CAJ | 학술논문

目的：研究阿尔茨海默病（AD）患者血清脑源性神经营养因子（BDNF）水平的改变及意义。方法对40例AD患者（ AD组）用酶联免疫吸附法检测血清BDNF水平、聚合酶链反应-限制性片段长度多态性对APOE基因进行分型及神经心理评估，并与遗忘型轻度认知障碍（ aMCI组）患者和正常对照者（正常对照组）进行比较，分析AD患者血清BDNF水平及与临床指标的相关性。结果与正常对照组相比， AD和aMCI血清BDNF水平差异无统计学意义（ F＝1．21，P＝0．33）；aMCI、轻度AD、中重度AD组间BDNF水平差异无统计学意义（ F＝2．31，P＝0．08）；相关分析发现是否携带APOEε4等位基因和简易精神状态检查（ MMSE）评分不能影响血清BDNF水平（ P＞0．05）。结论血清BDNF水平既不能作为AD早期诊断的外周标志物，也不能反映AD病情的严重程度。
목적：연구아이자해묵병（AD）환자혈청뇌원성신경영양인자（BDNF）수평적개변급의의。방법대40례AD환자（ AD조）용매련면역흡부법검측혈청BDNF수평、취합매련반응-한제성편단장도다태성대APOE기인진행분형급신경심리평고，병여유망형경도인지장애（ aMCI조）환자화정상대조자（정상대조조）진행비교，분석AD환자혈청BDNF수평급여림상지표적상관성。결과여정상대조조상비， AD화aMCI혈청BDNF수평차이무통계학의의（ F＝1．21，P＝0．33）；aMCI、경도AD、중중도AD조간BDNF수평차이무통계학의의（ F＝2．31，P＝0．08）；상관분석발현시부휴대APOEε4등위기인화간역정신상태검사（ MMSE）평분불능영향혈청BDNF수평（ P＞0．05）。결론혈청BDNF수평기불능작위AD조기진단적외주표지물，야불능반영AD병정적엄중정도。
ObjectiveToinvestigatethechangesofbrain-derivedneurotrophicfactor(BDNF)from peripheral serum in patients with Alzheimer disease ( AD) and evaluate the significance of changes .Methods Forty AD patients ( including 26 mild AD and 14 moderate-severe AD,AD group) were recruited to measure the serum levels of BDNF using enzyme-linked immunosorbent assay ( ELISA) .APOE genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP ) .Cognitive functions were assessed by neuropsychological tests .The results were compared with those of amnestic mild cognitive impairment patients ( aMCI group) and normal controls (NC group).The alteration of serum levels of BDNF and the relationship between BDNF and clinical variables were analyzed in AD patients .Rseults Compared with NC group ,no significant differences for the serum levels of BDNF were observed in AD and aMCI groups ( F=1.21,P=0.33) .the serum levels of BDNF also showed no significant difference among aMCI ,mild AD,moderate-severe AD groups (F=2.31,P=0.08).Correlation analysis found APOEε4( carrier or non-carrier) and mini mental state examination ( MMSE) score had no effects on BDNF levels ( P>0.05) .Conclusion Serum levels of BDNF could not be as a candidate peripheral marker for early diagnosis of AD .Serum levels of BDNF could not reflect the severity of AD .