CAJ | 학술논문

目的：建立丹桂热淋丸中主要药味的薄层鉴别及含量测定方法，以提高制剂质量标准。方法：采用TLC法对丹桂热淋丸中金银花、桂枝进行了定性鉴别研究；采用HPLC法测定制剂中蛇床子素的含量，选用：Kromasil ODS C18（4.6mm×250mm，5μm）色谱柱，甲醇-水(65∶35)为流动相，流速1.0mL·min-1,检测波长：322nm。结果：TLC法可鉴别出金银花、桂枝的特征斑点，且专属性强，斑点清晰，阴性对照无干扰；蛇床子素在0.0382～0.2292μg范围内与峰面积呈良好的线性关系（r=0.9997），平均回收率为100.09%，RSD=0.92%。结论：该方法准确可靠，可用于丹桂热淋丸的质量控制。
목적：건립단계열림환중주요약미적박층감별급함량측정방법，이제고제제질량표준。방법：채용TLC법대단계열림환중금은화、계지진행료정성감별연구；채용HPLC법측정제제중사상자소적함량，선용：Kromasil ODS C18（4.6mm×250mm，5μm）색보주，갑순-수(65∶35)위류동상，류속1.0mL·min-1,검측파장：322nm。결과：TLC법가감별출금은화、계지적특정반점，차전속성강，반점청석，음성대조무간우；사상자소재0.0382～0.2292μg범위내여봉면적정량호적선성관계（r=0.9997），평균회수솔위100.09%，RSD=0.92%。결론：해방법준학가고，가용우단계열림환적질량공제。
Object:To set up the method of the thin layer chromatography differentiation and content determination of the main in-gredients in a prescription for advancing quality Standard. Methods:TLC was used to quantitative identified for Lonicerae Japonicae Flos、Cinnamomi Ramulus;To set up Content determination method of Osthole by HPLC,Kromasil ODS C18 chromatographic column (4.6mm×250mm,5μm)was used,mobile phase:methanol-water(65:35)as mobile phase,flow rate:1mL·min-1,detection wavelength:322nm. Results:The characteristic spots on TLC plates was Distinguished without interference in the blank reference.The method has highly specific.The linear range of Osthole was 0.0382-0.2292μgμg(r=0.9997).The average recover is 100.09%,RSD=0.92%. Conclu-sion:The method is accurate and reliable.It can use to control the quality of the Dangui Relin water pills.