中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
6期
897-900
,共4页
羽扇豆醇%肝细胞癌%Caspase通路
羽扇豆醇%肝細胞癌%Caspase通路
우선두순%간세포암%Caspase통로
Lupeol%Hepatocellular carcinoma%Caspase pathway
目的：：研究羽扇豆醇( Lupeol)在人高转移肝癌细胞系HCCLM3中的抗增殖作用机制。方法：运用CCK-8法检测不同浓度Lupeol在12-48 h对HCCLM3细胞活力的影响及相关Caspase参与Lupeol(20~100μmol·L-1)诱导的细胞凋亡类型；运用Realtime PCR评价Lupeol对细胞内Caspase家族及Bcl-2相关基因的mRNA表达的影响；同时运用流式细胞术检测Lupe-ol对细胞周期分布的影响。结果：Lupeol在24~48 h能够抑制HCCLM3的细胞增殖，并呈现浓度依赖性，在24 h处理细胞的IC50为93μmol·L-1；运用60~100μmol·L-1的Lupeol能够使HCCLM3细胞在G2/M期细胞数增加1倍；Lupeol能够活化Caspase通路，与对照组相比Lupeol处理后细胞内Caspase-3的mRNA表达量增加50%~150%，同时100μmol·L-1的Lupeol处理细胞后使胞内p53及Bax的mRNA表达量分别上调1倍以上，并显著降低Bcl-2及PARP的mRNA水平( P<0.05或P<0.01)。结论：Lupeol具有抑制肝癌细胞增殖能力，对肝癌预防及治疗可能具有一定的协同作用。
目的：：研究羽扇豆醇( Lupeol)在人高轉移肝癌細胞繫HCCLM3中的抗增殖作用機製。方法：運用CCK-8法檢測不同濃度Lupeol在12-48 h對HCCLM3細胞活力的影響及相關Caspase參與Lupeol(20~100μmol·L-1)誘導的細胞凋亡類型；運用Realtime PCR評價Lupeol對細胞內Caspase傢族及Bcl-2相關基因的mRNA錶達的影響；同時運用流式細胞術檢測Lupe-ol對細胞週期分佈的影響。結果：Lupeol在24~48 h能夠抑製HCCLM3的細胞增殖，併呈現濃度依賴性，在24 h處理細胞的IC50為93μmol·L-1；運用60~100μmol·L-1的Lupeol能夠使HCCLM3細胞在G2/M期細胞數增加1倍；Lupeol能夠活化Caspase通路，與對照組相比Lupeol處理後細胞內Caspase-3的mRNA錶達量增加50%~150%，同時100μmol·L-1的Lupeol處理細胞後使胞內p53及Bax的mRNA錶達量分彆上調1倍以上，併顯著降低Bcl-2及PARP的mRNA水平( P<0.05或P<0.01)。結論：Lupeol具有抑製肝癌細胞增殖能力，對肝癌預防及治療可能具有一定的協同作用。
목적：：연구우선두순( Lupeol)재인고전이간암세포계HCCLM3중적항증식작용궤제。방법：운용CCK-8법검측불동농도Lupeol재12-48 h대HCCLM3세포활력적영향급상관Caspase삼여Lupeol(20~100μmol·L-1)유도적세포조망류형；운용Realtime PCR평개Lupeol대세포내Caspase가족급Bcl-2상관기인적mRNA표체적영향；동시운용류식세포술검측Lupe-ol대세포주기분포적영향。결과：Lupeol재24~48 h능구억제HCCLM3적세포증식，병정현농도의뢰성，재24 h처리세포적IC50위93μmol·L-1；운용60~100μmol·L-1적Lupeol능구사HCCLM3세포재G2/M기세포수증가1배；Lupeol능구활화Caspase통로，여대조조상비Lupeol처리후세포내Caspase-3적mRNA표체량증가50%~150%，동시100μmol·L-1적Lupeol처리세포후사포내p53급Bax적mRNA표체량분별상조1배이상，병현저강저Bcl-2급PARP적mRNA수평( P<0.05혹P<0.01)。결론：Lupeol구유억제간암세포증식능력，대간암예방급치료가능구유일정적협동작용。
Objective:To study the mechanism of anti-proliferative effect of lupeol on highly metastatic human hepatocellular car-cinoma HCCLM3 cells. Methods:CCK-8 assay was performed to evaluate the effects of lupeol at different concentration on cell viability in 12-48 h. Caspase inhibitors were used to identify the subtypes of caspases activated during lupeol-induced cell death. The effects of lupeol on the mRNA expression of caspase family and Bcl-2 related genes were detected by real-time PCR. The effects of lupeol on HC-CLM3 cell phase distribution were investigated by flow cytometry. Results:Compared with the control group, lupeol could inhibit HC-CLM3 cell proliferation in a concentration-dependent manner with IC50 of 93 μmol·L-1 in 24h. The number of HCCLM3 cells in the period of G2/M was increased by 1-fold when the lupeol concentration was within 60-100 μmol·L-1 . Lupeol could activate the path-way of caspase, and the mRNA expression of caspase-3 was elevated by 50%-150% when compared with that in the control group. Mo-reover, the mRNA expression of p53 and Bax were increased above 1-fold by lupeol at 100 μmol·L-1 , and the Bcl-2 and PARP ex-pression were significantly suppressed by lupeol at 60-100 μmol·L-1(P<0. 05 or P<0. 01). Conclusion:The results indicate that lupeol has anti-proliferative effect on the liver cancer cells, which is beneficial to the prevention and treatment of liver cancer.
