CAJ | 학술논문

和缓艾美耳球虫 Eimeria mitis是7种鸡球虫中致病力弱的虫种，对于其免疫原性的认识至今仍有争论。本研究以和缓艾美耳球虫涿州株为研究对象，以初次免疫及二次免疫后鸡的卵囊排出量为标准评估了其免疫原性。同时利用ELISA检测了免疫后鸡群血清中球虫特异性IgY抗体的水平，并通过ELISPOT技术分析了二免后鸡群外周血单核淋巴细胞（ PBMC）中分泌IFN-γ的特异性淋巴细胞的数量。结果显示，二次免疫后，鸡的卵囊排出量显著低于初次免疫，免疫组的血清特异性IgY抗体水平较低，而分泌IFN-γ的特异性淋巴细胞的数量较高。这些结果表明和缓艾美耳球虫涿州株具备良好免疫原性，且其激发宿主产生的保护性免疫以细胞免疫应答为主，有作为疫苗株的潜力。
화완애미이구충 Eimeria mitis시7충계구충중치병력약적충충，대우기면역원성적인식지금잉유쟁론。본연구이화완애미이구충탁주주위연구대상，이초차면역급이차면역후계적란낭배출량위표준평고료기면역원성。동시이용ELISA검측료면역후계군혈청중구충특이성IgY항체적수평，병통과ELISPOT기술분석료이면후계군외주혈단핵림파세포（ PBMC）중분비IFN-γ적특이성림파세포적수량。결과현시，이차면역후，계적란낭배출량현저저우초차면역，면역조적혈청특이성IgY항체수평교저，이분비IFN-γ적특이성림파세포적수량교고。저사결과표명화완애미이구충탁주주구비량호면역원성，차기격발숙주산생적보호성면역이세포면역응답위주，유작위역묘주적잠력。
Eimeria mitis with low pathogenicity is easily ignored compared with other Eimeria spp.in chicken and the mechanism of difference immunogenicity between different strains is unclear.In this study, E.mitis ( Zhuozhou Strain) was used to analysis its immunogenicity by the oocysts output post each immunization.Moreover, We analyzed the humoral immune response and cellular immune response elicited by E.mitis infection via the serum IgY antibody titer detected by ELISA and IFN-γsecretion cell population post stimulated with E.mitis in peripheral blood mononuclear cell ( PBMC) by ELISPOT, respectively.Our results show that the oocyst output was relatively low and the IgY titer was still very low after the second immunization.However, high concentration of IFN-γwas detected in PBMC after stimulated with E.mitis post the second immunization 2 weeks.Our results indicated that strong cellular immune responses elicited by E.mitis could be contributed to its good immunogenicity and thus protect chicken from homologous infection.