国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2015年
12期
1644-1647
,共4页
陈杰%彭健%张卓然%程国平
陳傑%彭健%張卓然%程國平
진걸%팽건%장탁연%정국평
支气管哮喘%白介素33%白介素35
支氣管哮喘%白介素33%白介素35
지기관효천%백개소33%백개소35
Bronchial asthma%Interleukin-33%Interleukin-35
目的 研究支气管哮喘患者血清IL-33、IL-35的表达水平,分析IL-33、IL-35间相关性.方法 采用酶联免疫法(ELISA)检测81例非细菌性感染所致中-重度支气管哮喘急性发作期患者治疗前及80名正常对照者血清IL-35、IL-33水平,采用血细胞分析仪检测白细胞数(WBC)、中性粒细胞绝对值(N)、嗜酸性粒细胞绝对值(EOS).细胞培养实验分为空白对照组、细胞刺激素组和IL-35刺激组,ELISA法检测其培养上清IL-33浓度.统计分析支气管哮喘患者急性发作期与正常对照组血清中IL-35、IL-33、WBC、N、EOS,并分析IL-35、IL-33间的相关性.结果 ①哮喘组血WBC、N及EOS计数均高于正常对照组[(7.82±2.93)×109/L vs.(6.38±2.14)×109/L;(5.63±1.47)×109/L vs.(4.57±1.22)×109/L;(0.43±0.27)×109/L vs.(0.22±0.15)×109/L;P＜0.001].②哮喘组血清IL-33水平较正常对照组明显升高[(217.26±52.31)ng/L vs.(105.43±31.64)ng/L],IL-35水平较对照组明显降低[(156.71±24.29)ng/L vs.(311.62±35.28)ng/L],差异有统计学意义(P＜0.001);总体IL-35与IL-33水平呈负相关(r=-0.803,P＜0.05).③哮喘组经过IL-35刺激IL-33水平与仅细胞刺激素相比更低[(15.08±1.92)ng/L vs.(57.33±15.62)ng/L],差异有统计学意义(P＜0.01).结论 IL-35、IL-33均参与了支气管哮喘的发病,支气管哮喘发作期存在各细胞因子间调节失衡.
目的 研究支氣管哮喘患者血清IL-33、IL-35的錶達水平,分析IL-33、IL-35間相關性.方法 採用酶聯免疫法(ELISA)檢測81例非細菌性感染所緻中-重度支氣管哮喘急性髮作期患者治療前及80名正常對照者血清IL-35、IL-33水平,採用血細胞分析儀檢測白細胞數(WBC)、中性粒細胞絕對值(N)、嗜痠性粒細胞絕對值(EOS).細胞培養實驗分為空白對照組、細胞刺激素組和IL-35刺激組,ELISA法檢測其培養上清IL-33濃度.統計分析支氣管哮喘患者急性髮作期與正常對照組血清中IL-35、IL-33、WBC、N、EOS,併分析IL-35、IL-33間的相關性.結果 ①哮喘組血WBC、N及EOS計數均高于正常對照組[(7.82±2.93)×109/L vs.(6.38±2.14)×109/L;(5.63±1.47)×109/L vs.(4.57±1.22)×109/L;(0.43±0.27)×109/L vs.(0.22±0.15)×109/L;P＜0.001].②哮喘組血清IL-33水平較正常對照組明顯升高[(217.26±52.31)ng/L vs.(105.43±31.64)ng/L],IL-35水平較對照組明顯降低[(156.71±24.29)ng/L vs.(311.62±35.28)ng/L],差異有統計學意義(P＜0.001);總體IL-35與IL-33水平呈負相關(r=-0.803,P＜0.05).③哮喘組經過IL-35刺激IL-33水平與僅細胞刺激素相比更低[(15.08±1.92)ng/L vs.(57.33±15.62)ng/L],差異有統計學意義(P＜0.01).結論 IL-35、IL-33均參與瞭支氣管哮喘的髮病,支氣管哮喘髮作期存在各細胞因子間調節失衡.
목적 연구지기관효천환자혈청IL-33、IL-35적표체수평,분석IL-33、IL-35간상관성.방법 채용매련면역법(ELISA)검측81례비세균성감염소치중-중도지기관효천급성발작기환자치료전급80명정상대조자혈청IL-35、IL-33수평,채용혈세포분석의검측백세포수(WBC)、중성립세포절대치(N)、기산성립세포절대치(EOS).세포배양실험분위공백대조조、세포자격소조화IL-35자격조,ELISA법검측기배양상청IL-33농도.통계분석지기관효천환자급성발작기여정상대조조혈청중IL-35、IL-33、WBC、N、EOS,병분석IL-35、IL-33간적상관성.결과 ①효천조혈WBC、N급EOS계수균고우정상대조조[(7.82±2.93)×109/L vs.(6.38±2.14)×109/L;(5.63±1.47)×109/L vs.(4.57±1.22)×109/L;(0.43±0.27)×109/L vs.(0.22±0.15)×109/L;P＜0.001].②효천조혈청IL-33수평교정상대조조명현승고[(217.26±52.31)ng/L vs.(105.43±31.64)ng/L],IL-35수평교대조조명현강저[(156.71±24.29)ng/L vs.(311.62±35.28)ng/L],차이유통계학의의(P＜0.001);총체IL-35여IL-33수평정부상관(r=-0.803,P＜0.05).③효천조경과IL-35자격IL-33수평여부세포자격소상비경저[(15.08±1.92)ng/L vs.(57.33±15.62)ng/L],차이유통계학의의(P＜0.01).결론 IL-35、IL-33균삼여료지기관효천적발병,지기관효천발작기존재각세포인자간조절실형.
Objective To investigate the expression of serum interleukin-33 (IL-33),interleukin-35 (IL-35) in patients with bronchial asthma and their correlation.Methods The expression of serum IL-33,IL-35 of 81 patients with moderate or severe bronchial asthma in acute episode not caused by bacterial infection were detected with ELISA before treatment,along with 80 normal controls.The numbers of white blood cell (WBC),absolute neutrophil count (N),eosinophil absolute value (EOS) were detected by blood cell analyzer.In cell culture experiments,the plasma sample was divided into 3 groups:control group,cell stimulating hormone group,and IL-35 group.After cell culture experiments,the expression of plasma IL-33 in each group was measured by ELISA.Compared the expression of serum IL-35,IL-33,WBC,N,EOS in patients with bronchial asthma in acute episode and normal controls,analyzed the correlation between IL-35 and IL-33.Results ① The numbers of WBC,N,EOS of bronchial asthma group were higher than those of control group [(7.82±2.93)× 109/L vs.(6.38±2.14)× 109/L;(5.63± 1.47)× 109/L vs.(4.57± 1.22)× 109/L;(0.43±0.27)× 109/L vs.(0.22±0.15) × 109/L;P＜0.001].② The expression of serum IL-33 of bronchial asthma group was significantly higher than that of control group [(217.26±52.31)ng/L vs.(105.43±31.64)ng/L],the expression of serum IL-35 was significantly lower than that of control group [(156.71±24.29)ng/L vs.(311.62±35.28)ng/L],with statistically significant differences (P＜0.05).③The expression of serum IL-33 of patients with bronchial asthma undergoing IL-35 stimulating was lower that of patients with bronchial asthma undergoing cell stimulating hormone [(15.08±1.92)ng/L vs.(57.33±15.62)ng/L],with statistically significant difference (P＜0.05).Conclusion IL-35,IL-33 were involved in the pathogenesis of bronchial asthma.Cytokines regulating unbalance existed in acute episode of bronchial asthma.
