CAJ | 학술논문

目的：探讨结核性脑膜炎患者脑脊液及血浆中microRNA (miR)-125b、miR-29a和miR-155-5p的表达水平及其临床意义。方法收集20例结核性脑膜炎患者（结脑组）和20例原发性头痛患者（对照组）的脑脊液和血浆，分别提取总RNA，使用实时定量PCR方法分别检测脑脊液和血浆中miR-125b、miR-29a及miR-155-5p的表达水平。结果与对照组相比，结脑组脑脊液、血浆中miR-29a、miR-125b表达水平明显上调，结脑组血浆中miR-155-5p表达水平明显上调，差异均有统计学意义（均P<0.01），而2组脑脊液中miR-155-5p表达水平比较差异无统计学意义。结论 miR-125b、miR-29a和miR-155-5p可能参与了调节结核性脑膜炎的发生、发展过程，miR-125b和miR-29a有可能作为潜在的诊断结核性脑膜炎的生物学标志。
목적：탐토결핵성뇌막염환자뇌척액급혈장중microRNA (miR)-125b、miR-29a화miR-155-5p적표체수평급기림상의의。방법수집20례결핵성뇌막염환자（결뇌조）화20례원발성두통환자（대조조）적뇌척액화혈장，분별제취총RNA，사용실시정량PCR방법분별검측뇌척액화혈장중miR-125b、miR-29a급miR-155-5p적표체수평。결과여대조조상비，결뇌조뇌척액、혈장중miR-29a、miR-125b표체수평명현상조，결뇌조혈장중miR-155-5p표체수평명현상조，차이균유통계학의의（균P<0.01），이2조뇌척액중miR-155-5p표체수평비교차이무통계학의의。결론 miR-125b、miR-29a화miR-155-5p가능삼여료조절결핵성뇌막염적발생、발전과정，miR-125b화miR-29a유가능작위잠재적진단결핵성뇌막염적생물학표지。
Objective To investigate the expression levels of microRNA(miR)-125b, miR-29a and miR-155-5p in ce?rebrospinal fluid (CSF) and plasma from the patients with tuberculous meningitis and their clinic significance. Methods Cerebrospinal fluid and plasma samples were collected from 20 patients with tuberculous meningitis (tuberculous meningitis group) and 20 patients suffered from primary headache (control group). The total RNAs were extracted.The levels of miR-125b, miR-29a and miR-155-5p were determined by real-time quantitative PCR (q-PCR). Results Levels of miR-29a and miR-125b in both CSF and plasma of patients in tuberculous meningitis group were significantly higher than those in pa?tients from control group with statistical significance (P<0.01). Mean time, the level of miR-155-5p in plasma but not in CSF of patients in tuberculous meningitis group was higher than those in control group with statistical significance ( P<0.01). Conclusion Expression of miR-125b, miR-29a and miR-155-5p may participate in regulating the occurrence and development of tuberculous meningitis. And miR-125b and miR-29a may be used as potential biomarkers for diagnosing tu?berculous meningitis.