CAJ | 학술논문

目的采用生物信息学方法预测has-miR-26b、has-miR-1972、has-miR-4485、has-miR-4498和has-miR-4743的靶基因,并分析其所参与的生物学过程及信号通路,为后续功能研究提供理论依据.方法应用TargetScan、miRBD及DIANA-microT-CDS预测上述5个miRNA的靶基因,各个miRNA的预测结果分别取交集后,再取其合集,应用FunNet进行功能富集分析和信号通路富集分析.结果用3个在线数据库得到5个miRNA的靶基因的合集为734个;靶基因所涉及的生物学过程广泛,包括多项与中枢神经系统相关的条目,如皮层发育、轴突导向和延伸、突触传递以及学习和记忆过程等(P＜0.05);所涉及的信号通路包括轴突导向、谷氨酸能突触、Wnt信号通路、ErbB信号通路、mTOR信号通路、VEGF信号通路等与重症抑郁障碍存在密切联系;has-miR-26b、has-miR-1972、has-miR-4498等3个miRNA在重症抑郁障碍中可能起着更加重要的作用.结论 has-miR-26b、has-miR-1972、has-miR-4485、has-miR-4498和has-miR-4743可能与重症抑郁障碍的发病机制密切相关.
목적 채용생물신식학방법예측has-miR-26b、has-miR-1972、has-miR-4485、has-miR-4498화has-miR-4743적파기인,병분석기소삼여적생물학과정급신호통로,위후속공능연구제공이론의거.방법 응용TargetScan、miRBD급DIANA-microT-CDS예측상술5개miRNA적파기인,각개miRNA적예측결과분별취교집후,재취기합집,응용FunNet진행공능부집분석화신호통로부집분석.결과 용3개재선수거고득도5개miRNA적파기인적합집위734개;파기인소섭급적생물학과정엄범,포괄다항여중추신경계통상관적조목,여피층발육、축돌도향화연신、돌촉전체이급학습화기억과정등(P＜0.05);소섭급적신호통로포괄축돌도향、곡안산능돌촉、Wnt신호통로、ErbB신호통로、mTOR신호통로、VEGF신호통로등여중증억욱장애존재밀절련계;has-miR-26b、has-miR-1972、has-miR-4498등3개miRNA재중증억욱장애중가능기착경가중요적작용.결론 has-miR-26b、has-miR-1972、has-miR-4485、has-miR-4498화has-miR-4743가능여중증억욱장애적발병궤제밀절상관.
Objective To predict the target genes and function of has-miR-26b,has-miR-1972,has-miR4485,has-miR-4498,and has-miR-4743 by bioinformatics analysis,and provide the theoretical basis for the further research.Methods The targets of the five microRNAs were predicted by Target Scan,miRBD,and DIANA-microT-CDS,and the result were analyzed by gene ontology and pathway analysis using FunNet.Results 734 predicted targets were obtained by finding the intersected genes of Target Scan,miRBD,and DIANA-microT-CDS.GO analysis showed that biological processes regulated by the differentially expressed microRNAs included diverse terms,among which some terms (e.g.,central nervous system development,neuron differentiation,axonogenesis,synaptic transmission,learning,and memory,etc.) had direct relationship with the central nervous system and brain functions.The pathway analysis showed that a significant enrichment in several pathways related to neuronal brain function,such as axon guidance,glutamatergic synapse,Wnt signaling pathway,mTOR signaling pathway,VEGF signaling pathway,etc.Among the five microRNAs,has-miR-26b,has-miR-1972,has-miR-4498 might have more important regulatory functions.Conclusion Bioinformatic analysis indicates that has-miR-26b,has-miR-1972,has-miR-4485,has-miR-4498,and has-miR-4743 are closely related to the mechanism and pathogenesis of major depressive disorder.