寄生虫与医学昆虫学报
寄生蟲與醫學昆蟲學報
기생충여의학곤충학보
ACTA PARASITOLOGICA ET MEDICA ENTOMOLOGICA SINICA
2014年
4期
258-262
,共5页
李超%汪运舟%陶鸽如%王丹%焦雅清%顾小龙%索勋%刘贤勇
李超%汪運舟%陶鴿如%王丹%焦雅清%顧小龍%索勛%劉賢勇
리초%왕운주%도합여%왕단%초아청%고소룡%색훈%류현용
肠艾美耳球虫%致病性%免疫原性
腸艾美耳毬蟲%緻病性%免疫原性
장애미이구충%치병성%면역원성
Eimeria intestinalis%Pathogenicity%Immunogenicity
肠艾美耳球虫是兔球虫中的主要致病虫种之一,充分研究其生物学特性对兔球虫病的防治有十分重要的意义。本文对肠艾美耳球虫张家口分离株的致病性和免疫原性进行了初步研究。利用3个剂量(1×104、1×105、1×106)的肠艾美耳球虫张家口分离株孢子化卵囊感染35~45日龄无球虫兔,观察临床症状和体重变化,以研究其致病性;21 d后,再用1×106个肠艾美耳球虫孢子化卵囊进行攻毒,监测体重变化和卵囊排出量,评价其免疫原性。结果发现,接种1×104个卵囊,即可导致家兔出现临床症状,与空白对照组相比,日增重显著下降,更高感染剂量则会加重症状。攻毒后,免疫组增重未受影响,与空白对照组相比无差异,而不免疫攻毒组体重显著下降;同时免疫组的卵囊排出量显著低于不免疫攻毒组。结果表明肠艾美耳球虫张家口分离株对仔兔具有中等致病性;同时其免疫原性良好。因此可以把该株球虫作为原始株进行早熟虫株选育,并以此为基础,进行兔球虫病疫苗的开发。
腸艾美耳毬蟲是兔毬蟲中的主要緻病蟲種之一,充分研究其生物學特性對兔毬蟲病的防治有十分重要的意義。本文對腸艾美耳毬蟲張傢口分離株的緻病性和免疫原性進行瞭初步研究。利用3箇劑量(1×104、1×105、1×106)的腸艾美耳毬蟲張傢口分離株孢子化卵囊感染35~45日齡無毬蟲兔,觀察臨床癥狀和體重變化,以研究其緻病性;21 d後,再用1×106箇腸艾美耳毬蟲孢子化卵囊進行攻毒,鑑測體重變化和卵囊排齣量,評價其免疫原性。結果髮現,接種1×104箇卵囊,即可導緻傢兔齣現臨床癥狀,與空白對照組相比,日增重顯著下降,更高感染劑量則會加重癥狀。攻毒後,免疫組增重未受影響,與空白對照組相比無差異,而不免疫攻毒組體重顯著下降;同時免疫組的卵囊排齣量顯著低于不免疫攻毒組。結果錶明腸艾美耳毬蟲張傢口分離株對仔兔具有中等緻病性;同時其免疫原性良好。因此可以把該株毬蟲作為原始株進行早熟蟲株選育,併以此為基礎,進行兔毬蟲病疫苗的開髮。
장애미이구충시토구충중적주요치병충충지일,충분연구기생물학특성대토구충병적방치유십분중요적의의。본문대장애미이구충장가구분리주적치병성화면역원성진행료초보연구。이용3개제량(1×104、1×105、1×106)적장애미이구충장가구분리주포자화란낭감염35~45일령무구충토,관찰림상증상화체중변화,이연구기치병성;21 d후,재용1×106개장애미이구충포자화란낭진행공독,감측체중변화화란낭배출량,평개기면역원성。결과발현,접충1×104개란낭,즉가도치가토출현림상증상,여공백대조조상비,일증중현저하강,경고감염제량칙회가중증상。공독후,면역조증중미수영향,여공백대조조상비무차이,이불면역공독조체중현저하강;동시면역조적란낭배출량현저저우불면역공독조。결과표명장애미이구충장가구분리주대자토구유중등치병성;동시기면역원성량호。인차가이파해주구충작위원시주진행조숙충주선육,병이차위기출,진행토구충병역묘적개발。
Eimeria intestinalis is one relatively high pathogenic specie of rabbit coccidia.Fully understanding of its biological characteristics makes great contribution to the control of rabbit coccidiosis.In this study, the pathogenicity and immunogenicity of E.intestinalis ZJK strain was studied on weaning rabbits by investigating clinical syndrome, body weight loss and oocyst output.Four groups of rabbits were inoculated with sporulated oocysts in the dose of 0, 1 ×104 , 1 ×105 and 1 ×106, respectively.Then these rabbits were challenged with 1 ×106 oocysts 21 days post inoculation.The results show that the infection with 1 ×104 oocysts was sufficient to cause clinical syndrome and body weight loss, compared with the control group.And the groups given more oocysts show worse symptom.After challenge, no difference of the body weight gain was seen between non-infected control group and immunized groups; meanwhile, the oocyst output of the immunized rabbits was much lower than that of the non-immunized but challenged control group.These results suggest that E. intestinalis ZJK strain is of mediate pathogenicity and excellent immunogenicity. Therefore, a precocious line of E. intestinalis based on this strain can be a suitable candidate for the development of a live attenuated vaccine for rabbit coccidiosis.