CAJ | 학술논문

本研究对前期从云南蚊虫获得的病毒分离物作进一步鉴定，以明确其分类地位。采用甲病毒通用引物进行RT－PCR检测，在5株病毒分离物扩增出目的基因片段。新分离株4株来自三带喙库蚊，1株来自中华按蚊。它们可使白蚊伊蚊细胞（C6／36）、猴肾细胞（Vero）及金黄地鼠肾细胞（BHK－21）发生规律性细胞病变。核苷酸序列分析显示5株病毒与盖塔病毒（ Getah virus， GETV）同源性最高，与GETV中国云南YN0540株、河北株 HB0234、海南株 M1及南韩株、 LEIV MPR 株、鹭山病毒的同源性为96．4％～99．2％，5株病毒之间的同源性为98．4％～100％。系统进化分析结果显示，新分离株与上述GETV处于同一进化分支。以上结果证实5株病毒分离物为盖塔病毒。
본연구대전기종운남문충획득적병독분리물작진일보감정，이명학기분류지위。채용갑병독통용인물진행RT－PCR검측，재5주병독분리물확증출목적기인편단。신분리주4주래자삼대훼고문，1주래자중화안문。타문가사백문이문세포（C6／36）、후신세포（Vero）급금황지서신세포（BHK－21）발생규률성세포병변。핵감산서렬분석현시5주병독여개탑병독（ Getah virus， GETV）동원성최고，여GETV중국운남YN0540주、하북주 HB0234、해남주 M1급남한주、 LEIV MPR 주、로산병독적동원성위96．4％～99．2％，5주병독지간적동원성위98．4％～100％。계통진화분석결과현시，신분리주여상술GETV처우동일진화분지。이상결과증실5주병독분리물위개탑병독。
The aim of this study was to identify viral isolates obtained from mosquitoes in Yunnan Province of China.The newly obtained isolates were identified by RT-PCR using alphavirus universal primers.Four of five isolates were from Culex tritaeniorhynchus and the rest was from Anopheles sinensis.They induced obvious cytopathic effect on C6/36, Vero and BHK-21 cell lines, respectively.Nucleotide sequence analyses showed that five isolates had high homology with Getah virus (GETV) YN0540, HB0234, M1 strains of China, and South Korea, LEIV 17741 MPR strains, and Sagiyama virus, with 96.4%-99.2% identities.The nucleotide sequence identifies among the five new isolates were 98.4%-100%.Phylogenetic analysis showed that the new isolates were clustered with the GETVs.These results confirmed that the new isolates belonged to GETV.