中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2015年
6期
404-407
,共4页
胃肿瘤%聚合酶链反应%钠氢交换蛋白
胃腫瘤%聚閤酶鏈反應%鈉氫交換蛋白
위종류%취합매련반응%납경교환단백
Stomach neoplasms%Polymerase chain reaction%Sodium-hydrogen exchanger
目的 探讨胃癌组织中钠氢交换蛋白(NHE)1的表达水平及其与临床特征的关系.方法 分别应用RT-PCR和Western blot方法检测48例胃癌组织及40例癌旁胃黏膜组织中NHE1mRNA及蛋白的表达情况,并进一步分析其表达与临床病理资料的关系.结果 胃癌组织中NHE1mRNA及蛋白相对表达量分别为0.791±0.286和1.475±0.142,癌旁组织分别为0.352±0.069和0.329±0.048,两者比较差异均有统计学意义(P＜0.01).胃癌组织中NHE1mRNA表达和NHE1蛋白表达显著相关(r=0.375,P＜ 0.05).NHE1 mRNA和蛋白表达与肿瘤浸润深度、淋巴结转移及TNM分期有关(P＜0.05),而与年龄、性别及分化程度无关(P＞0.05).结论 胃癌组织中的NtHE1 mRNA及蛋白的表达水平均显著升高,可能参与了胃癌的发生、发展进程.
目的 探討胃癌組織中鈉氫交換蛋白(NHE)1的錶達水平及其與臨床特徵的關繫.方法 分彆應用RT-PCR和Western blot方法檢測48例胃癌組織及40例癌徬胃黏膜組織中NHE1mRNA及蛋白的錶達情況,併進一步分析其錶達與臨床病理資料的關繫.結果 胃癌組織中NHE1mRNA及蛋白相對錶達量分彆為0.791±0.286和1.475±0.142,癌徬組織分彆為0.352±0.069和0.329±0.048,兩者比較差異均有統計學意義(P＜0.01).胃癌組織中NHE1mRNA錶達和NHE1蛋白錶達顯著相關(r=0.375,P＜ 0.05).NHE1 mRNA和蛋白錶達與腫瘤浸潤深度、淋巴結轉移及TNM分期有關(P＜0.05),而與年齡、性彆及分化程度無關(P＞0.05).結論 胃癌組織中的NtHE1 mRNA及蛋白的錶達水平均顯著升高,可能參與瞭胃癌的髮生、髮展進程.
목적 탐토위암조직중납경교환단백(NHE)1적표체수평급기여림상특정적관계.방법 분별응용RT-PCR화Western blot방법검측48례위암조직급40례암방위점막조직중NHE1mRNA급단백적표체정황,병진일보분석기표체여림상병리자료적관계.결과 위암조직중NHE1mRNA급단백상대표체량분별위0.791±0.286화1.475±0.142,암방조직분별위0.352±0.069화0.329±0.048,량자비교차이균유통계학의의(P＜0.01).위암조직중NHE1mRNA표체화NHE1단백표체현저상관(r=0.375,P＜ 0.05).NHE1 mRNA화단백표체여종류침윤심도、림파결전이급TNM분기유관(P＜0.05),이여년령、성별급분화정도무관(P＞0.05).결론 위암조직중적NtHE1 mRNA급단백적표체수평균현저승고,가능삼여료위암적발생、발전진정.
Objective To determine the expression of sodium-hydrogen exchanger (NHE) 1 in gastric carcinoma tissue,and investigate the relationship between NHE1 expression and clinicopathological characteristics.Methods The expression levels of NHE1 mRNA and protein were detected in both gastric carcinoma tissue (48 cases) and paraneoplastic tissue (40 cases) by reverse transcription polymerase chain reaction (RT-PCR) and Western blot.The relationship between the expression levels of NHE1 mRNA and protein and the clinicopathological characteristics was analyzed.Results The relative expression levels of NHE1 mRNA and protein in gastric carcinoma tissue were 0.791 ± 0.286 and 1.475 ± 0.142,in paraneoplastic tissue were 0.352 ± 0.069 and 0.329 ± 0.048,and there were statistical differences (P ＜ 0.01).The expression level of NHE1 mRNA was positively correlated with NHE 1 protein in the gastric carcinoma tissue (r =0.375,P ＜ 0.05).The expression levels of NHE1 mRNA and protein were associated with the depth of invasion,lymph node metastasis and TNM staging (P ＜ 0.05).However,the expression levels of NHE1 mRNA and protein were no associated with age,gender and tumor differentiation (P ＞ 0.05).Conclusion The expression levels of NHE1 mRNA and protein are significantly up-regulated in gastric carcinoma tissue,which may be involved in the development of gastric carcinoma.
