天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
6期
607-610,611
,共5页
蔡英%黄慧玲%范维佳%武俏丽%李晓茜%苏彦华%温晓昶
蔡英%黃慧玲%範維佳%武俏麗%李曉茜%囌彥華%溫曉昶
채영%황혜령%범유가%무초려%리효천%소언화%온효창
牛磺酸%脑损伤%膜转运蛋白质类%脑水肿%水通道蛋白质4%大鼠,Sprague-Dawley%牛磺酸转运体
牛磺痠%腦損傷%膜轉運蛋白質類%腦水腫%水通道蛋白質4%大鼠,Sprague-Dawley%牛磺痠轉運體
우광산%뇌손상%막전운단백질류%뇌수종%수통도단백질4%대서,Sprague-Dawley%우광산전운체
Taurine%brain injuries%membrane transport proteins%brain edema%aquaporin 4%rats,Sprague-Dawley%taurine transporter
目的：研究牛磺酸转运体(TauT)在牛磺酸调节重型颅脑创伤大鼠后期脑水肿中的作用。方法将40只大鼠按随机数字表法分为4组：假手术组（Sham组）、脑外伤组（TBI组）、牛磺酸预防组（Pre-Tau组)和牛磺酸治疗组（Tau组）。液压冲击法制作重型颅脑创伤大鼠模型。Pre-Tau组和Tau组分别在造模前或后给予120 g/L的牛磺酸溶液，其他两组给予等量的等渗生理盐水。7 d后用干湿重法检测脑组织中脑水含量，实时荧光定量PCR和West?ern Blot方法检测TauT和水通道蛋白(AQP4)的mRNA表达及蛋白含量。结果与Sham组相比，TBI组脑水含量、AQP4的mRNA和蛋白表达水平均显著升高，TauT的mRNA和蛋白表达水平显著降低。与TBI组比较，Pre-Tau组和Tau组脑水含量、AQP4的mRNA和蛋白表达水平显著降低、TauT的mRNA表达明显升高；Tau组TauT的蛋白表达明显升高；Pre-Tau组TauT的蛋白表达有所升高，但差异无统计学意义。AQP4的mRNA和蛋白表达水平与脑水含量呈正相关（r分别为0.621和0.457），AQP4的mRNA与蛋白表达亦呈正相关（r=0.459）。结论牛磺酸通过提高重型颅脑创伤大鼠后期TauT的表达水平，降低脑水含量和AQP4的表达，发挥神经元保护作用。
目的：研究牛磺痠轉運體(TauT)在牛磺痠調節重型顱腦創傷大鼠後期腦水腫中的作用。方法將40隻大鼠按隨機數字錶法分為4組：假手術組（Sham組）、腦外傷組（TBI組）、牛磺痠預防組（Pre-Tau組)和牛磺痠治療組（Tau組）。液壓遲擊法製作重型顱腦創傷大鼠模型。Pre-Tau組和Tau組分彆在造模前或後給予120 g/L的牛磺痠溶液，其他兩組給予等量的等滲生理鹽水。7 d後用榦濕重法檢測腦組織中腦水含量，實時熒光定量PCR和West?ern Blot方法檢測TauT和水通道蛋白(AQP4)的mRNA錶達及蛋白含量。結果與Sham組相比，TBI組腦水含量、AQP4的mRNA和蛋白錶達水平均顯著升高，TauT的mRNA和蛋白錶達水平顯著降低。與TBI組比較，Pre-Tau組和Tau組腦水含量、AQP4的mRNA和蛋白錶達水平顯著降低、TauT的mRNA錶達明顯升高；Tau組TauT的蛋白錶達明顯升高；Pre-Tau組TauT的蛋白錶達有所升高，但差異無統計學意義。AQP4的mRNA和蛋白錶達水平與腦水含量呈正相關（r分彆為0.621和0.457），AQP4的mRNA與蛋白錶達亦呈正相關（r=0.459）。結論牛磺痠通過提高重型顱腦創傷大鼠後期TauT的錶達水平，降低腦水含量和AQP4的錶達，髮揮神經元保護作用。
목적：연구우광산전운체(TauT)재우광산조절중형로뇌창상대서후기뇌수종중적작용。방법장40지대서안수궤수자표법분위4조：가수술조（Sham조）、뇌외상조（TBI조）、우광산예방조（Pre-Tau조)화우광산치료조（Tau조）。액압충격법제작중형로뇌창상대서모형。Pre-Tau조화Tau조분별재조모전혹후급여120 g/L적우광산용액，기타량조급여등량적등삼생리염수。7 d후용간습중법검측뇌조직중뇌수함량，실시형광정량PCR화West?ern Blot방법검측TauT화수통도단백(AQP4)적mRNA표체급단백함량。결과여Sham조상비，TBI조뇌수함량、AQP4적mRNA화단백표체수평균현저승고，TauT적mRNA화단백표체수평현저강저。여TBI조비교，Pre-Tau조화Tau조뇌수함량、AQP4적mRNA화단백표체수평현저강저、TauT적mRNA표체명현승고；Tau조TauT적단백표체명현승고；Pre-Tau조TauT적단백표체유소승고，단차이무통계학의의。AQP4적mRNA화단백표체수평여뇌수함량정정상관（r분별위0.621화0.457），AQP4적mRNA여단백표체역정정상관（r=0.459）。결론우광산통과제고중형로뇌창상대서후기TauT적표체수평，강저뇌수함량화AQP4적표체，발휘신경원보호작용。
Objective To investigate the effect of taurine transporter in the process of protection of brain edema in rats with severe traumatic head injury. Methods A total of 24 Male Sprague-Dawley rats were randomly divided into 4 groups. Except the control rats (Group Sham), all other three groups were subjected to lateral fluid percussion head injury. The TBI (Traumatic brain injury) models (Group TBI) and surgical control rats (Group Sham) were injected with saline through caudal vein after surgery, while the Taurine prevention and Taurine treatment models (Group Pre Tau and Group Tau) were injected with 120 g/L taurine solution before or after surgeries respectively. Water content in each brain, mRNA and protein expres?sion of aquaporin 4 and taurine transporter in the injured rat brain hemispheres were all evaluated over the time course of the study (7 d) in each group. Results Compared with rats in Group Sham, water content in each brain increase, mRNA tran?scription and protein expression of AQP4 were both up regulated but the mRNA transcription and protein expression of TauT were both down-regulated in rats in TBI group. Compared with rats in TBI group, brain water content, mRNA transcription and protein expression of AQP4 all decrease while mRNA transcription and protein expression of TauT all increase in rats in Pre tau and Tau groups. There is no statistical difference of TauT expression between rats in pre-tau group and Tau group. Conclusion Taurine exert its neuron protection role through draining water content from brain and down regulating expres?sion of AQP4 but rising expression of TauT after TBI.
