was acetonitrile-water(12. 5∶87. 5), the flow rate was 1. 0 ml·min-1, the detection wavelength was at 230 nm, and the temperature was 30℃. Results:The characteristic spots of the granules were the same as those of the standard samples without any interference from the negative control. Paeoniflorin had a good linear relationship within the range of 0. 120-1. 436 μg(r=0. 999 4), and the average recovery was 99. 8% with RSD of 1. 80%(n=6). Conclusion:The method is simple, ac-curate, reliable and reproducible, which can be used in the quality control of Chidan Ganxian granules.
