中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2015年
16期
13-15
,共3页
张丽娟%李倩%邓海静%张文丽%王小君%裴鑫%郝小惠%李治国%杨方
張麗娟%李倩%鄧海靜%張文麗%王小君%裴鑫%郝小惠%李治國%楊方
장려연%리천%산해정%장문려%왕소군%배흠%학소혜%리치국%양방
血小板源性生长因子%Rho相关卷曲螺旋形成蛋白激酶%α-平滑肌肌动蛋白%肌成纤维细胞%矽肺
血小闆源性生長因子%Rho相關捲麯螺鏇形成蛋白激酶%α-平滑肌肌動蛋白%肌成纖維細胞%矽肺
혈소판원성생장인자%Rho상관권곡라선형성단백격매%α-평활기기동단백%기성섬유세포%석폐
Platelet-derived growth factor%Rho-associated coiled-coil-forming protien kinase%α-smooth muscle action (α-SMA)%Myofibroblast%Silicosis
目的：探讨PDGF/Rock通路介导的肌成纤维细胞分化在大鼠矽肺纤维化形成中的作用。方法：采用非暴露式气管二氧化硅混悬液灌注法制备大鼠矽肺模型，SPF级健康成年Wistar大鼠40只随机分为四组：模型对照4周组、模型对照8周组、矽肺模型4周组、矽肺模型8周组，每组10只。Western blot检测肺组织内α-SMA、phospho-PDGF-β、ROCK、Ⅰ型和Ⅲ型胶原蛋白的表达。结果：大鼠矽肺模型制备成功。与相应对照组相比，矽肺模型组大鼠肺组织内α-SMA、phospho-PDGFR-β、Rock以及Ⅰ型和Ⅲ型胶原蛋白表达均明显增加，比较差异有统计学意义（P<0.05）。结论：PDGF介导的ROCK信号转导通路可能通过促进大鼠肌成纤维细胞转化而促进矽肺大鼠肺组织内的胶原合成，进而促进矽肺纤维化的形成。
目的：探討PDGF/Rock通路介導的肌成纖維細胞分化在大鼠矽肺纖維化形成中的作用。方法：採用非暴露式氣管二氧化硅混懸液灌註法製備大鼠矽肺模型，SPF級健康成年Wistar大鼠40隻隨機分為四組：模型對照4週組、模型對照8週組、矽肺模型4週組、矽肺模型8週組，每組10隻。Western blot檢測肺組織內α-SMA、phospho-PDGF-β、ROCK、Ⅰ型和Ⅲ型膠原蛋白的錶達。結果：大鼠矽肺模型製備成功。與相應對照組相比，矽肺模型組大鼠肺組織內α-SMA、phospho-PDGFR-β、Rock以及Ⅰ型和Ⅲ型膠原蛋白錶達均明顯增加，比較差異有統計學意義（P<0.05）。結論：PDGF介導的ROCK信號轉導通路可能通過促進大鼠肌成纖維細胞轉化而促進矽肺大鼠肺組織內的膠原閤成，進而促進矽肺纖維化的形成。
목적：탐토PDGF/Rock통로개도적기성섬유세포분화재대서석폐섬유화형성중적작용。방법：채용비폭로식기관이양화규혼현액관주법제비대서석폐모형，SPF급건강성년Wistar대서40지수궤분위사조：모형대조4주조、모형대조8주조、석폐모형4주조、석폐모형8주조，매조10지。Western blot검측폐조직내α-SMA、phospho-PDGF-β、ROCK、Ⅰ형화Ⅲ형효원단백적표체。결과：대서석폐모형제비성공。여상응대조조상비，석폐모형조대서폐조직내α-SMA、phospho-PDGFR-β、Rock이급Ⅰ형화Ⅲ형효원단백표체균명현증가，비교차이유통계학의의（P<0.05）。결론：PDGF개도적ROCK신호전도통로가능통과촉진대서기성섬유세포전화이촉진석폐대서폐조직내적효원합성，진이촉진석폐섬유화적형성。
Objective:To investigate the role of myofibroblast’s differentiation mediated by PDGF/Rock pathway in the pathogenesis of pulmonary fibrosis.Method:SiO2 powders were douched in bronchial tube of rat to make the silicotic model. Fourty Wistar rats were randomly divided into 4 groups,including normal control 4 weeks group,normal control 8 weeks group,silicosic model 4 weeks group,silicosic model 8 weeks group.The expression and distribution of phospho-PDGFR-β were observed by immunocytochemistry.Expressions of α-smooth muscle actin (α-SMA),phospho-PDGFR-β,Rock, collagenⅠand collagen Ⅲ were detected by Western blot.Result:Model of rats with silicosis was successful established.Compared with the corresponding control groups,the expressions of α-SMA,phospho-PDGFR-β,ROCK,type Ⅰand type Ⅲ collagen in pulmonary tissues of silicosic model groups increased significantly (P<0.05).Conclusion:PDGF/Rock pathway may play an important role in the development of silicosis by promoting myofibroblast differentiation and further secreting more collgen.
