CAJ | 학술논문

【目的】探讨小檗碱对鼻咽癌细胞凋亡和放疗敏感性的影响。【方法】收集鼻咽癌细胞株CEN‐2培养，根据处理方法不同分为6组。A组：空白对照，不做处理；B组：仅单纯2 Gy剂量射线照射，培养24 h；C组：20μg／mL （C1）、40μg／mL （C2）小檗碱药物干预，作用48 h；D组：药物＋射线照射干预，根据药物浓度不同分为20μg／mL小檗碱组（D1），40μg／mL小檗碱组（D2），用法同前。采用流式细胞仪检测各组细胞周期分布及细胞凋亡指数并比较。【结果】D1组、D2组G1期细胞比例少于B组，其差异有显著性（ P＜0．05）；D组G2、S期细胞比例增加，药物浓度增加后上述变化更明显，与B组、C组比较差异均有显著性（ P ＜0．05）；浓度高小檗碱作用细胞凋亡指数高（P＜0．05），且D组凋亡指数显著高于C组（P＜0．05），但B组与C1组比较无显著性差异（P＞0．05）。【结论】小檗碱联合射线照射干预，可促进鼻咽癌细胞CNE‐2的凋亡，增强放射敏感性。
【목적】탐토소벽감대비인암세포조망화방료민감성적영향。【방법】수집비인암세포주CEN‐2배양，근거처리방법불동분위6조。A조：공백대조，불주처리；B조：부단순2 Gy제량사선조사，배양24 h；C조：20μg／mL （C1）、40μg／mL （C2）소벽감약물간예，작용48 h；D조：약물＋사선조사간예，근거약물농도불동분위20μg／mL소벽감조（D1），40μg／mL소벽감조（D2），용법동전。채용류식세포의검측각조세포주기분포급세포조망지수병비교。【결과】D1조、D2조G1기세포비례소우B조，기차이유현저성（ P＜0．05）；D조G2、S기세포비례증가，약물농도증가후상술변화경명현，여B조、C조비교차이균유현저성（ P ＜0．05）；농도고소벽감작용세포조망지수고（P＜0．05），차D조조망지수현저고우C조（P＜0．05），단B조여C1조비교무현저성차이（P＞0．05）。【결론】소벽감연합사선조사간예，가촉진비인암세포CNE‐2적조망，증강방사민감성。
[Objective] To explore whether berberine can improve the radiosensitivity of nasopharyngeal carcinoma cells .[Methods]Nasopharyngeal carcinoma CNE‐2 cell lines were cultured in vitro and divided ran‐domly into berberine intervention ,berberine plus irradiation ,irradiation and non‐intervention groups .Berber‐ine and berberine plus irradiation groups were both divided into two subgroups according to different berberine concentrations .Cell cycle distribution and apoptosis were detected by flow cytometry .And the protein expres‐sions of survivin and caspase‐3 were examined by immunocytochemical method (SABC) .[Results] The apop‐totic ratio was 20 .37% in non‐intervention group ,25 .81% in irradiation group ,27 .19% in berberine (20 μg/mL) group ,44 .02% in berberine (20 μg/mL) plus irradiation group and 49 .86% in berberine (40 μg/mL) plus irradiation group .Berberine showed dose‐dependent cytotoxicity .In 20μg berberine + irradiation and 40μ g berberine + irradiation groups ,G1 cell ratio was less than irradiation group .There were significant differ‐ences ( P <0 .05) .In berberine + irradiation group ,the percentage of cells increased in G2 and S phases and the increase of drug concentration was more obvious after the change .Significant differences existed between irradiation and medication groups ( P <0 .05) .[Conclusion] Berberine plus irradiation can accelerate the de‐generation of CNE‐2 cells and indicate its potential role of radiosensitization .