临床医药实践
臨床醫藥實踐
림상의약실천
PROCEEDING OF CLINICAL MEDICINE
2015年
6期
438-441
,共4页
肺纤维化%丹参%血管内皮生长因子
肺纖維化%丹參%血管內皮生長因子
폐섬유화%단삼%혈관내피생장인자
pulmonary fibrosis%danshen%vascular endothelial growth factor
目的：探讨丹参对肺间质纤维化早中期的干预效果及可能机制。方法：将40只Wistar大鼠随机分为4组：手术对照组、模型组、泼尼松治疗组和丹参治疗组。气管内注射博来霉素建立肺间质纤维化大鼠模型，各组大鼠每日分别灌胃服用生理盐水、泼尼松、中药丹参煎剂，共15 d。术后第16天采取各组大鼠肺组织标本，行苏木精—伊红染色法（HE染色）、Van Gieso（VG）染色观察肺组织病理改变，采用酶联免疫吸附法（ELISA）检测其血管内皮生长因子（ VEGF）水平。结果：模型组大鼠肺组织发生纤维化改变，而泼尼松治疗组和丹参治疗组的肺组织纤维化病变程度较模型组减轻，VEGF水平降低（P＜0.05）。结论：中药丹参水煎剂可减轻肺组织纤维化程度，抑制肺组织VEGF表达上调。
目的：探討丹參對肺間質纖維化早中期的榦預效果及可能機製。方法：將40隻Wistar大鼠隨機分為4組：手術對照組、模型組、潑尼鬆治療組和丹參治療組。氣管內註射博來黴素建立肺間質纖維化大鼠模型，各組大鼠每日分彆灌胃服用生理鹽水、潑尼鬆、中藥丹參煎劑，共15 d。術後第16天採取各組大鼠肺組織標本，行囌木精—伊紅染色法（HE染色）、Van Gieso（VG）染色觀察肺組織病理改變，採用酶聯免疫吸附法（ELISA）檢測其血管內皮生長因子（ VEGF）水平。結果：模型組大鼠肺組織髮生纖維化改變，而潑尼鬆治療組和丹參治療組的肺組織纖維化病變程度較模型組減輕，VEGF水平降低（P＜0.05）。結論：中藥丹參水煎劑可減輕肺組織纖維化程度，抑製肺組織VEGF錶達上調。
목적：탐토단삼대폐간질섬유화조중기적간예효과급가능궤제。방법：장40지Wistar대서수궤분위4조：수술대조조、모형조、발니송치료조화단삼치료조。기관내주사박래매소건립폐간질섬유화대서모형，각조대서매일분별관위복용생리염수、발니송、중약단삼전제，공15 d。술후제16천채취각조대서폐조직표본，행소목정—이홍염색법（HE염색）、Van Gieso（VG）염색관찰폐조직병리개변，채용매련면역흡부법（ELISA）검측기혈관내피생장인자（ VEGF）수평。결과：모형조대서폐조직발생섬유화개변，이발니송치료조화단삼치료조적폐조직섬유화병변정도교모형조감경，VEGF수평강저（P＜0.05）。결론：중약단삼수전제가감경폐조직섬유화정도，억제폐조직VEGF표체상조。
Objective:To explore the effects of Danshen on pulmonary fibrosis in the early and middle stages of the dis-ease and their potential mechanism. Methods:Model rats were induced by injecting bleomycin into tracea. 40 Wistar rats were randomly assigned to 4 groups,which were operation control group,model group,prednisone treatment group and danshen treat-ment group. The rats in operation control group were fed with saline everyday,and the rats in Prednsone treatment and danshen treatment group were fed with prednsone and danshen decoction,respectively. After 16 days,the lung tissues were taken out and observed and vascular endothelial growth factor( VEGF ) were measured by enzyme linked immunosorbent assay( ELISA ) method. Results:Compared with Model group,the two treatment groups had significantly improvement in lung operating find-ings. VEGF were significantly lower in the two treatment groups than that in model group(P<0. 01). Conclusion:Danshen could improve pulmonary fibrosis and the one of potential mechanisms was despressing the expression of VEGF in the lung tis-sue.
