CAJ | 학술논문

目的：建立高效液相色谱法测定注射用头孢他啶聚合物的含量。方法采用高效液相色谱法,色谱柱为TSK-GEL G2000SWXL 凝胶色谱柱,流动相为磷酸盐缓冲液(pH7.0)0.005 mol·L-1磷酸氢二钠溶液-0.005 mol·L-1磷酸二氢钠溶液(61：39),检测波长231 nm,柱温30℃,进样量20μL 。结果头孢他啶对照品检测线性范围为0.51~25.64μg·mL-1(r=0.999),注射用头孢他啶聚合物检测线性范围为0.20~3.92 mg·mL-1(r =0.99),聚合物定量限为0.71μg。结论该方法快速,分离度好,可用于注射用头孢他啶聚合物的检测。
목적：건립고효액상색보법측정주사용두포타정취합물적함량。방법채용고효액상색보법,색보주위TSK-GEL G2000SWXL 응효색보주,류동상위린산염완충액(pH7.0)0.005 mol·L-1린산경이납용액-0.005 mol·L-1린산이경납용액(61：39),검측파장231 nm,주온30℃,진양량20μL 。결과두포타정대조품검측선성범위위0.51~25.64μg·mL-1(r=0.999),주사용두포타정취합물검측선성범위위0.20~3.92 mg·mL-1(r =0.99),취합물정량한위0.71μg。결론해방법쾌속,분리도호,가용우주사용두포타정취합물적검측。
Objective To establish an HPLC method for the determination of ceftazidime for injection. Methods HPLC column was TSK-GEL G2000SWXL gel column. Mobile phase of phosphate buffer ( pH 7. 0) was 0. 005 mol · L-1 disodium hydrogen phosphate solution-0. 005 mol · L-1 sodium dihydrogen phosphate solution ( 61 : 39 ). The detection wavelength was 231 nm; column temperature was 30 ℃ ; the injection volume was 20 μL. Results Ceftazidime reference linear range was 0. 51-25. 64 μg·mL-1(r=0. 999), ceftazidime for injection polymer linear range was 0. 20-3. 92 mg·mL-1 (r=0. 99), and the limit of quantification polymer was 0. 71 μg. Conclusion The method is rapid and the separation was good. It can be used for the detection of ceftazidime for injection.