CAJ | 학술논문

目的：检测壳聚糖凝胶对醋酸诱导的大鼠直肠损伤的影响。方法将大鼠麻醉,以4%醋酸棉签经大鼠肛门置入直肠1 min,深度3 cm,制备大鼠直肠损伤模型36只,随机分为模型对照组、阳性对照组和药物组,每组12只。药物组经肛门给予壳聚糖凝胶1575 mg·kg-1,阳性对照组给予痔疮膏1575 mg·kg-1,连续6 d,给药后30 min,1,4,6 d,各组分别处死大鼠3只,解剖大鼠,肉眼观察大鼠直肠损伤情况,观察各组之间在给药各时间段的直肠病理学形态差异。结果给药组用药30 min,1,4,6 d 后,大鼠直肠黏膜损伤计分较模型对照组和阳性对照组低,上皮组织坏死、间质充血、出血、水肿、腺体破坏、炎症细胞浸润等病理现象明显较模型对照组和阳性对照组轻,修复速度较模型对照组和阳性对照组快,并且没有较明显的瘢痕出现。结论壳聚糖凝胶可明显减轻4%醋酸造成的直肠损伤,具有一定的治疗作用。
목적：검측각취당응효대작산유도적대서직장손상적영향。방법장대서마취,이4%작산면첨경대서항문치입직장1 min,심도3 cm,제비대서직장손상모형36지,수궤분위모형대조조、양성대조조화약물조,매조12지。약물조경항문급여각취당응효1575 mg·kg-1,양성대조조급여치창고1575 mg·kg-1,련속6 d,급약후30 min,1,4,6 d,각조분별처사대서3지,해부대서,육안관찰대서직장손상정황,관찰각조지간재급약각시간단적직장병이학형태차이。결과급약조용약30 min,1,4,6 d 후,대서직장점막손상계분교모형대조조화양성대조조저,상피조직배사、간질충혈、출혈、수종、선체파배、염증세포침윤등병리현상명현교모형대조조화양성대조조경,수복속도교모형대조조화양성대조조쾌,병차몰유교명현적반흔출현。결론각취당응효가명현감경4%작산조성적직장손상,구유일정적치료작용。
Objective To detect the effect of chitosan gel on rectal injury which was induced by acetic acid. Methods 36 rats were anesthetized, 4% acetic acid swab was placed into the rectum (3 cm in depth for 1 min) to establish rectum injury model. These rats were divided into model control group, positive control group and drug group with 12 rats each group randomly. Chitosan gel was given to the drug group through anus with the dose of 1 575 mg·kg-1 , and hemorrhoid cream (1 575 mg·kg-1 ) was administered in the positive control group for 6 successive days. At 30 min, 1, 4, 6 day( s) after administration, 3 rats of each group were sacrificed, and were dissected, rectal injury and rectal pathology morphology changing of each group at different time points of administration were compared through visual observation. Results At 30 min, 1, 4, and 6 day(s) after administrating chitosan gel, score of rectum mucosa injury in dury group was lower than those in model control and positive control group. Pathological changes ( including epithelial tissue necrosis, mesenchyme hyperemia, hemorrhage, edema, gland injury, infiltration of inflammatory cells) were significantly slighter in drug group than in model control group and positive control group. Those lesions were restored faster in drug group than in model control group and positive control group, and there was no obvious scar. Conclusion Chitosan gel can obviously alleviate the rectal injury caused by 4% acetic acid and has therapeutic effect.