CAJ | 학술논문

目的：建立江香薷药材中游离型与结合型黄酮的含量测定方法。方法：色谱条件：色谱柱：Dikma Diamonsil C18（2）（250mm ×4．6mm，5μm）；流动相：甲醇－0．1％磷酸（54∶46）；流速：1．0mL／min；检测波长：350nm；柱温：30℃，理论板数按木犀草素峰和山奈素峰计算应不低于3000。结果：木犀草素、山奈素分别在2．436～121．8μg／mL，1．562～78．1μg／mL范围内线性关系良好。木犀草素和山奈素加样回收率分别为100．30％，100．72％；RSD分别为1．61％，1．45％。结论：HPLC方法操作简单，重现性好，可用于江香薷药材的质量控制。
목적：건립강향유약재중유리형여결합형황동적함량측정방법。방법：색보조건：색보주：Dikma Diamonsil C18（2）（250mm ×4．6mm，5μm）；류동상：갑순－0．1％린산（54∶46）；류속：1．0mL／min；검측파장：350nm；주온：30℃，이론판수안목서초소봉화산내소봉계산응불저우3000。결과：목서초소、산내소분별재2．436～121．8μg／mL，1．562～78．1μg／mL범위내선성관계량호。목서초소화산내소가양회수솔분별위100．30％，100．72％；RSD분별위1．61％，1．45％。결론：HPLC방법조작간단，중현성호，가용우강향유약재적질량공제。
Objective:To establish a method of HPLC for determination free and combined flavone in Moslae Chinensis 'jiangxiangru'. Methods:Using a Dikma Diamonsil C18(2)column (4.6mm ×250mm,5μm), the mobile phases were consisted of acetonitrile-0.1%H3PO4(54∶46), with 1.0mL/min flow rate.The detection wavelength was 350nm.The column tempera was 30 centigrade.Results:There were good linearities in the range 2.436~121.8 g/mL of Luteolin (r=0.999 9) and 1.526~78.1 g/mL of Kaempferol (r=0.999 9).The average recovery of Luteolin and Kaempferol were 100.30%and 100.72%in total flavone(n=6);RSD were 1.61%and 1.45%in total flavone.Conclusion:The methods established are feasible and reproducible,and can be used to control the quality of Moslae Chinensis'jiangxiangru'.