CAJ | 학술논문

为克服静力称重法受蒸发量影响大的不足，同时适应在线测量的需要，基于 Lambert-Beer 定律，研究了520 nm 和730 nm 双波长超微液体容量光度吸收测量法，通过稀溶液浓度与吸光度之间的线性关系可以计算出待测超微量液体容积。设计了双波长参比光度吸收法的测量系统，采用与静力称重测量系统比对试验的方式对这种方法进行了验证，1μL 和5μL 微量液体试验数据表明：双波长参比光度吸收法和静力称重法测量结果具有良好的一致性，均符合 ISO 8655的技术要求。与静力称重法方法相比，双波长参比光度吸收法对测量环境要求低，易于实现在线测量，而且可以有效减少液体蒸发对测量结果的影响。
위극복정력칭중법수증발량영향대적불족，동시괄응재선측량적수요，기우 Lambert-Beer 정률，연구료520 nm 화730 nm 쌍파장초미액체용량광도흡수측량법，통과희용액농도여흡광도지간적선성관계가이계산출대측초미량액체용적。설계료쌍파장삼비광도흡수법적측량계통，채용여정력칭중측량계통비대시험적방식대저충방법진행료험증，1μL 화5μL 미량액체시험수거표명：쌍파장삼비광도흡수법화정력칭중법측량결과구유량호적일치성，균부합 ISO 8655적기술요구。여정력칭중법방법상비，쌍파장삼비광도흡수법대측량배경요구저，역우실현재선측량，이차가이유효감소액체증발대측량결과적영향。
In order to overcome the shortcoming of significant influence of evaporation capacity on hydro-weighting method,a dual-wavelength photometric method based on Lambert-Beer law at 520 nm and 730 nm was introduced. Micro liquid volume to be measured can be calculated through the linear relation between dilute solution concentration and absorbance. Comparing with the gravimetric method,experimental systems for dual-wavelength photometric method is designed. The test data at 1 μL and 5 μL indicates that dual-wavelength photometric method is in good conformance with gravimetric method,and meet the technical requirement of ISO 8655,but the measured value of dual-wavelength photometric method is a little bigger. dual-wavelength photometric method is able to provide a good solution for micro liquid volume measurement. By comparing with gravimetric method,dual-wavelength photometric method is low in requirement of measurement environment,and easy to realize the online calibration and capable of effectively reducing the influence of liquid evaporation.