CAJ | 학술논문

为了解黑龙江口岸地区斑点热群立克次体在人群、鼠类、蜱类中的分布特征，针对黑龙江省9个中俄边境口岸，采集出入境人群的血液样本215份、鼠类样本204份、蜱类样本188份，利用半巢式PCR方法对所有样本进行斑点热群立克次体特异性片段扩增，通过基因测序对斑点热群立克次体分型。结果在哈尔滨机场及嘉荫两个口岸的大仓鼠及小家鼠中检测到2例劳氏立克次体Rickettsia raoultii，为首次在黑龙江口岸鼠样本中检测出劳氏立克次体；在188份蜱样本中，检出斑点热群立克次体阳性样本101例，感染率达53．7％，其中劳氏立克次体89例，新塔拉塞维奇立克次体Candidatus Rickettsia tarasevichiae 11例，黑龙江立克次体Rickettsia heilongjiangii 1例，同样首次在黑龙江口岸全沟硬蜱中检测到新塔拉塞维奇立克次体。黑龙江中俄边境口岸开展SFGR的长期监测具有非常重要的公共卫生学意义。
위료해흑룡강구안지구반점열군립극차체재인군、서류、비류중적분포특정，침대흑룡강성9개중아변경구안，채집출입경인군적혈액양본215빈、서류양본204빈、비류양본188빈，이용반소식PCR방법대소유양본진행반점열군립극차체특이성편단확증，통과기인측서대반점열군립극차체분형。결과재합이빈궤장급가음량개구안적대창서급소가서중검측도2례로씨립극차체Rickettsia raoultii，위수차재흑룡강구안서양본중검측출로씨립극차체；재188빈비양본중，검출반점열군립극차체양성양본101례，감염솔체53．7％，기중로씨립극차체89례，신탑랍새유기립극차체Candidatus Rickettsia tarasevichiae 11례，흑룡강립극차체Rickettsia heilongjiangii 1례，동양수차재흑룡강구안전구경비중검측도신탑랍새유기립극차체。흑룡강중아변경구안개전SFGR적장기감측구유비상중요적공공위생학의의。
To understand the distribution characteristics of spotted fever group Rickettsia in the residencies, rodents and ticks in Heilongjiang ports, total 9 port area along with Sino-Russian border were sampled to test the species and infection rates of spotted fever group Rickettsia from 188 ticks and 204 rodents.Moreover, 215 humans entry or exit from these ports were also include for screening the possible infection the rickettsia organisms under their permissions.Results from semi-nested PCR and sequences indicated that two Rickettsia raoultii detected in Cricetulus triton and Mus musculus Linnaeus respectively.None rickettsia organism were found in the human samples.While in ticks, 90 R.raoultii, 11 Candidatus Rickettsia tarasevichiae and 1 R.heilongiangii were found.The preliminary suggested the risk of emerged spotted fever pathogens in these areas and the necessary and urgent of long term monitoring on tick borne diseases.