CAJ | 학술논문

目的：研究骨细胞特异性因子2(Periostin)在肾细胞癌组织中的表达，探讨其与肾细胞癌临床病理因素和预后的关系及意义。方法应用Western blot和Real-time PCR检测90例肾细胞癌组织及相对应的癌旁肾脏组织中Periostin表达情况，并与临床病理资料及预后进行相关性分析。结果76.67%(69/90)的肾癌组织的Periostin表达高于相对应的癌旁组织，65.56%(59/90)肾癌组织的Periostin mRNA表达高于相对应的癌旁组织，肾细胞癌组织Periostin高表达与肿瘤大小、肾盂侵犯、癌栓、淋巴转移相关(P<0.05)，肾细胞癌组织Periostin高表达的患者术后生存时间显著短于Periostin低表达者(P<0.01)。结论 Periostin表达升高与肾细胞癌的增殖、转移、浸润与生存时间密切相关，Periostin可作为预测肾细胞癌转移和判断预后的指标。
목적：연구골세포특이성인자2(Periostin)재신세포암조직중적표체，탐토기여신세포암림상병리인소화예후적관계급의의。방법응용Western blot화Real-time PCR검측90례신세포암조직급상대응적암방신장조직중Periostin표체정황，병여림상병리자료급예후진행상관성분석。결과76.67%(69/90)적신암조직적Periostin표체고우상대응적암방조직，65.56%(59/90)신암조직적Periostin mRNA표체고우상대응적암방조직，신세포암조직Periostin고표체여종류대소、신우침범、암전、림파전이상관(P<0.05)，신세포암조직Periostin고표체적환자술후생존시간현저단우Periostin저표체자(P<0.01)。결론 Periostin표체승고여신세포암적증식、전이、침윤여생존시간밀절상관，Periostin가작위예측신세포암전이화판단예후적지표。
Objective To evaluate the mRNA and preotein levels of Periostin in renal cell carcinoma (RCC), and to analyze the relationships between its protein level and the clinical pathological features, prognosis of RCC. Methods Western blot and real-time PCR were applied to detect the expression levels of Periostin in 90 cases of RCC and the adjacent tissues. The correlations between expression of Periostin and pathological features, prognosis were analyzed. Results 76.67%(69/90) and 65.56%(59/90) patients showed significant higher protein and mRNA lev-els in RCC than adjacent tissues, respectively. The abnormal expression of Periostin correlated with tumor size, renal pel-vis invasion, tumor thrombus and lymph metastasis (P<0.05). Patients with elevated expression of Periostin in RCC had significantly shorter postoperative survival time than those with low expression of Periostin (P<0.01). Conclusion The high expression of Periostin correlates with proliferation, invasion, metastasis and patient prognosis. Our results suggest that Periostin may be a potential marker for determining the degree of malignancy and prognosis.