中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2015年
5期
402-406
,共5页
谷景阳%韩金红%詹和琴%王长虹%刘聪%单筱雯%翟飞
穀景暘%韓金紅%詹和琴%王長虹%劉聰%單篠雯%翟飛
곡경양%한금홍%첨화금%왕장홍%류총%단소문%적비
抑郁%髓鞘碱性蛋白%丰富环境%氟西汀%海马%前额叶
抑鬱%髓鞘堿性蛋白%豐富環境%氟西汀%海馬%前額葉
억욱%수초감성단백%봉부배경%불서정%해마%전액협
Depression%Myelin basic protein%Enriched environment%Fluoxetine%Hippocampus%Prefrontal
目的 探讨丰富环境联合氟西汀干预对慢性应激所致抑郁大鼠行为学的影响,以及海马区和前额叶区髓鞘碱性蛋白(myelin basic protein,MBP)变化的影响.方法 将50只成年雄性SD大鼠随机分为对照组、氟西汀组、模型组、丰富环境(enriched environment,EE)组和EE+氟西汀组,后四组在第1～3周接受慢性不可预见性刺激(chronic unpredicted mild stress,CUMS)刺激;第4～6周,氟西汀组、EE组以及EE+氟西汀组大鼠接受EE和(或)氟西汀干预;采用蔗糖水消耗实验、旷场实验、体质量变化评估大鼠的行为学变化;使用免疫组化法测量大鼠海马各亚区以及前额叶区MBP的含量.结果 第3周末,应激刺激大鼠的各项行为学评估较对照组明显下降,差异有统计学意义(P＜0.05);第6周末,接受EE和(或)氟西汀干预后的应激大鼠抑郁样行为显著改善,模型组大鼠海马CA1、DG区以及前额叶区MBP含量较对照组差异均有统计学意义(平均光密度:模型组依次为0.199±0.024,0.204±0.021,0.225±0.028;对照组依次为0.279±0.034,0.288±0.043,0.308±0.053,P＜0.05);氟西汀组、EE组以及EE+氟西汀组大鼠海马CA1、DG区以及前额叶区的MBP含量较模型组明显升高,差异均有统计学意义(氟西汀组依次为0.259±0.047,0.266±0.052,0.284±0.031;EE组依次为0.257±0.038,0.258±0.042,0.286±0.037;EE+氟西汀组依次为0.271±0.046,0.279±0.040,0.289±0.041,P＜0.05).结论 CUMS所致大鼠抑郁样行为的出现与海马CA1、DG区及前额叶区MBP含量的变化有关,氟西汀及EE干预均能逆转大鼠的抑郁样行为,且可使MBP含量恢复正常.
目的 探討豐富環境聯閤氟西汀榦預對慢性應激所緻抑鬱大鼠行為學的影響,以及海馬區和前額葉區髓鞘堿性蛋白(myelin basic protein,MBP)變化的影響.方法 將50隻成年雄性SD大鼠隨機分為對照組、氟西汀組、模型組、豐富環境(enriched environment,EE)組和EE+氟西汀組,後四組在第1～3週接受慢性不可預見性刺激(chronic unpredicted mild stress,CUMS)刺激;第4～6週,氟西汀組、EE組以及EE+氟西汀組大鼠接受EE和(或)氟西汀榦預;採用蔗糖水消耗實驗、曠場實驗、體質量變化評估大鼠的行為學變化;使用免疫組化法測量大鼠海馬各亞區以及前額葉區MBP的含量.結果 第3週末,應激刺激大鼠的各項行為學評估較對照組明顯下降,差異有統計學意義(P＜0.05);第6週末,接受EE和(或)氟西汀榦預後的應激大鼠抑鬱樣行為顯著改善,模型組大鼠海馬CA1、DG區以及前額葉區MBP含量較對照組差異均有統計學意義(平均光密度:模型組依次為0.199±0.024,0.204±0.021,0.225±0.028;對照組依次為0.279±0.034,0.288±0.043,0.308±0.053,P＜0.05);氟西汀組、EE組以及EE+氟西汀組大鼠海馬CA1、DG區以及前額葉區的MBP含量較模型組明顯升高,差異均有統計學意義(氟西汀組依次為0.259±0.047,0.266±0.052,0.284±0.031;EE組依次為0.257±0.038,0.258±0.042,0.286±0.037;EE+氟西汀組依次為0.271±0.046,0.279±0.040,0.289±0.041,P＜0.05).結論 CUMS所緻大鼠抑鬱樣行為的齣現與海馬CA1、DG區及前額葉區MBP含量的變化有關,氟西汀及EE榦預均能逆轉大鼠的抑鬱樣行為,且可使MBP含量恢複正常.
목적 탐토봉부배경연합불서정간예대만성응격소치억욱대서행위학적영향,이급해마구화전액협구수초감성단백(myelin basic protein,MBP)변화적영향.방법 장50지성년웅성SD대서수궤분위대조조、불서정조、모형조、봉부배경(enriched environment,EE)조화EE+불서정조,후사조재제1～3주접수만성불가예견성자격(chronic unpredicted mild stress,CUMS)자격;제4～6주,불서정조、EE조이급EE+불서정조대서접수EE화(혹)불서정간예;채용자당수소모실험、광장실험、체질량변화평고대서적행위학변화;사용면역조화법측량대서해마각아구이급전액협구MBP적함량.결과 제3주말,응격자격대서적각항행위학평고교대조조명현하강,차이유통계학의의(P＜0.05);제6주말,접수EE화(혹)불서정간예후적응격대서억욱양행위현저개선,모형조대서해마CA1、DG구이급전액협구MBP함량교대조조차이균유통계학의의(평균광밀도:모형조의차위0.199±0.024,0.204±0.021,0.225±0.028;대조조의차위0.279±0.034,0.288±0.043,0.308±0.053,P＜0.05);불서정조、EE조이급EE+불서정조대서해마CA1、DG구이급전액협구적MBP함량교모형조명현승고,차이균유통계학의의(불서정조의차위0.259±0.047,0.266±0.052,0.284±0.031;EE조의차위0.257±0.038,0.258±0.042,0.286±0.037;EE+불서정조의차위0.271±0.046,0.279±0.040,0.289±0.041,P＜0.05).결론 CUMS소치대서억욱양행위적출현여해마CA1、DG구급전액협구MBP함량적변화유관,불서정급EE간예균능역전대서적억욱양행위,차가사MBP함량회복정상.
Objective To investigate the effects of intervention with the fluoxetine and the enriched environment on chronic stress induced depression behavior of rats,and the changes of myelin basic protein in hippocampus and prefrontal regions.Methods 50 adult male SD rats were randomly divided into control group,fluoxetine group,model group,enriched environment (EE) group and EE plus fluoxetine group.Fluoxetine group,model group,EE group and EE plus fluoxetine group underwent chronic unpredictable stress stimulus in the first to third week,and fluoxetine group,EE group,EE plus fluoxetine group underwent the intervention with EE and (or) fluoxetine in the fourth to sixth week.The changes of behavior in rats were evaluated by sucrose water consumption,open field test and weight changes.The content of MBP in each subregion of hippocampus and prefrontal regions of rats was measured with immunocytochemical methods.Results At the third weekend,the assessed behaviors of stressed rats decreased significantly compared with control group (P＜0.05);and at the sixth weekend,the behaviors of stressed rats restored after treated with EE and (or) fluoxetine.The content of MBP in the rat hippocampus CA1,DG area and prefrontal area of model group declined clearly compared with control group (mean density of model group orderly:0.199±0.024,0.204±0.021,0.225±0.028;control group orderly:0.279±0.034,0.288±0.043,0.308±0.053,P＜0.05).The content of MBP in the rat of fluoxetine group,EE group and EE plus fluoxetine group increased obviously compared with model group (fluoxetine group orderly:0.259± 0.047,0.266± 0.052,0.284 ± 0.031;EE group orderly:0.257±0.038,0.258±0.042,0.286±0.037;EE plus fluoxetine group orderly:0.271± 0.046,0.279±0.040,0.289±0.041,P＜0.05).Conclusion The depression-like behavior of rats induced by chronic unpredictable stress is associated with the change of the content of MBP in hippocampal CA1,DG area and prefrontal area;and the depression-like behavior and the content of MBP decrease are reversed after the intervention with fluoxetine and EE.
