天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
6期
635-638,706
,共5页
吴楠%闵鹤鸣%屈惠莹%包翠芬
吳楠%閔鶴鳴%屈惠瑩%包翠芬
오남%민학명%굴혜형%포취분
自噬%磷酸转移酶类(醇族体)%癌,非小细胞肺%自噬相关蛋白1%Ⅲ型磷脂酰肌醇3激酶
自噬%燐痠轉移酶類(醇族體)%癌,非小細胞肺%自噬相關蛋白1%Ⅲ型燐脂酰肌醇3激酶
자서%린산전이매류(순족체)%암,비소세포폐%자서상관단백1%Ⅲ형린지선기순3격매
autophagy%phosphotransferases (alcohol group acceptor)%carcinoma,non-small-cell lung%autophagy relat-ed protein 1%phosphoinositide-3-kinase class 3
目的：探讨自噬基因pULK、PI3KC3在非小细胞肺癌（NSCLC）中的表达及相关性。方法随机抽取NSCLC患者（肺癌组）77例（鳞癌31例、腺癌31例、大细胞未分化癌15例）及其癌旁正常组织21例作为对照。采用免疫组织化学染色及免疫印迹方法定性、定量检测pULK、PI3KC3在NSCLC和癌旁正常肺组织中的表达情况，并采用SPSS 13.0统计软件进行病理因素及相关性分析。结果免疫组化结果显示pULK和PI3KC3阳性表达定位于细胞质。pULK和PI3KC3在肺癌组中的阳性表达(35.1%、40.3%)显著低于癌旁正常肺组织(81.0%、76.2%，P＜0.01)。NSCLC中无淋巴结转移、TNM分期的Ⅰ～Ⅱ期和中高分化患者的pULK和PI3KC3蛋白表达阳性率均较高（P＜0.01），而不同年龄、性别、肿瘤直径大小的NSCLC的表达差异无统计学意义。相关性分析显示pULK与PI3KC3表达呈正相关关系。结论 pULK、PI3KC3在NSCLC中呈低表达。其表达与患者的临床分期、分化程度及淋巴结转移有关，而与患者年龄、性别、肿瘤组织学类型及大小无关。
目的：探討自噬基因pULK、PI3KC3在非小細胞肺癌（NSCLC）中的錶達及相關性。方法隨機抽取NSCLC患者（肺癌組）77例（鱗癌31例、腺癌31例、大細胞未分化癌15例）及其癌徬正常組織21例作為對照。採用免疫組織化學染色及免疫印跡方法定性、定量檢測pULK、PI3KC3在NSCLC和癌徬正常肺組織中的錶達情況，併採用SPSS 13.0統計軟件進行病理因素及相關性分析。結果免疫組化結果顯示pULK和PI3KC3暘性錶達定位于細胞質。pULK和PI3KC3在肺癌組中的暘性錶達(35.1%、40.3%)顯著低于癌徬正常肺組織(81.0%、76.2%，P＜0.01)。NSCLC中無淋巴結轉移、TNM分期的Ⅰ～Ⅱ期和中高分化患者的pULK和PI3KC3蛋白錶達暘性率均較高（P＜0.01），而不同年齡、性彆、腫瘤直徑大小的NSCLC的錶達差異無統計學意義。相關性分析顯示pULK與PI3KC3錶達呈正相關關繫。結論 pULK、PI3KC3在NSCLC中呈低錶達。其錶達與患者的臨床分期、分化程度及淋巴結轉移有關，而與患者年齡、性彆、腫瘤組織學類型及大小無關。
목적：탐토자서기인pULK、PI3KC3재비소세포폐암（NSCLC）중적표체급상관성。방법수궤추취NSCLC환자（폐암조）77례（린암31례、선암31례、대세포미분화암15례）급기암방정상조직21례작위대조。채용면역조직화학염색급면역인적방법정성、정량검측pULK、PI3KC3재NSCLC화암방정상폐조직중적표체정황，병채용SPSS 13.0통계연건진행병리인소급상관성분석。결과면역조화결과현시pULK화PI3KC3양성표체정위우세포질。pULK화PI3KC3재폐암조중적양성표체(35.1%、40.3%)현저저우암방정상폐조직(81.0%、76.2%，P＜0.01)。NSCLC중무림파결전이、TNM분기적Ⅰ～Ⅱ기화중고분화환자적pULK화PI3KC3단백표체양성솔균교고（P＜0.01），이불동년령、성별、종류직경대소적NSCLC적표체차이무통계학의의。상관성분석현시pULK여PI3KC3표체정정상관관계。결론 pULK、PI3KC3재NSCLC중정저표체。기표체여환자적림상분기、분화정도급림파결전이유관，이여환자년령、성별、종류조직학류형급대소무관。
Objective To examine expression levels of autophagy gene pULK and PI3KC3 and to explore their correla?tion with non-small cell lung cancer (NSCLC). Methods A total of 77 samples of surgical resection from NSCLC speci? mens (including 31 cases of squamous cell carcinoma, 31 cases of adenocarcinoma and 15 cases of large cell undifferentiated carcinoma) and 21 samples of same normal lung tissue were randomly selected. Expressions of pULK and PI3KC3 in lung tissues were assessed by immunohistochemistry and Western blot. All dates were analyzed using SPSS13.0 statistical pack?age. Results Immunohistochemistry indicated that pULK and PI3KC3 localized into the cytoplasm. The expression levels of pULK and PI3KC3 are significantly lower in patient with NSCLC than those in peri-tumor tissue (35.1%vs 81.0%, 40.3%vs 76.2%respectively, P<0.01) . Immunohistochemistry and Western bolt analysis confirmed that pULK and PI3KC3 ex?pressions were significantly down-regulated (P<0.01) in patients with low grade cellular differentiation, metastasis of lymph node, or stageⅢandⅣ. And expression levels of pULK and PI3KC3 in NSCLC did not differ significantly with ages, gen?der, tumor size and pathological type. Correlation analysis showed that the expression of PI3KC3 was positively correlated with pULK. Conclusion pULK and PI3KC3 expressions were lower in NSCLC than those in normal lung tissue group. The expression levels of pULK and PI3KC3 in NSCLC were correlated with patient's clinical stage, differentiation grade, lymph node metastasis but were unrelated with age, gender, histological type and size.
