脑与神经疾病杂志
腦與神經疾病雜誌
뇌여신경질병잡지
JOURNAL OF BRAIN AND NERVOUS DISEASES
2015年
3期
165-169
,共5页
刘鹏%王瑾%苗常青%刘玥%唐鹏%侯辰%张欣%种莉%李晓青%李锐
劉鵬%王瑾%苗常青%劉玥%唐鵬%侯辰%張訢%種莉%李曉青%李銳
류붕%왕근%묘상청%류모%당붕%후신%장흔%충리%리효청%리예
丹参乙酸镁%脑缺血损伤%神经生长因子%脑源性神经营养因子
丹參乙痠鎂%腦缺血損傷%神經生長因子%腦源性神經營養因子
단삼을산미%뇌결혈손상%신경생장인자%뇌원성신경영양인자
Magnesium lithospermate B%Cerebral ischemia injury%Nerve growth factor%Brain-derived neurotrophic factor
目的：观察丹参乙酸镁对局灶性脑缺血后大鼠神经行为、梗死体积、组织形态及缺血半暗带神经生长因子（NGF）、脑源性神经营养因子（BDNF）表达的影响，探讨丹参乙酸镁的神经保护作用及其机制。方法健康雄性SD大鼠36只随机分为3组，Ⅰ组（假手术＋安慰剂组）；Ⅱ组（脑缺血＋安慰剂组）；Ⅲ组（脑缺血＋药物组）。用线栓法建立动物模型，不给予再灌注，各组在术后48h断头取脑，处死前行神经功能评分，用氯化三苯基四氮唑（ TTC）染色计算脑梗死体积，用苏木精－伊红（ HE）染色观察组织学形态，用免疫组织化学染色观察NGF、BDNF的表达。结果Ⅰ组在神经功能评分、梗死体积、组织形态及大脑皮质相应部位NGF、BDNF阳性细胞数均正常。与Ⅱ组相比，Ⅲ组的神经功能评分和梗死体积比较差异无统计学意义（ P＞0．05）；光镜下，Ⅱ组、Ⅲ组缺血性病理改变均较重，两组之间无明显差别；Ⅲ组缺血半暗带NGF、BDNF阳性神经元数较Ⅱ组增加（P＜0．05）。与Ⅰ组相比，Ⅱ组缺血半暗带NGF、BDNF阳性神经元数增加（P＜0．05）。结论丹参乙酸镁可增加脑缺血损伤后缺血半暗带NGF、BDNF的表达，但其发挥的神经保护作用并不明显。
目的：觀察丹參乙痠鎂對跼竈性腦缺血後大鼠神經行為、梗死體積、組織形態及缺血半暗帶神經生長因子（NGF）、腦源性神經營養因子（BDNF）錶達的影響，探討丹參乙痠鎂的神經保護作用及其機製。方法健康雄性SD大鼠36隻隨機分為3組，Ⅰ組（假手術＋安慰劑組）；Ⅱ組（腦缺血＋安慰劑組）；Ⅲ組（腦缺血＋藥物組）。用線栓法建立動物模型，不給予再灌註，各組在術後48h斷頭取腦，處死前行神經功能評分，用氯化三苯基四氮唑（ TTC）染色計算腦梗死體積，用囌木精－伊紅（ HE）染色觀察組織學形態，用免疫組織化學染色觀察NGF、BDNF的錶達。結果Ⅰ組在神經功能評分、梗死體積、組織形態及大腦皮質相應部位NGF、BDNF暘性細胞數均正常。與Ⅱ組相比，Ⅲ組的神經功能評分和梗死體積比較差異無統計學意義（ P＞0．05）；光鏡下，Ⅱ組、Ⅲ組缺血性病理改變均較重，兩組之間無明顯差彆；Ⅲ組缺血半暗帶NGF、BDNF暘性神經元數較Ⅱ組增加（P＜0．05）。與Ⅰ組相比，Ⅱ組缺血半暗帶NGF、BDNF暘性神經元數增加（P＜0．05）。結論丹參乙痠鎂可增加腦缺血損傷後缺血半暗帶NGF、BDNF的錶達，但其髮揮的神經保護作用併不明顯。
목적：관찰단삼을산미대국조성뇌결혈후대서신경행위、경사체적、조직형태급결혈반암대신경생장인자（NGF）、뇌원성신경영양인자（BDNF）표체적영향，탐토단삼을산미적신경보호작용급기궤제。방법건강웅성SD대서36지수궤분위3조，Ⅰ조（가수술＋안위제조）；Ⅱ조（뇌결혈＋안위제조）；Ⅲ조（뇌결혈＋약물조）。용선전법건립동물모형，불급여재관주，각조재술후48h단두취뇌，처사전행신경공능평분，용록화삼분기사담서（ TTC）염색계산뇌경사체적，용소목정－이홍（ HE）염색관찰조직학형태，용면역조직화학염색관찰NGF、BDNF적표체。결과Ⅰ조재신경공능평분、경사체적、조직형태급대뇌피질상응부위NGF、BDNF양성세포수균정상。여Ⅱ조상비，Ⅲ조적신경공능평분화경사체적비교차이무통계학의의（ P＞0．05）；광경하，Ⅱ조、Ⅲ조결혈성병리개변균교중，량조지간무명현차별；Ⅲ조결혈반암대NGF、BDNF양성신경원수교Ⅱ조증가（P＜0．05）。여Ⅰ조상비，Ⅱ조결혈반암대NGF、BDNF양성신경원수증가（P＜0．05）。결론단삼을산미가증가뇌결혈손상후결혈반암대NGF、BDNF적표체，단기발휘적신경보호작용병불명현。
ObjecitveToobservetheneuroprotectiveeffectsofmagnesiumlithospermateBonaratmodelof focal middle cerebral artery occlusion ( MCAO ) .Methods Thirty-six male Sprague-Dawley rats were randomly divided into three groups , groupⅠ( sham operated rats treated with placebo ) , groupⅡ( MCAO ischemia treated with placebo ) and group Ⅲ( MCAO ischemia with magnesium lithospermate B ) .The neuroethology assessment was evaluated at 48h before the rats were executed and don't give reperfusion.The volume of cerebral infarction and the pathologic changes were observed by TTC staining and HE staining .The expression of NGF、BDNF was tested by immunohistochemistry .Results The scores of neurologic deficit and the infarct volumes in group Ⅲ were no significant difference than those in group Ⅱ( P>0.05) .Under optical microscope ,both Ⅱand Ⅲhave more degree of cerebral ischemic injury in pathomorphology ,and there were no significant difference between them .The numbers of NGF and BDNF immunopositive neurons in ischemic penumbra of group Ⅲ were higher than those in group Ⅱ(P<0.05).The numbers of NGF and BDNF immunopositive neurons in ischemic penumbra of group Ⅱ were higher than those in cerebral cortex ofⅠ ( P<0.05 ) .Conclusion Although magnesium lithospermate B can increased NGF、BDNF expression in the brain ischemic penumbra , its effect of neuroprotective against focal cerebral ischemia injury was not unconspicuous .
