CAJ | 학술논문

运用蛋白质组学相关技术对暴露于酸化海水中的中华哲水蚤全蛋白进行分析，结果表明，对照组、CCO 20．08％和 CCO20．20％处理组中华哲水蚤的双向电泳图谱上可以分别分辨出1191、1117和946个蛋白点，选取其中43个差异蛋白进行 MALDI－TOF／TOF 质谱鉴定，成功鉴定出23个差异蛋白，这些蛋白主要与蛋白合成和分解、能量代谢、DNA 分子修复以及解毒过程有关。
운용단백질조학상관기술대폭로우산화해수중적중화철수조전단백진행분석，결과표명，대조조、CCO 20．08％화 CCO20．20％처리조중화철수조적쌍향전영도보상가이분별분변출1191、1117화946개단백점，선취기중43개차이단백진행 MALDI－TOF／TOF 질보감정，성공감정출23개차이단백，저사단백주요여단백합성화분해、능량대사、DNA 분자수복이급해독과정유관。
This study compared proteim profiles of Calanus sinicus cultured under CCO 2 0.08% and CCO2 0.20%CO2-acidified seawater for 4 days using a proteomic approach,and identified the differentially expressed proteins. The results are shown that,1 191,1 117 and 946 protein spots of C.sinicus in control,CCO 2 0.08% and CCO2 0.20%groups were detected in the two-dimensional electrophoresis gels,respectively.The 43 protein spots were selected based on their differential expression between control and CCO2 0.08% group,CCO2 0.20% group,and 23 proteins of which were identified by MALDI-TOF/TOF mass spectrometry.The data demonstrated that these differentially expressed proteins were associated with protein synthesize and proteolysis,energy metabolism,DNA repaired and detoxified of organisms.