CAJ | 학술논문

艾美耳球虫是一类重要的肠道病原，其裂殖生殖阶段的虫体逸出过程是造成畜禽肠道破坏的主要原因之一，但此逸出过程的机制仍鲜有报道。本研究以乙醇作为诱导剂研究柔嫩艾美耳球虫M2 e株子孢子从宿主细胞中逸出的机制。结果显示，乙醇可诱导子孢子从MDBK细胞中逸出，此逸出过程依赖于虫体的运动能力；同时，乙醇可激发子孢子逸出相关的微线体蛋白2（ Mic2）的分泌释放。进一步实验证实，螯合虫体内部钙离子明显阻断了子孢子逸出及Mic2蛋白的释放。本研究初步证实了与柔嫩艾美耳球虫逸出相关的蛋白和离子，为深入解析球虫致病的分子机制、研发新型抗球虫药物提供了新的研究方向。
애미이구충시일류중요적장도병원，기렬식생식계단적충체일출과정시조성축금장도파배적주요원인지일，단차일출과정적궤제잉선유보도。본연구이을순작위유도제연구유눈애미이구충M2 e주자포자종숙주세포중일출적궤제。결과현시，을순가유도자포자종MDBK세포중일출，차일출과정의뢰우충체적운동능력；동시，을순가격발자포자일출상관적미선체단백2（ Mic2）적분비석방。진일보실험증실，오합충체내부개리자명현조단료자포자일출급Mic2단백적석방。본연구초보증실료여유눈애미이구충일출상관적단백화리자，위심입해석구충치병적분자궤제、연발신형항구충약물제공료신적연구방향。
Apicomplexan parasites infect nearly all vertebrate hosts even including humans and cause some severe diseases such as malaria, toxoplasmosis, cryptosporidiosis which are responsible for substantial economic losses. Like most intracellular pathogens, egress from host cells is a vital step of the apicomplexan parasites, which attracted attentions of many research groups.Unfortunately, as an important genus of Phylum Apicomplexa, little information of egress is known on Eimeria species which leads large amount of losses to poultry industry annually.In this report we used ethanol to induce egress of E.tenella M2e transgenic strain (EtM2e) sporozoites which express yellow fluorescent protein (YFP).Results showed that ethanol could induce egress of EtM2e sporozoites from infected Madin Derby Bovine Kidney ( MDBK) cells, and this process was depended on the mobility of the parasites.We also found that ethanol could also stimulate microneme protein discharge.Furthermore, both the course of egress and the secretion of microneme were controlled by the flux of intracellular Ca2+of the parasite.Taken together, our results preliminarily explained the mechanism of egress of eimerian parasites, which provided clues to the further study.