CAJ | 학술논문

【目的】在体外模拟临床肺移植，建立起肺移植缺血再灌注损伤（ischemia reperfusion injury ，IRI）的细胞模型。【方法】将人肺泡上皮细胞系A549用Perfadex液保存于100％氧气，4℃环境中来模拟缺血过程，缺血时间分为6h、12h、18h、24h，然后加入37℃含10％胎牛血清的DM EM培养基来模拟再灌注过程，再灌注时间为2 h。分别检测超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）含量，并应用CCK‐8测细胞存活率。【结果】与对照组相比，各时间点试验组M DA、LD H含量逐渐增加，而SOD含量和细胞存活率则逐渐降低。各时间点MDA、LDH、SOD、细胞存活率水平也存在差异。【结论】成功建立了肺移植IRI细胞模型，此模型能够在分子与细胞水平研究肺移植IRI机制。
【목적】재체외모의림상폐이식，건립기폐이식결혈재관주손상（ischemia reperfusion injury ，IRI）적세포모형。【방법】장인폐포상피세포계A549용Perfadex액보존우100％양기，4℃배경중래모의결혈과정，결혈시간분위6h、12h、18h、24h，연후가입37℃함10％태우혈청적DM EM배양기래모의재관주과정，재관주시간위2 h。분별검측초양화물기화매（SOD）、병이철（MDA）、유산탈경매（LDH）함량，병응용CCK‐8측세포존활솔。【결과】여대조조상비，각시간점시험조M DA、LD H함량축점증가，이SOD함량화세포존활솔칙축점강저。각시간점MDA、LDH、SOD、세포존활솔수평야존재차이。【결론】성공건립료폐이식IRI세포모형，차모형능구재분자여세포수평연구폐이식IRI궤제。
[Objective]To establish a novel cell model imitating clinical lung transplantation that can be applied in the study of Ischemia reperfusion injury (IRI) in lung transplantation .[Methods] A549 cells ,a human pulmonary epithelial cell line ,were preserved in 100% O2 at 4℃ for 6 ,12 ,18 ,and 24h in Perfadex solution ,simulating ische‐mia .Then introduce 37℃ DMEM plus 10% fetal bovine serum to simulate reperfusion for a period of 2 h .The con‐tent of malondialdehyde(MDA) ,superoxide dismutase(SOD) and lactate dehydrogenase(LDH) were measured ,The cell survival rate of A549 was determined by CCK‐8 assay .[Results]The content of MDA、LDH of each experimental group gradually decreased and the content of SOD and the cell survival rate gradually increased than control group . There was difference in the content of MDA ,LDH ,SOD and the cell survival rate in each experimental group .[Con‐clusions] The lung transplantation IRI cell model was successfully established .This model could be used to study mechanisms related to IRI at the cellular and molecular level .