CAJ | 학술논문

目的探讨一重与三重脑震荡(cerebral concussion,CC)后不同时期强迫游泳实验(forced swimming test,FST)抑郁样行为结构变化特点.方法成年SD大鼠24只,用自制单摆闭合式脑损伤机械打击装置撞击大鼠额顶部,建立一重和三重CC大鼠模型,符合损伤判断标准的损伤大鼠被随机分为一次性脑震荡PCC组、及打击间隔分别为24 h与48 h的三重脑震荡3MCC 24 h组和3MCC 48 h组,另设一正常对照组,每组8只大鼠.应用FST行为学实验在伤后第3天、7天、15天、30天四个时间点进行大鼠损伤后抑郁样行为结构变化检测.结果 (1)与对照组相比,各损伤组大鼠的爬壁行为频数显著下降,均差异有统计学意义(P＜0.05),其中各损伤组下降最大的时间点是:PCC组在伤后30 d为(6.00±3.30)次,3MCC48 h组在伤后7d为(5.75±3.01)次,3MCC 24 h组在伤后7d为(3.25±1.28)次,且爬壁行为的频数按PCC组＞3MCC 48 h组＞3MCC 24 h组依次递减;(2)与对照组相比,3MCC 24 h和3MCC 48 h组大鼠的静止不动行为频数均明显上升,均差异有统计学意义(P＜0.05),其中各损伤组上升最大的时间点是:3MCC 48 h组在伤后7d为(19.00±2.83)次,3MCC 24 h组在伤后7d为(22.00±4.04)次,PCC组仅在伤后第30天差异有统计学意义,即(16.75±3.62)次,静止不动行为上升程度依次为PCC组＜3MCC 48 h组＜3MCC 24 h组.(3)游泳行为在各组间比较均差异无统计学意义(P＞0.05).结论一重与三重脑震荡大鼠损伤后出现不同程度的抑郁样行为,且三重CC重于一重CC、打击间隔短的重于打击间隔长的三重CC.
목적 탐토일중여삼중뇌진탕(cerebral concussion,CC)후불동시기강박유영실험(forced swimming test,FST)억욱양행위결구변화특점.방법 성년SD대서24지,용자제단파폐합식뇌손상궤계타격장치당격대서액정부,건립일중화삼중CC대서모형,부합손상판단표준적손상대서피수궤분위일차성뇌진탕PCC조、급타격간격분별위24 h여48 h적삼중뇌진탕3MCC 24 h조화3MCC 48 h조,령설일정상대조조,매조8지대서.응용FST행위학실험재상후제3천、7천、15천、30천사개시간점진행대서손상후억욱양행위결구변화검측.결과 (1)여대조조상비,각손상조대서적파벽행위빈수현저하강,균차이유통계학의의(P＜0.05),기중각손상조하강최대적시간점시:PCC조재상후30 d위(6.00±3.30)차,3MCC48 h조재상후7d위(5.75±3.01)차,3MCC 24 h조재상후7d위(3.25±1.28)차,차파벽행위적빈수안PCC조＞3MCC 48 h조＞3MCC 24 h조의차체감;(2)여대조조상비,3MCC 24 h화3MCC 48 h조대서적정지불동행위빈수균명현상승,균차이유통계학의의(P＜0.05),기중각손상조상승최대적시간점시:3MCC 48 h조재상후7d위(19.00±2.83)차,3MCC 24 h조재상후7d위(22.00±4.04)차,PCC조부재상후제30천차이유통계학의의,즉(16.75±3.62)차,정지불동행위상승정도의차위PCC조＜3MCC 48 h조＜3MCC 24 h조.(3)유영행위재각조간비교균차이무통계학의의(P＞0.05).결론 일중여삼중뇌진탕대서손상후출현불동정도적억욱양행위,차삼중CC중우일중CC、타격간격단적중우타격간격장적삼중CC.
Objective To observe the changes of depress-like behavior characteristics in different period of the forced swimming test(FST) after pure cerebral concussion(PCC) and multiple cerebral concussion(3MCC) model rats.Methods 24 adult healthy male SD rats were chosen.A brain concussion model was produced in rats using a simple pendulum device,according to the impacting times (one or three times) and impacting interval (24 h or 48 h).The rats were randomly assigned to the one time PCC group,triple MCC 24 h group(3MCC 24 h) and triple MCC 48 h group(3MCC 48 h),and one normal control group was used(8 rats in each group).FST was used to assess the change of depress-like behavior characteristics in rats.Results The following depress-like behavior changes of FST (climbing;swimming;motionless) were observed at the 3rd,7th,15th,30th day after injury were as follows:(1) Compared with the normal control group,the decline of "climbing" behavior frequency had statistically significant (P＜0.05)in each injury group,the maximum decreased time in each injury group:(6.00 ±3.30)times after 30 d injury in PCC group,(3.25±1.28) times after 7 d injury in 3MCC 24 h group,(5.75± 3.01)times after 7 d injury in 3MCC 48 h group.Among all three injuried groups,the characteristic of "climbing" behavior had an decrease progressively as PCC group＜3MCC 48 h group＜3MCC 24 h group.(2)Compared with the normal control group,the increase of "motionless" behavior frequency had statistically significant(P＜0.05)in both 3MCC injuried group at all four test points post-injury,the maximum increased time in each injury group:(19.00±2.83) times after 7 d injury in 3MCC 48 h group,(22.00±4.04) times after 7 d injury in 3MCC 24h group.But the PCC group had statistically significant (P＜ 0.05) only at the 30th day after injury,it was (16.75± 3.62) times.Among three injury groups,the characteristic of "motionless" behavior had an increase progressively as PCC group ＜3MCC 48 h group＜3MCC 24 h group.(3)The changes of " swimming" behavior between each group has no statistically significant(P＞0.05).Conclusion There are some depress-like behaviors after one time CC and triple CC in rats,while the severity of triple CC is more severe than one time CC,and the short time interval strike triple CC is more severe than the long time interval triple CC.