临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2015年
11期
890-893
,共4页
冠心病%肝细胞生长因子%体外培养%外周血内皮祖细胞
冠心病%肝細胞生長因子%體外培養%外週血內皮祖細胞
관심병%간세포생장인자%체외배양%외주혈내피조세포
Coronary heart disease%Hepatocyte growth factor%Vitro culture%Peripheral blood endothelial progenitor cells
目的：探讨肝细胞生长因子（ HGF）对体外培养冠心病患者外周血内皮祖细胞（ EPCS）生物学功能的影响。方法选取2014年1月至2014年9月就诊的冠心病患者16例作为研究组，同时期未患有冠心病的健康体检者16例作为对照组。每组又均再随机分为HGF组和非HGF组。观察并比较两组患者外周血中CD133+细胞数量、血浆中HGF含量以及EPCS生物学功能。结果研究组患者CD133+细胞数量显著低于对照组患者（ P <0.05）；研究组患者HGF含量显著高于对照组患者（ P <0.05）。研究组非HGF干预增殖能力吸光度、粘附细胞数和迁移细胞数均显著低于对照组非HGF干预组（均P <0.05）。研究组患者中HGF干预组增殖能力吸光度、粘附细胞数和迁移细胞数均显著高于非HGF干预组（均P <0.05）。对照组患者HGF干预组增殖能力吸光度显著高于非HGF干预组（ P <0.05），粘附细胞数和迁移细胞数比较差异均无统计学意义（均P >0.05）。结论 HGF可显著增强冠心病患者外周血中EPCs增殖、粘附和迁移等生物学功能。
目的：探討肝細胞生長因子（ HGF）對體外培養冠心病患者外週血內皮祖細胞（ EPCS）生物學功能的影響。方法選取2014年1月至2014年9月就診的冠心病患者16例作為研究組，同時期未患有冠心病的健康體檢者16例作為對照組。每組又均再隨機分為HGF組和非HGF組。觀察併比較兩組患者外週血中CD133+細胞數量、血漿中HGF含量以及EPCS生物學功能。結果研究組患者CD133+細胞數量顯著低于對照組患者（ P <0.05）；研究組患者HGF含量顯著高于對照組患者（ P <0.05）。研究組非HGF榦預增殖能力吸光度、粘附細胞數和遷移細胞數均顯著低于對照組非HGF榦預組（均P <0.05）。研究組患者中HGF榦預組增殖能力吸光度、粘附細胞數和遷移細胞數均顯著高于非HGF榦預組（均P <0.05）。對照組患者HGF榦預組增殖能力吸光度顯著高于非HGF榦預組（ P <0.05），粘附細胞數和遷移細胞數比較差異均無統計學意義（均P >0.05）。結論 HGF可顯著增彊冠心病患者外週血中EPCs增殖、粘附和遷移等生物學功能。
목적：탐토간세포생장인자（ HGF）대체외배양관심병환자외주혈내피조세포（ EPCS）생물학공능적영향。방법선취2014년1월지2014년9월취진적관심병환자16례작위연구조，동시기미환유관심병적건강체검자16례작위대조조。매조우균재수궤분위HGF조화비HGF조。관찰병비교량조환자외주혈중CD133+세포수량、혈장중HGF함량이급EPCS생물학공능。결과연구조환자CD133+세포수량현저저우대조조환자（ P <0.05）；연구조환자HGF함량현저고우대조조환자（ P <0.05）。연구조비HGF간예증식능력흡광도、점부세포수화천이세포수균현저저우대조조비HGF간예조（균P <0.05）。연구조환자중HGF간예조증식능력흡광도、점부세포수화천이세포수균현저고우비HGF간예조（균P <0.05）。대조조환자HGF간예조증식능력흡광도현저고우비HGF간예조（ P <0.05），점부세포수화천이세포수비교차이균무통계학의의（균P >0.05）。결론 HGF가현저증강관심병환자외주혈중EPCs증식、점부화천이등생물학공능。
Objective To investigate the effect of hepatocyte growth factor( HGF)on biology function of endothelial progenitor cells cul-turing in vitro in peripheral blood of patients with coronary heart disease( EPCS). Methods 16 cases of coronary heart disease patients in Januar-y to September 2014 in our hospital were considered as the research group. 16 cases of healthy people at the same time period without suffering from coronary heart disease were considered as the control group. Each group was then randomly divided into HGF group and non HGF group. The number of CD133+cells in the peripheral blood,content of HGF in the plasma and biological function of EPCS were observed and compared be-tween the two groups of patients. Results The number of CD133+ cells in patients of the study group was significantly lower than that of the con-trol group( P <0. 05). The content of HGF in the study group was significantly higher than those of control group( P <0. 05). Intervention pro-liferation ability of absorbance,cell adhesion and migration of cell number of non HGF group in the study group was significantly lower than the non HGF group of the control group(all P <0. 05). Intervention proliferation ability of absorbance,cell adhesion and migration of cell number in the HGF group was significantly higher than that of non HGF intervention group of the study group( P <0. 05). Intervention proliferation ability of absorbance in the HGF intervention group was significantly higher than that of non HGF intervention group of the control group( P <0. 05),the number of cell adhesion and migration of cell number were not significant( P >0. 05). Conclusion HGF can significantly enhance EPCs prolif-eration,adhesion and migration and other biological functions in peripheral blood of patients with coronary heart disease.
