天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
6期
587-589,590
,共4页
王刚涛%张旭辉%张卫东%夏磊
王剛濤%張旭輝%張衛東%夏磊
왕강도%장욱휘%장위동%하뢰
蜕皮甾酮%软骨细胞%脂多糖类%细胞增殖%细胞凋亡%白细胞介素1β%诱导型一氧化氮合酶
蛻皮甾酮%軟骨細胞%脂多糖類%細胞增殖%細胞凋亡%白細胞介素1β%誘導型一氧化氮閤酶
세피치동%연골세포%지다당류%세포증식%세포조망%백세포개소1β%유도형일양화담합매
Ecdysterone%chondrocytes%lipopolysaccharides%cell proliferation%apoptosis%interleukin-1beta%iNOS
目的：探讨蜕皮甾酮（EDS）对脂多糖（LPS）诱导的兔软骨细胞损伤的保护作用及其机制。方法体外分离、培养兔关节软骨细胞，随机分为对照组、LPS诱导损伤组（LPS组），蜕皮甾酮干预组(LPS+EDS组)。MTT法和流式细胞术分别检测各组细胞增殖率及细胞凋亡率；RT-PCR和Western blot检测软骨细胞中诱导型一氧化氮合酶（iNOS）表达；硝酸还原酶法和ELISA法分别检测各组NO及白细胞介素（IL）-1β含量。结果与对照组相比，LPS组细胞增殖率降低，凋亡率升高，iNOS mRNA和蛋白表达量以及NO和IL-1β含量增高(均P<0.05)。LPS+EDS组较LPS组细胞增殖率升高，凋亡率降低，iNOS mRNA和蛋白表达量及NO和IL-1β的含量降低（均P<0.05）。结论蜕皮甾酮对LPS诱导的兔软骨细胞损伤具有保护作用，其保护作用可能与抑制iNOS表达有关。
目的：探討蛻皮甾酮（EDS）對脂多糖（LPS）誘導的兔軟骨細胞損傷的保護作用及其機製。方法體外分離、培養兔關節軟骨細胞，隨機分為對照組、LPS誘導損傷組（LPS組），蛻皮甾酮榦預組(LPS+EDS組)。MTT法和流式細胞術分彆檢測各組細胞增殖率及細胞凋亡率；RT-PCR和Western blot檢測軟骨細胞中誘導型一氧化氮閤酶（iNOS）錶達；硝痠還原酶法和ELISA法分彆檢測各組NO及白細胞介素（IL）-1β含量。結果與對照組相比，LPS組細胞增殖率降低，凋亡率升高，iNOS mRNA和蛋白錶達量以及NO和IL-1β含量增高(均P<0.05)。LPS+EDS組較LPS組細胞增殖率升高，凋亡率降低，iNOS mRNA和蛋白錶達量及NO和IL-1β的含量降低（均P<0.05）。結論蛻皮甾酮對LPS誘導的兔軟骨細胞損傷具有保護作用，其保護作用可能與抑製iNOS錶達有關。
목적：탐토세피치동（EDS）대지다당（LPS）유도적토연골세포손상적보호작용급기궤제。방법체외분리、배양토관절연골세포，수궤분위대조조、LPS유도손상조（LPS조），세피치동간예조(LPS+EDS조)。MTT법화류식세포술분별검측각조세포증식솔급세포조망솔；RT-PCR화Western blot검측연골세포중유도형일양화담합매（iNOS）표체；초산환원매법화ELISA법분별검측각조NO급백세포개소（IL）-1β함량。결과여대조조상비，LPS조세포증식솔강저，조망솔승고，iNOS mRNA화단백표체량이급NO화IL-1β함량증고(균P<0.05)。LPS+EDS조교LPS조세포증식솔승고，조망솔강저，iNOS mRNA화단백표체량급NO화IL-1β적함량강저（균P<0.05）。결론세피치동대LPS유도적토연골세포손상구유보호작용，기보호작용가능여억제iNOS표체유관。
Objective To study the effect of ecdysterone (EDS) on rabbits chondrocytes that is injuried by lipopolysac?charide (LPS). Methods Aricular chondrocytes were isolated from rabbits and randomly divided into three groups:control group;chondrocytes with LPS induced injury (LPS group);injury chondrocytes treated with EDS (LPS+EDS group). The cell proliferation and cell apoptosis of chondrocytes were determined by MTT method and flow cytometry assay respectively. The mRNA and protein expression levels of inducible nitric oxide synthase (iNOS) were detected by RT-PCR and western blot. In addition, the content of NO and IL-1βwere measured by nitric acid reductase assay and enzyme-linked immunosorbent assay (ELISA) respectively. Results Attenuated proliferation, increased cell apoptosis, iNOS, NO and IL-1βwere seen in LPS group , but all these changes were significantly reversed by EDS treatment (P<0.05). Conclusion Ecdysterone exhib?ited a protective effect on LPS induced rabbits chondrocytes injury through inhibiting the expression of iNOS.
