CAJ | 학술논문

目的：通过比较不同途径的酶联免疫斑点（ELISPOT）检测，探讨其对结核性胸膜炎的诊断价值。方法采用T-Spot-TB试剂盒对164例结核性胸腔积液和102例非结核性胸腔积液患者的外周血和胸腔积液标本进行检测。比较两组的斑点形成细胞数（SFC），采用诊断试验比较胸腔积液与外周血ELISPOT检测诊断结核性胸膜炎的灵敏度及特异度。结果胸腔积液与外周血ELISPOT检测的受试者工作特征曲线下面积分别为0.947和0.905；胸腔积液 ELISPOT 检测诊断结核性胸膜炎的灵敏度（95.1%）高于外周血（89.0%）；特异度（90.2%）也高于外周血（88.2%）。结论胸腔积液较外周血ELISPOT检测具有更高的诊断结核性胸膜炎的价值。
목적：통과비교불동도경적매련면역반점（ELISPOT）검측，탐토기대결핵성흉막염적진단개치。방법채용T-Spot-TB시제합대164례결핵성흉강적액화102례비결핵성흉강적액환자적외주혈화흉강적액표본진행검측。비교량조적반점형성세포수（SFC），채용진단시험비교흉강적액여외주혈ELISPOT검측진단결핵성흉막염적령민도급특이도。결과흉강적액여외주혈ELISPOT검측적수시자공작특정곡선하면적분별위0.947화0.905；흉강적액 ELISPOT 검측진단결핵성흉막염적령민도（95.1%）고우외주혈（89.0%）；특이도（90.2%）야고우외주혈（88.2%）。결론흉강적액교외주혈ELISPOT검측구유경고적진단결핵성흉막염적개치。
Objective To evaluate the effects of ELISPOT (enzyme-link immunospot) test using different samples in diagnosing tuberculous pleurisy. Methods Using T-Spot-TB kit to detect interferon-γlevel in pleural effusion and periph?eral blood from 164 patients with tuberculous pleural effusion and 102 patients without tuberculous pleural effusion. Number of spot forming cells (SFCs) as well as the specificity and sensitivity of the tests were compared between these two methods (ELISPOT using leural effusion or peripheral blood). Results The area under the ROC curve was 0.947 in pleural effusion Elispot test while it was 0.905 in peripheral blood Elispot test. The sensitivity of pleural effusion ELISPOT test in diagnosis of tuberculous pleurisy (95.1%) was significantly higher than that of peripheral blood ELISPOT test (89.0%). What’s more, the specificity of pleural effusion ELISPOT test in diagnosis of tuberculous pleurisy (90.2%) was higher than that in diagno?sis of peripheral blood ELISPOT test (88.2%). Conclusion The pleural effusion ELISPOT test is more valuable than periph?eral blood ELISPOT in the diagnosis of tuberculous pleuritis.