CAJ | 학술논문

本文初步探究不同浓度家蝇幼虫抗菌肽对FBL－3红白血病原代细胞增殖的影响。采用诱导、研磨、离心、 C18固相萃取等方法制备家蝇幼虫抗菌肽混合物，然后处理传代期的FBL－3细胞，调整浓度为2．25×106／mL并通过尾静脉注射C57BL／6小鼠，建立红白血病动物模型。待肿瘤生长较大时，处死并剥离肿瘤，再通过研磨、裂红液裂解、获取原代肿瘤细胞并培养。将抗菌肽混合物调整为不同浓度作用于传代期的FBL－3原代细胞，之后经倒置显微镜观察照相，台盼蓝染色试验计算死亡率、 CCK－8试剂盒做细胞毒性检测。结果显示抗菌肽混合物浓度为250～350μg／mL作用FBL－3原代细胞时，细胞碎片大量增多，死亡率增高，细胞毒性变大。初步证明了抗菌肽混合物在250～350μg／mL抑制细胞增殖效果明显，在300μg／mL浓度下抑制作用最强，本研究结果可作为动物模型体内实验的参考。
본문초보탐구불동농도가승유충항균태대FBL－3홍백혈병원대세포증식적영향。채용유도、연마、리심、 C18고상췌취등방법제비가승유충항균태혼합물，연후처리전대기적FBL－3세포，조정농도위2．25×106／mL병통과미정맥주사C57BL／6소서，건립홍백혈병동물모형。대종류생장교대시，처사병박리종류，재통과연마、렬홍액렬해、획취원대종류세포병배양。장항균태혼합물조정위불동농도작용우전대기적FBL－3원대세포，지후경도치현미경관찰조상，태반람염색시험계산사망솔、 CCK－8시제합주세포독성검측。결과현시항균태혼합물농도위250～350μg／mL작용FBL－3원대세포시，세포쇄편대량증다，사망솔증고，세포독성변대。초보증명료항균태혼합물재250～350μg／mL억제세포증식효과명현，재300μg／mL농도하억제작용최강，본연구결과가작위동물모형체내실험적삼고。
The inhibition effect of antimicrobial peptides from the housefly ( Musca domestica) larvae on primary cell FBL-3 was tested under laboratory.Antibacterial peptides extracts was purified with C18 solid phase methods from induced housefly larva.Erythroleukemia mice model was prepared by injected 2.25 ×106/mL FBL-3 cells into C57BL/6 mouse via the tail vein.The primary culture of FBL-3 cells was harvested from the mice and utilized to test the inhibition effect of the antibacterial peptide extracts.When observed with trypan blue staining methods, antibacterial peptide mixture at a concentration of 250-350 μg/mL affects FBL-3 primary cells significantly with increased cell debris and more mortality rate.Cell apoptosis was also shown in the concentration range tested with CCK-8 kit.The reference concentration of antibacterial peptide would be applied in further tests in vivo.