海洋学报（中文版）
海洋學報（中文版）
해양학보（중문판）
ACTA OCEANOLOGICA SINICA
2015年
6期
114-119
,共6页
丁兰平%颜泽伟%张全亮%徐佩杭%黄冰心
丁蘭平%顏澤偉%張全亮%徐珮杭%黃冰心
정란평%안택위%장전량%서패항%황빙심
丝状体%特定生长率%藻红蛋白%藻蓝蛋白%培养方式%换水频率
絲狀體%特定生長率%藻紅蛋白%藻藍蛋白%培養方式%換水頻率
사상체%특정생장솔%조홍단백%조람단백%배양방식%환수빈솔
conchocelis%SGR%PE%PC%culture methods%frequency of the medium
本实验以坛紫菜自由丝状体为材料，研究不同培养方式（静置、摇床、通气）和换水频率（5 d、7 d、10 d、20 d）对丝状体生长及藻胆蛋白（PE、PC）含量变化的影响。研究结果表明：坛紫菜自由丝状体在摇床培养条件下生长较好，特定生长速率可达10．8327％～10．8915％；摇床培养下的丝状体 PE 含量相对静置培养的较高一些，通气培养的相对较低，最高为36．8168 mg／g；静置条件下培养的丝状体PC 含量最高，摇床培养次之，通气培养最低，最低为1．0824 mg／g。相同培养方式下，换水频率对丝状体特定生长速率影响不大；除通气培养外，换水频率20 d 其 PE、PC 含量较高。方差分析结果表明：实验范围内的培养方式及换水频率对丝状体特定生长速率大小、PE 和 PC 含量不存在交互作用。本文实验结果对坛紫菜自由丝状体产业化应用于食品加工、色素提取等产业提供了实验理论和依据。
本實驗以罈紫菜自由絲狀體為材料，研究不同培養方式（靜置、搖床、通氣）和換水頻率（5 d、7 d、10 d、20 d）對絲狀體生長及藻膽蛋白（PE、PC）含量變化的影響。研究結果錶明：罈紫菜自由絲狀體在搖床培養條件下生長較好，特定生長速率可達10．8327％～10．8915％；搖床培養下的絲狀體 PE 含量相對靜置培養的較高一些，通氣培養的相對較低，最高為36．8168 mg／g；靜置條件下培養的絲狀體PC 含量最高，搖床培養次之，通氣培養最低，最低為1．0824 mg／g。相同培養方式下，換水頻率對絲狀體特定生長速率影響不大；除通氣培養外，換水頻率20 d 其 PE、PC 含量較高。方差分析結果錶明：實驗範圍內的培養方式及換水頻率對絲狀體特定生長速率大小、PE 和 PC 含量不存在交互作用。本文實驗結果對罈紫菜自由絲狀體產業化應用于食品加工、色素提取等產業提供瞭實驗理論和依據。
본실험이단자채자유사상체위재료，연구불동배양방식（정치、요상、통기）화환수빈솔（5 d、7 d、10 d、20 d）대사상체생장급조담단백（PE、PC）함량변화적영향。연구결과표명：단자채자유사상체재요상배양조건하생장교호，특정생장속솔가체10．8327％～10．8915％；요상배양하적사상체 PE 함량상대정치배양적교고일사，통기배양적상대교저，최고위36．8168 mg／g；정치조건하배양적사상체PC 함량최고，요상배양차지，통기배양최저，최저위1．0824 mg／g。상동배양방식하，환수빈솔대사상체특정생장속솔영향불대；제통기배양외，환수빈솔20 d 기 PE、PC 함량교고。방차분석결과표명：실험범위내적배양방식급환수빈솔대사상체특정생장속솔대소、PE 화 PC 함량불존재교호작용。본문실험결과대단자채자유사상체산업화응용우식품가공、색소제취등산업제공료실험이론화의거。
The cultivation methods (standing,rocking,ventilating)and the frequency in changing water (once in 5 d,7 d,10 d,20 d )were studied on influence of the growth and phycobiliprotein (PE,PC)content of free living Conchocelis of Porphyra haitanensis .The results show that under the condition of rocking,its growth is better, which SGR can reach 10.832 7%-10.891 5%;and its PE content is higher than that of under the condition of standing but lower than that of under the condition of ventilating,and the highest value is 36.816 8 mg/g.Under standing,the PC content reaches the highest,which higher than that of under rocking,and it reaches the lowest under ventilating with value 1.082 4 mg/g.Under the same culture way,the frequency in changing water does not much affect the SGR of free living Conchocelis.However,when the frequency is once in 20 d,its PE and PC con-tents are higher than other frequency except those of under the condition of ventilating.The ANOVA results show that interaction between the culture methods and water changing frequency does not exist on the SGR,PE and PC content of the conchocelis.It provides an experimental and theoretical basis for free living Conchocelis of Porphyra haitanensis applying to industrial application in food processing and pigment picking industry.
