CAJ | 학술논문

目的探讨不同剂量5-羟色胺2A/2C受体激动剂与5-羟色胺2A受体拮抗剂对慢性间歇低氧(CIH)大鼠颏舌肌放电活性长时程易化(LTF)的影响.方法成年雄性SD大鼠30只,体重180～200g,通过随机数字表法将大鼠分为常氧对照组(A组,5只)和慢性间歇低氧组(CIH组,25只),其中CIH组分成5个亚组,分别为生理盐水组(B组)、5-羟色胺2A/2C受体激动剂30 μg组(C组)、5-羟色胺2A/2C受体激动剂50 μg组(D组)、5-羟色胺2A受体拮抗剂10 μg(E组)、5-羟色胺2A受体拮抗剂70 μg组(F组),每组各5只.CIH组大鼠放置于常压间隙低氧动物仓中,常氧组放置于常压常氧动物仓中,每天8h,连续处理4周,随后给予急性间歇低氧刺激,每次5 min,共3次,记录和比较各组大鼠给药前后颏舌肌肌电活性的变化.结果 B组大鼠颏舌肌放电活性明显高于A组.A组与B组在60 min时的放电幅度分别为(25.38±0.89)和(42.29±1.78)μV,差异有统计学意义(P＜0.01).与B组比较,C组和D组颏舌肌的放电幅度先上升后下降,上升幅度的差异具有统计学意义(P＜0.05).与B组比较,E组对颏舌肌的放电幅度影响不明显,差异无统计学意义(P＞0.05);但给予5-羟色胺2A受体拮抗剂70 μg后(F组),颏舌肌的放电幅度先下降后逐渐恢复至基础状态时的水平,下降幅度的差异具有统计学意义(P＜0.01).结论 CIH能够诱导颏舌肌LTF,5-羟色胺2A/2C受体激动剂可增强CIH大鼠颏舌肌放电活性,而5-羟色胺2A受体拮抗剂可降低CIH大鼠颏舌肌放电活性,二者均存在量效关系.
목적 탐토불동제량5-간색알2A/2C수체격동제여5-간색알2A수체길항제대만성간헐저양(CIH)대서해설기방전활성장시정역화(LTF)적영향.방법 성년웅성SD대서30지,체중180～200g,통과수궤수자표법장대서분위상양대조조(A조,5지)화만성간헐저양조(CIH조,25지),기중CIH조분성5개아조,분별위생리염수조(B조)、5-간색알2A/2C수체격동제30 μg조(C조)、5-간색알2A/2C수체격동제50 μg조(D조)、5-간색알2A수체길항제10 μg(E조)、5-간색알2A수체길항제70 μg조(F조),매조각5지.CIH조대서방치우상압간극저양동물창중,상양조방치우상압상양동물창중,매천8h,련속처리4주,수후급여급성간헐저양자격,매차5 min,공3차,기록화비교각조대서급약전후해설기기전활성적변화.결과 B조대서해설기방전활성명현고우A조.A조여B조재60 min시적방전폭도분별위(25.38±0.89)화(42.29±1.78)μV,차이유통계학의의(P＜0.01).여B조비교,C조화D조해설기적방전폭도선상승후하강,상승폭도적차이구유통계학의의(P＜0.05).여B조비교,E조대해설기적방전폭도영향불명현,차이무통계학의의(P＞0.05);단급여5-간색알2A수체길항제70 μg후(F조),해설기적방전폭도선하강후축점회복지기출상태시적수평,하강폭도적차이구유통계학의의(P＜0.01).결론 CIH능구유도해설기LTF,5-간색알2A/2C수체격동제가증강CIH대서해설기방전활성,이5-간색알2A수체길항제가강저CIH대서해설기방전활성,이자균존재량효관계.
Objective To explore the effects of different doses of 5-HT2A/2CR agonist or 5-HT2AR antagonist on genioglossus discharge in chronic intermittent hypoxia rats.Methods Thirty adult male Sprague-Dawley rats,each 180-200 g,were randomly divided into the normoxia group (group A,n =5) and the chronic intermittent hypoxia group (CIH group,n =25) by the random number table.The rats in the CIH group were fed in the intermittent hypoxia animal chambers,while the normoxia control group was placed in the normoxia animal chambers for 8 h per day for 4 consecutive weeks.After 28 days,5 rin × 3 times of stimulation with acute intermittent hypoxia (AIH) were given and the genioglossus muscle activity were then recorded and compared before and after intravenous injection of saline (group B,n =5),the 5-HT2A/2CR agonist 30 μg/3 μl (group C,n =5) or 50 μg/5 μl group (group D,n =5),5-HT2AR antagonist 10 μg/1 μl (group E,n =5) or 70 μg/7 μl (group F,n =5).Results The average peak amplitude in 60 min of genioglossus muscle activity in the CIH saline control group (group B) was significantly higher than that in the normo0ia group (group A),while the genioglossus discharge in the 2 groups were (42.29 ± 1.78) μV,(25.38±0.89) μV respectively(P ＜0.01).The 5-HT2A/2C R agonist (30 0g/3 μl or 50 μg/5 0l) caused the amplitude of genioglossus muscle activity firstly to increase and then to decrease,with the peak changes showing statistical difference (P ＜ 0.05).10 μg/1 μl 5-HT2A R antagonist did not influence genioglossus muscle activity (P ＞ 0.05),however,after intravenous injection of 70 μg/7 0l 5-HT2A R antagonist,the amplitude of genioglossus discharge firstly decreased and then gradually returned to baseline level,the difference being statistically significant,P ＜ 0.01.Conclusion Our results indicate that CIH strengthens the genioglossus discharge in CIH rats.The 5-HT2A/2C R agonist can strengthen the genioglossus discharge but the 5-HT2A R antagonists can weaken the effect,both of which have dose-related effects.