中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
6期
949-951,969
,共4页
龚明%黄杨志%唐海明%贺莲
龔明%黃楊誌%唐海明%賀蓮
공명%황양지%당해명%하련
高效液相色谱法%纤花线纹香茶菜%新西兰牡荆苷2%异夏佛塔苷%夏佛塔苷
高效液相色譜法%纖花線紋香茶菜%新西蘭牡荊苷2%異夏彿塔苷%夏彿塔苷
고효액상색보법%섬화선문향다채%신서란모형감2%이하불탑감%하불탑감
HPLC%Isodon lophanthoides%ViceninⅡ%Isoschaftoside%Schaftoside
目的：：建立同时测定纤花线纹香茶菜中新西兰牡荆苷2、异夏佛塔苷、夏佛塔苷含量的方法,并分析其含量动态变化。方法：采用Phenomenex luna C18(250 mm ×4.6 mm,5μm)色谱柱,流动相为甲醇-0.5%甲酸水溶液,梯度洗脱,流速为0.8 ml·min-1,检测波长为330 nm,柱温为室温(25℃),进样量：10μl。结果：新西兰牡荆苷2、异夏佛塔苷、夏佛塔苷线性范围分别为0.130~3.110μg(r=0.9998)、0.180~4.540μg(r=0.9999)、0.08~1.97μg(r=0.9999)；平均回收率分别为96.8%、99.2%、97.1%,RSD分别为1.9%、1.6%、1.6%(n=6)。上述3种水溶性黄酮成分从幼苗期开始显著累积,异夏佛塔苷和夏佛塔苷在3月和8月达到最高值；新西兰牡荆苷2的含量在1~4月趋于稳定,5月后开始逐渐累积,8月达最高值,之后开始呈逐渐下降的趋势。结论：以水溶性黄酮成分含量为指标,纤花线纹香茶菜的最佳采收期为3月和8月,且以8月份采收的药材质量最佳。
目的：：建立同時測定纖花線紋香茶菜中新西蘭牡荊苷2、異夏彿塔苷、夏彿塔苷含量的方法,併分析其含量動態變化。方法：採用Phenomenex luna C18(250 mm ×4.6 mm,5μm)色譜柱,流動相為甲醇-0.5%甲痠水溶液,梯度洗脫,流速為0.8 ml·min-1,檢測波長為330 nm,柱溫為室溫(25℃),進樣量：10μl。結果：新西蘭牡荊苷2、異夏彿塔苷、夏彿塔苷線性範圍分彆為0.130~3.110μg(r=0.9998)、0.180~4.540μg(r=0.9999)、0.08~1.97μg(r=0.9999)；平均迴收率分彆為96.8%、99.2%、97.1%,RSD分彆為1.9%、1.6%、1.6%(n=6)。上述3種水溶性黃酮成分從幼苗期開始顯著纍積,異夏彿塔苷和夏彿塔苷在3月和8月達到最高值；新西蘭牡荊苷2的含量在1~4月趨于穩定,5月後開始逐漸纍積,8月達最高值,之後開始呈逐漸下降的趨勢。結論：以水溶性黃酮成分含量為指標,纖花線紋香茶菜的最佳採收期為3月和8月,且以8月份採收的藥材質量最佳。
목적：：건립동시측정섬화선문향다채중신서란모형감2、이하불탑감、하불탑감함량적방법,병분석기함량동태변화。방법：채용Phenomenex luna C18(250 mm ×4.6 mm,5μm)색보주,류동상위갑순-0.5%갑산수용액,제도세탈,류속위0.8 ml·min-1,검측파장위330 nm,주온위실온(25℃),진양량：10μl。결과：신서란모형감2、이하불탑감、하불탑감선성범위분별위0.130~3.110μg(r=0.9998)、0.180~4.540μg(r=0.9999)、0.08~1.97μg(r=0.9999)；평균회수솔분별위96.8%、99.2%、97.1%,RSD분별위1.9%、1.6%、1.6%(n=6)。상술3충수용성황동성분종유묘기개시현저루적,이하불탑감화하불탑감재3월화8월체도최고치；신서란모형감2적함량재1~4월추우은정,5월후개시축점루적,8월체최고치,지후개시정축점하강적추세。결론：이수용성황동성분함량위지표,섬화선문향다채적최가채수기위3월화8월,차이8월빈채수적약재질량최가。
Objective:To establish an HPLC method for the simultaneous determination of viceninⅡ, isoschaftoside and schafto-side in Isodon lophanthoides, and analyze their content dynamic changes. Methods:The HPLC system consisted of a Phenomenex luna C18(250 mm ×4.6 mm, 5 μm)column and a solution system of methanol and 0.5% formic acid with gradient elution, the flow rate was 0. 8 ml·min-1 and the detection wavelength was 334 nm at the column temperature of 25℃and the injection volume was 10 μl. Results:The linear range of viceninⅡ, isoschaftoside and schaftoside was 0.130-3.110 μg(r =0.999 8), 0.180-4.540 μg(r =0.999 9) and 0.080-1.970 μg(r =0. 9999), respectively. The average recovery was 96.8% (RSD =1.9%), 99.2% (RSD =1. 6%) and 97. 1% (RSD=1. 6%) (n=6), respectively. The above three kinds of water-soluble flavonoids from seedling stage to significant accumulation, isoschaftoside and schaftoside reached the highest value in March and August, the content of VitexinⅡ in January and April tends to be stable, in May began to increase gradually, reached the maximum value in August, then began to de-crease. Conclusion:With the contents of water-soluble flavonoids as the indicators, the best harvest time of Isodon lophanthoides is March and August, and the quality of the medicinal materials harvested in August is the best.
