中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2015年
5期
407-410
,共4页
王永志%韩玉%李宝金%李杨帆%杜仪%赵静洁%李丽
王永誌%韓玉%李寶金%李楊帆%杜儀%趙靜潔%李麗
왕영지%한옥%리보금%리양범%두의%조정길%리려
柴胡疏肝散%抑郁症%5-羟色胺%色氨酸羟化酶2
柴鬍疏肝散%抑鬱癥%5-羥色胺%色氨痠羥化酶2
시호소간산%억욱증%5-간색알%색안산간화매2
Chaihu Shugan Powder%Depression%5-hydroxytryptamine%Tryptophan hydroxylase 2
目的 观察柴胡疏肝散对抑郁大鼠海马神经递质5-羟色胺(5-HT)含量及中缝核色氨酸羟化酶2(TPH2)表达量的影响.方法 雄性SD大鼠60只,采用随机数字表法分为正常组、利血平模型组、盐酸氟西汀组、柴胡疏肝散低剂组和高剂组,每组12只.小剂量腹腔注射利血平制备抑郁模型大鼠,实验期间对大鼠体质量、行为学改变进行观察,ELISA法测定海马组织单胺类神经递质5-HT含量,Western blot法检测大鼠脑中缝核TPH2表达量.结果 与模型组体质量[(225.02±5.23)g]比较,柴胡疏肝散高剂组体质量[(238.78±5.16)g]增加明显(P＜0.01);与模型组Open-Field行为学得分[(12.6±5.1)分]比较,柴胡疏肝散高剂组可以改善抑郁大鼠行为[(15.6±7.8)分,P＜0.05].与模型组大鼠脑内5-HT含量[(1.09±0.27)ng/ml]和TPH2表达量[(0.16±0.04)]比较,高剂量柴胡疏肝散对两者的提高十分明显[分别为(1.80±0.58) ng/ml,(0.66±0.21)],差异有统计学意义(P＜0.01).结论 中药柴胡疏肝散抗抑郁效果显著,可能与增加大鼠海马组织5-HT含量、提高脑中缝核TPH2表达量有关.
目的 觀察柴鬍疏肝散對抑鬱大鼠海馬神經遞質5-羥色胺(5-HT)含量及中縫覈色氨痠羥化酶2(TPH2)錶達量的影響.方法 雄性SD大鼠60隻,採用隨機數字錶法分為正常組、利血平模型組、鹽痠氟西汀組、柴鬍疏肝散低劑組和高劑組,每組12隻.小劑量腹腔註射利血平製備抑鬱模型大鼠,實驗期間對大鼠體質量、行為學改變進行觀察,ELISA法測定海馬組織單胺類神經遞質5-HT含量,Western blot法檢測大鼠腦中縫覈TPH2錶達量.結果 與模型組體質量[(225.02±5.23)g]比較,柴鬍疏肝散高劑組體質量[(238.78±5.16)g]增加明顯(P＜0.01);與模型組Open-Field行為學得分[(12.6±5.1)分]比較,柴鬍疏肝散高劑組可以改善抑鬱大鼠行為[(15.6±7.8)分,P＜0.05].與模型組大鼠腦內5-HT含量[(1.09±0.27)ng/ml]和TPH2錶達量[(0.16±0.04)]比較,高劑量柴鬍疏肝散對兩者的提高十分明顯[分彆為(1.80±0.58) ng/ml,(0.66±0.21)],差異有統計學意義(P＜0.01).結論 中藥柴鬍疏肝散抗抑鬱效果顯著,可能與增加大鼠海馬組織5-HT含量、提高腦中縫覈TPH2錶達量有關.
목적 관찰시호소간산대억욱대서해마신경체질5-간색알(5-HT)함량급중봉핵색안산간화매2(TPH2)표체량적영향.방법 웅성SD대서60지,채용수궤수자표법분위정상조、리혈평모형조、염산불서정조、시호소간산저제조화고제조,매조12지.소제량복강주사리혈평제비억욱모형대서,실험기간대대서체질량、행위학개변진행관찰,ELISA법측정해마조직단알류신경체질5-HT함량,Western blot법검측대서뇌중봉핵TPH2표체량.결과 여모형조체질량[(225.02±5.23)g]비교,시호소간산고제조체질량[(238.78±5.16)g]증가명현(P＜0.01);여모형조Open-Field행위학득분[(12.6±5.1)분]비교,시호소간산고제조가이개선억욱대서행위[(15.6±7.8)분,P＜0.05].여모형조대서뇌내5-HT함량[(1.09±0.27)ng/ml]화TPH2표체량[(0.16±0.04)]비교,고제량시호소간산대량자적제고십분명현[분별위(1.80±0.58) ng/ml,(0.66±0.21)],차이유통계학의의(P＜0.01).결론 중약시호소간산항억욱효과현저,가능여증가대서해마조직5-HT함량、제고뇌중봉핵TPH2표체량유관.
Objective To explore the effect of Chaihu Shugan Powder (CSP) on the content of 5-hydroxytryptamine (5-HT) in the hippocampus and the expression of tryptophan hydroxylase 2(TPH2) in median raphe nuclei in depression rats.Methods SD rats were randomly divided into normal group(n=12),model group (n=12),fluoxetine group (n=12),low-dose and high-dose CSP group (n =12,respectively).Depression model was made by reserpine intraperitoneal injection.During the experiment,the weight and the open-field scores were calculated;the content of 5-HT was detected by ELISA.The expression of TPH2 in median raphe nuclei was detected by Western blot.Results Compared with the weight ((225.02±5.23) g),the open-field scores ((12.6± 5.1)score) and content of 5-HT ((1.09±0.27) ng/ml) in the model group,high-dose CSP showed significantly improve the depressive rats in weight,open field score and content of 5-HT ((238.78±5.16) g,(15.6±7.8) score and (1.80±0.58) ng/ml,respectively;P＜0.05 or P＜0.01).The expression of TPH2 (0.66±0.21) in median raphe nuclei in the high-dose CSP group was apparently increased compared with that in the model group(0.16±0.04) (P ＜0.01).Conclusion CSP have the effects of anti-depression,which could be related with the increase of the 5-HT content in the hippocampus and the expression of TPH2 in median raphe nuclei.
