CAJ | 학술논문

【目的】观察电离辐射对血管内皮细胞增殖和纤维肌动蛋白（F‐actin ）的影响，为探索颌骨放射损伤机制及其预防和治疗提供参考。【方法】对人脐静脉血管内皮细胞株分别予以0 Gy、4 Gy、8 Gy、12 Gy、16 Gy剂量单次照射，照射后立即转入37℃培养箱继续培养12 h、24 h、48 h ，采用酶联免疫检测仪检测细胞增殖情况，流式细胞仪检测细胞F‐actin的含量。【结果】不同剂量单次照射各组照射后细胞生长速度明显减慢，且与剂量呈负相关，剂量高于12 Gy时细胞生长几乎完全抑制；同时，细胞F‐actin的含量急剧下降，且呈剂量和时间依赖关系。24 h后F‐actin有所回升，但仍明显低于辐射前。【结论】电离辐射可降低血管内皮细胞中的F‐actin ，破坏细胞骨架，抑制细胞的增殖。
【목적】관찰전리복사대혈관내피세포증식화섬유기동단백（F‐actin ）적영향，위탐색합골방사손상궤제급기예방화치료제공삼고。【방법】대인제정맥혈관내피세포주분별여이0 Gy、4 Gy、8 Gy、12 Gy、16 Gy제량단차조사，조사후립즉전입37℃배양상계속배양12 h、24 h、48 h ，채용매련면역검측의검측세포증식정황，류식세포의검측세포F‐actin적함량。【결과】불동제량단차조사각조조사후세포생장속도명현감만，차여제량정부상관，제량고우12 Gy시세포생장궤호완전억제；동시，세포F‐actin적함량급극하강，차정제량화시간의뢰관계。24 h후F‐actin유소회승，단잉명현저우복사전。【결론】전리복사가강저혈관내피세포중적F‐actin ，파배세포골가，억제세포적증식。
[Objective] To explore the mechanisms of radioactive damage of jaw ,provide rationales for its pre‐vention and treatment and examine the effects of ionizing radiation on the proliferation of vascular endothelial cell and F‐actin .[Methods] The vascular endothelial cells were irradiated with 0 ,4 ,8 ,12 ,16Gy 60Coγ‐ray and then culti‐vated in a 37℃ incubator for 12 ,24 ,48 h .Cell proliferation was detected with methyl thiazolyl tetrazolium (MTT) Cell Proliferation Assay and F‐actin by flow cytometry .[Results]The cell growth velocity of all groups slowed down distinctly after irradiation and showed an inverse correlation with dose .The cell growth was almost inhibited com‐pletely when the dose surpassed 12 Gy .F‐actins decreased after irradiation with doses and times ,and then recovered 24hs later but were lower than that before irradiation .[Conclusion] Ionizing irradiation may destroy the cytomem‐brane skeleton by decreasing F‐actin and inhibiting cell proliferation .