中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
6期
946-948
,共3页
阳春砂%荧光定量PCR%鉴定
暘春砂%熒光定量PCR%鑒定
양춘사%형광정량PCR%감정
Amomum viosum%Fluorescent quantitative PCR%Identify
目的：：分析研究阳春砂基因序列特征，建立基于DNA分子的快速甄别阳春砂的荧光定量PCR技术。方法：收集阳春砂及同科其他样品，经专家鉴别后，提取DNA。根据阳春砂ITS序列两端相对保守区设计引物和探针，优化反应条件，建立阳春砂的荧光定量PCR检测方案。结果：基于阳春砂ITS序列两端相对保守区设计了引物和探针，通过条件优化，建立的荧光定量PCR检测方法能成功对阳春砂样品进行检出，而同科其他非阳春砂样品无扩增曲线。结论：阳春砂药材能够通过除传统专家鉴定方法以外的荧光定量PCR方法快速检出。
目的：：分析研究暘春砂基因序列特徵，建立基于DNA分子的快速甄彆暘春砂的熒光定量PCR技術。方法：收集暘春砂及同科其他樣品，經專傢鑒彆後，提取DNA。根據暘春砂ITS序列兩耑相對保守區設計引物和探針，優化反應條件，建立暘春砂的熒光定量PCR檢測方案。結果：基于暘春砂ITS序列兩耑相對保守區設計瞭引物和探針，通過條件優化，建立的熒光定量PCR檢測方法能成功對暘春砂樣品進行檢齣，而同科其他非暘春砂樣品無擴增麯線。結論：暘春砂藥材能夠通過除傳統專傢鑒定方法以外的熒光定量PCR方法快速檢齣。
목적：：분석연구양춘사기인서렬특정，건립기우DNA분자적쾌속견별양춘사적형광정량PCR기술。방법：수집양춘사급동과기타양품，경전가감별후，제취DNA。근거양춘사ITS서렬량단상대보수구설계인물화탐침，우화반응조건，건립양춘사적형광정량PCR검측방안。결과：기우양춘사ITS서렬량단상대보수구설계료인물화탐침，통과조건우화，건립적형광정량PCR검측방법능성공대양춘사양품진행검출，이동과기타비양춘사양품무확증곡선。결론：양춘사약재능구통과제전통전가감정방법이외적형광정량PCR방법쾌속검출。
Objective:To analyze the genetic sequence characteristics of amomum viosum and establish a rapid identification meth-od for amomum viosum by fluorescent quantitative PCR based on DNA analysis. Methods:Amomum viosum and the other samples be-longing to the same genera were collected and identified by experts in the domain. DNA was isolated using commercial kits. The prim-ers and probe were designed according to the conserved region of ITS in amomum viosum. The reaction conditions were optimized to es-tablish the fluorescent quantitative PCR method for the rapid detection of amomum viosum. Results:The fluorescent quantitative PCR method for the rapid detection of amomum viosum was set up. The method could identify amomum viosum successfully, while those samples in the same genera were without amplification curves. Conclusion: Amomum viosum can be identified rapidly by fluorescent quantitative PCR besides the traditional identification by experts.
