中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2015年
5期
393-396
,共4页
许慧%李光武%徐金勇%刘扬
許慧%李光武%徐金勇%劉颺
허혜%리광무%서금용%류양
香兰素%嗅觉通路%抑郁症%脑源性神经营养因子
香蘭素%嗅覺通路%抑鬱癥%腦源性神經營養因子
향란소%후각통로%억욱증%뇌원성신경영양인자
Vanillin%Olfactory pathway%Depressive disorder%Brain derived neurotrophic factor
目的 研究香兰素吸嗅对抑郁症模型大鼠抑郁样行为的影响,评价其对抑郁症的干预作用,并探讨其可能的作用机制.方法 将80只SD雄性大鼠,采用慢性不可预见性中等强度应激(CUMS)或者嗅球毁损,制备抑郁样模型,符合模型大鼠随机分为香兰素组、氟西汀组、模型组、嗅球毁损+香兰素组及假手术组,给予相应处理5周,评价大鼠在干预后各时间点的神经行为学变化及脑源性神经营养因子(BD-NF)含量及其在海马CA3区的表达.结果 (1)行为学结果:干预2周后强迫游泳实验香兰素组大鼠不动时间[(12.78±7.50)s],显著低于模型组[(57.33±32.16)s],差异有统计学意义(P＜0.05);24 h糖水消耗量实验香兰素组[(52.88±25.18)g]显著高于模型组[(37.40± 19.33)g],差异有统计学意义(P＜0.05);(2)ELISA结果显示:脑组织匀浆液BDNF与总蛋白(Pr)含量比值香兰素组(0.54±0.13),显著高于模型组(0.36±0.06)和OBX组(0.40±0.06),差异均有统计学意义(P＜0.01);(3)免疫组织化学结果显示:香兰素组海马CA3区BDNF的表达明显增加,图像分析平均光密度值香兰素组(0.40±0.03)显著高于模型组(0.25±0.04)和OBX组(0.28±0.03),均差异有统计学意义(P＜0.01).结论 香兰素吸嗅可以缓解抑郁模型大鼠的抑郁样行为,其机制可能是提高脑内BDNF含量,增加海马神经发生,进而调节脑区功能,从而达到抑郁症防治的作用.
目的 研究香蘭素吸嗅對抑鬱癥模型大鼠抑鬱樣行為的影響,評價其對抑鬱癥的榦預作用,併探討其可能的作用機製.方法 將80隻SD雄性大鼠,採用慢性不可預見性中等彊度應激(CUMS)或者嗅毬燬損,製備抑鬱樣模型,符閤模型大鼠隨機分為香蘭素組、氟西汀組、模型組、嗅毬燬損+香蘭素組及假手術組,給予相應處理5週,評價大鼠在榦預後各時間點的神經行為學變化及腦源性神經營養因子(BD-NF)含量及其在海馬CA3區的錶達.結果 (1)行為學結果:榦預2週後彊迫遊泳實驗香蘭素組大鼠不動時間[(12.78±7.50)s],顯著低于模型組[(57.33±32.16)s],差異有統計學意義(P＜0.05);24 h糖水消耗量實驗香蘭素組[(52.88±25.18)g]顯著高于模型組[(37.40± 19.33)g],差異有統計學意義(P＜0.05);(2)ELISA結果顯示:腦組織勻漿液BDNF與總蛋白(Pr)含量比值香蘭素組(0.54±0.13),顯著高于模型組(0.36±0.06)和OBX組(0.40±0.06),差異均有統計學意義(P＜0.01);(3)免疫組織化學結果顯示:香蘭素組海馬CA3區BDNF的錶達明顯增加,圖像分析平均光密度值香蘭素組(0.40±0.03)顯著高于模型組(0.25±0.04)和OBX組(0.28±0.03),均差異有統計學意義(P＜0.01).結論 香蘭素吸嗅可以緩解抑鬱模型大鼠的抑鬱樣行為,其機製可能是提高腦內BDNF含量,增加海馬神經髮生,進而調節腦區功能,從而達到抑鬱癥防治的作用.
목적 연구향란소흡후대억욱증모형대서억욱양행위적영향,평개기대억욱증적간예작용,병탐토기가능적작용궤제.방법 장80지SD웅성대서,채용만성불가예견성중등강도응격(CUMS)혹자후구훼손,제비억욱양모형,부합모형대서수궤분위향란소조、불서정조、모형조、후구훼손+향란소조급가수술조,급여상응처리5주,평개대서재간예후각시간점적신경행위학변화급뇌원성신경영양인자(BD-NF)함량급기재해마CA3구적표체.결과 (1)행위학결과:간예2주후강박유영실험향란소조대서불동시간[(12.78±7.50)s],현저저우모형조[(57.33±32.16)s],차이유통계학의의(P＜0.05);24 h당수소모량실험향란소조[(52.88±25.18)g]현저고우모형조[(37.40± 19.33)g],차이유통계학의의(P＜0.05);(2)ELISA결과현시:뇌조직균장액BDNF여총단백(Pr)함량비치향란소조(0.54±0.13),현저고우모형조(0.36±0.06)화OBX조(0.40±0.06),차이균유통계학의의(P＜0.01);(3)면역조직화학결과현시:향란소조해마CA3구BDNF적표체명현증가,도상분석평균광밀도치향란소조(0.40±0.03)현저고우모형조(0.25±0.04)화OBX조(0.28±0.03),균차이유통계학의의(P＜0.01).결론 향란소흡후가이완해억욱모형대서적억욱양행위,기궤제가능시제고뇌내BDNF함량,증가해마신경발생,진이조절뇌구공능,종이체도억욱증방치적작용.
Objective To evaluate the relieving effects of vanillin sinffing on depression-like behaviors in depressed rats and to explore the possible underlying mechanism.Methods Depression animal model established by chronic unpredictable medium intensity stress combined with isolation and destroy the olfactory bulb.The depressed rats were divided randomly into vanillin inhalation group,fluoxetine hydrochloride group,depression model group,olfactory bulbectomy with the vanillin inhalation treatment group and sham-operated group.Nervous behavioral changes had been observed at different time after the administration of 5 weeks.The concentration of brain derived neurotrophic factor(BDNF) in the brain homogenate and the positive expression of BDNF in hippocampus had been also measured.Results Two weeks after the intervention,the immobility time of vanillin group((12.78 ±7.50) s) was lower than that of the model group((57.33±32.16) s) (P＜0.05).The consumption of saccharose in vanillin group((52.88±25.18)g) was higher than that of model group((37.40±19.33) g) (P＜0.05).BDNF of the brain homogenate in vanillin group (0.54±0.13) was significantly increased compared with model group (0.36± 0.06) (P＜0.01).When compared with the OBX group (0.40±0.06),similar result was obtained.Immunohistochemistry and the average density of image analysis revealed that the expression of BNDF of hippocampal CA3 in vanillin group (0.40±0.03)was significantly increased compared with model group (0.25±0.04) and OBX group (0.28±0.03) (P＜0.01).Conclusion Vanillin inhalation significantly relieves depression-like behaviors in depression rats.The possible mechanism may increase hippocampal neurogenesis by raising brain derived neurotrophic factor in brain.
