CAJ | 학술논문

目的探讨糖原合成酶-3β(glycogen synthase kinase-3β,GSK-3β)在慢性脑缺血大鼠认知功能障碍和焦虑情绪变化中的作用.方法 51只雄性SD大鼠按随机数字表法随机分为假手术组(sham,n=17)、慢性脑缺血组(2VO,n=17)和慢性脑缺血+氯化锂组(2VO+LiCl,n=17).各组分别于第3,7,14,21,28 d腹腔注射LiCl或生理盐水5次处理后,建立脑缺血模型.于脑缺血28 d后进行空间学习记忆、恐惧记忆和焦虑情绪水平检测.结果水迷宫测试显示2VO组大鼠学习搜索发现平台的潜伏期明显长于sham组(第4天P＜0.01,第3,5,6,7天P＜0.05),2VO+LiCl组学习潜伏期显著短于2VO组(第4天P＜0.01,第5,6,7天P＜0.05),去掉平台后2VO组大鼠到达原目标位置的时间明显长于sham组(P＜0.05),2VO+LiCl组则明显短于2VO组(P＜0.01).电跳台测试显示2VO组大鼠潜伏期明显短于sham组[2VO组潜伏期:(41.00±1.87)s;sham组潜伏期:(44.55±2.77)s](P＜0.05),而2VO+LiCl组大鼠潜伏期明显短于2VO组[2VO+LiCl组潜伏期:(43.40±1.35)s](P＜0.05),2VO组犯错次数明显多于sham组(P＜0.05),2VO+LiCl组犯错次数明显少于2VO组(P＜0.05).高架十字迷宫试验显示2VO组大鼠开放臂滞留时间比例明显低于sham组[2VO组:(0.23±0.01);sham组:(0.25±0.01)](P＜0.01),而2VO+ LiCl组比例明显高于2VO组[2VO+ LiCl组:(0.24±0.01)](P＜0.05),2VO组大鼠入开放臂次数比例显著低于sham组(P＜0.05),而2VO+LiCl组比例明显高于2VO组(P＜0.05).结论慢性脑缺血引起大鼠学习记忆功能减退和焦虑情绪障碍,而GSK-3β抑制剂则可以明显改善慢性脑缺血引起的学习记忆能力下降和焦虑情绪障碍.
목적 탐토당원합성매-3β(glycogen synthase kinase-3β,GSK-3β)재만성뇌결혈대서인지공능장애화초필정서변화중적작용.방법 51지웅성SD대서안수궤수자표법수궤분위가수술조(sham,n=17)、만성뇌결혈조(2VO,n=17)화만성뇌결혈+록화리조(2VO+LiCl,n=17).각조분별우제3,7,14,21,28 d복강주사LiCl혹생리염수5차처리후,건립뇌결혈모형.우뇌결혈28 d후진행공간학습기억、공구기억화초필정서수평검측.결과 수미궁측시현시2VO조대서학습수색발현평태적잠복기명현장우sham조(제4천P＜0.01,제3,5,6,7천P＜0.05),2VO+LiCl조학습잠복기현저단우2VO조(제4천P＜0.01,제5,6,7천P＜0.05),거도평태후2VO조대서도체원목표위치적시간명현장우sham조(P＜0.05),2VO+LiCl조칙명현단우2VO조(P＜0.01).전도태측시현시2VO조대서잠복기명현단우sham조[2VO조잠복기:(41.00±1.87)s;sham조잠복기:(44.55±2.77)s](P＜0.05),이2VO+LiCl조대서잠복기명현단우2VO조[2VO+LiCl조잠복기:(43.40±1.35)s](P＜0.05),2VO조범착차수명현다우sham조(P＜0.05),2VO+LiCl조범착차수명현소우2VO조(P＜0.05).고가십자미궁시험현시2VO조대서개방비체류시간비례명현저우sham조[2VO조:(0.23±0.01);sham조:(0.25±0.01)](P＜0.01),이2VO+ LiCl조비례명현고우2VO조[2VO+ LiCl조:(0.24±0.01)](P＜0.05),2VO조대서입개방비차수비례현저저우sham조(P＜0.05),이2VO+LiCl조비례명현고우2VO조(P＜0.05).결론 만성뇌결혈인기대서학습기억공능감퇴화초필정서장애,이GSK-3β억제제칙가이명현개선만성뇌결혈인기적학습기억능력하강화초필정서장애.
Objective To explore the roles of glycogen synthase kinase-3β (GSK-3β) in cognitive dysfunction and emotional alterations after rat's chronic cerebral ischemia.Methods 51 SD rats were randomly divided into sham group (sham group,n=17),chronic cerebral ischemia group (2VO group,n=17),chronic cerebral ischemia group + LiCl group (2VO + LiCl group,n=17),according to the table of random number.All groups were intraperitoneally injected with LiCl or saline on 3rd,7th,14th,21 st and 28th day,and then produced the chronic cerebral ischemia models.On the 28th day after model,the spatial learning and memory,fear memory,and anxiety emotion were detected.Results The Morris water maze test showed that 2VO group spent longer latent time searching and finding the platform than sham group (4th day P＜0.01,3rd,5th,6th,7th day P＜0.05).2VO+LiCl group spent shorter latent time than 2VO group (4th day P＜0.01,5th,6th,7th day P＜0.05).After removing the platform,2VO group spent longer time arriving the former location than sham group (P＜0.05).And 2VO+LiCl group spent dramatically different time compared to 2VO group (P＜0.01).Step-down test showed 2VO group spent shorter latent time than sham group (2VO group:(41.00±1.87)s,sham group:(44.55±2.77)s) (P＜0.05).2VO+ LiCl group spent dramatically longer latent time compared to 2VO group (2VO +LiCl group:(43.40± 1.35)s) (P＜ 0.05).2VO group made much more mistakes times than sham group (P＜0.05).2VO+LiCl group made dramatically less mistakes times compared to 2VO group (P＜0.05).The elevated plus maze test showed 2VO group had much less ratio of retention time in open arms (among total arms retention time) than sham group (2VO group:(0.23± 0.01),sham group:(0.25± 0.01)) (P＜ 0.01).2VO + LiCl group had much larger ratio than 2VO group (2VO + LiCl group:(0.24±0.01),P＜0.05).2VO group had much less ratio of entry times in open arms (among total arms entry times) than sham group (P＜0.01).2VO+LiC1 group had much larger ratio than 2VO group(P＜0.05).Conclusion Chronic cerebral ischemia can lead to deterioration of learning and memory,and anxiety emotion for rats.However,inhibition of GSK-3β can ameliorate these alterations.