医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2015年
6期
726-729
,共4页
李美波%顾生玖%赵文鹏%朱开梅
李美波%顧生玖%趙文鵬%硃開梅
리미파%고생구%조문붕%주개매
紫杉醇%脂质体,热敏,长循环%癌,肺
紫杉醇%脂質體,熱敏,長循環%癌,肺
자삼순%지질체,열민,장순배%암,폐
Paclitaxel%Liposome,thermo-sensitive,long-circulating%Cancer,lung
目的：研究紫杉醇长循环热敏脂质体(PLTL)对 Lewis 肺癌移植瘤的抑制作用。方法建立小鼠 Lewis 肺癌模型,将40只荷瘤鼠随机分为5组,空白对照组、模型对照组、紫杉醇注射液(PTX)组、紫杉醇热敏脂质体(PTL)组和PLTL 组,每组8只。空白对照组和模型对照组注射0.9%氯化钠溶液,PTX 组、PTL 组和 PLTL 组,每只小鼠给药总剂量为紫杉醇质量浓度为20 mg·kg-1,采用0.9%氯化钠溶液稀释,每次注射0.2 mL。除空白对照组外,其他组给药后5 min内立即将小鼠的肿瘤部位固定于(42±0.5)℃的恒温水浴中加热30 min。治疗期间观察移植瘤体生长情况,苏木精-伊红(HE)染色检测肿瘤组织的病理学形态改变,观察肿瘤细胞的形态学变化,采用流式细胞术检测肿瘤细胞的凋亡率,考察PLTL 联合局部热疗对小鼠肿瘤的抑瘤作用。结果模型对照组、PTX 组、PTL 组和 PLTL 组抑瘤率分别为21.81%,48.87%,57.22%和78.87%,肿瘤细胞凋亡率分别为(20.4±4.2)%,(42.7±3.8)%,(54.6±2.9)%和(69.7±5.0)%,肿瘤组织切片 HE 染色发现,PLTL 组肿瘤细胞凋亡与坏死数量明显增多。结论 PLTL 与 PTX,PTL 比较,具有明显的热敏靶向性,与局部热疗相结合显著提高紫杉醇的治疗效果。
目的：研究紫杉醇長循環熱敏脂質體(PLTL)對 Lewis 肺癌移植瘤的抑製作用。方法建立小鼠 Lewis 肺癌模型,將40隻荷瘤鼠隨機分為5組,空白對照組、模型對照組、紫杉醇註射液(PTX)組、紫杉醇熱敏脂質體(PTL)組和PLTL 組,每組8隻。空白對照組和模型對照組註射0.9%氯化鈉溶液,PTX 組、PTL 組和 PLTL 組,每隻小鼠給藥總劑量為紫杉醇質量濃度為20 mg·kg-1,採用0.9%氯化鈉溶液稀釋,每次註射0.2 mL。除空白對照組外,其他組給藥後5 min內立即將小鼠的腫瘤部位固定于(42±0.5)℃的恆溫水浴中加熱30 min。治療期間觀察移植瘤體生長情況,囌木精-伊紅(HE)染色檢測腫瘤組織的病理學形態改變,觀察腫瘤細胞的形態學變化,採用流式細胞術檢測腫瘤細胞的凋亡率,攷察PLTL 聯閤跼部熱療對小鼠腫瘤的抑瘤作用。結果模型對照組、PTX 組、PTL 組和 PLTL 組抑瘤率分彆為21.81%,48.87%,57.22%和78.87%,腫瘤細胞凋亡率分彆為(20.4±4.2)%,(42.7±3.8)%,(54.6±2.9)%和(69.7±5.0)%,腫瘤組織切片 HE 染色髮現,PLTL 組腫瘤細胞凋亡與壞死數量明顯增多。結論 PLTL 與 PTX,PTL 比較,具有明顯的熱敏靶嚮性,與跼部熱療相結閤顯著提高紫杉醇的治療效果。
목적：연구자삼순장순배열민지질체(PLTL)대 Lewis 폐암이식류적억제작용。방법건립소서 Lewis 폐암모형,장40지하류서수궤분위5조,공백대조조、모형대조조、자삼순주사액(PTX)조、자삼순열민지질체(PTL)조화PLTL 조,매조8지。공백대조조화모형대조조주사0.9%록화납용액,PTX 조、PTL 조화 PLTL 조,매지소서급약총제량위자삼순질량농도위20 mg·kg-1,채용0.9%록화납용액희석,매차주사0.2 mL。제공백대조조외,기타조급약후5 min내립즉장소서적종류부위고정우(42±0.5)℃적항온수욕중가열30 min。치료기간관찰이식류체생장정황,소목정-이홍(HE)염색검측종류조직적병이학형태개변,관찰종류세포적형태학변화,채용류식세포술검측종류세포적조망솔,고찰PLTL 연합국부열료대소서종류적억류작용。결과모형대조조、PTX 조、PTL 조화 PLTL 조억류솔분별위21.81%,48.87%,57.22%화78.87%,종류세포조망솔분별위(20.4±4.2)%,(42.7±3.8)%,(54.6±2.9)%화(69.7±5.0)%,종류조직절편 HE 염색발현,PLTL 조종류세포조망여배사수량명현증다。결론 PLTL 여 PTX,PTL 비교,구유명현적열민파향성,여국부열료상결합현저제고자삼순적치료효과。
Objective To observe the effect of paclitaxel long-circulating thermo-sensitive liposome ( PLTL) on inhibiting the growth of transplanting Lewis lung cancer cells in C57BL/ 6 mice. Methods The model of mice carried Lewis lung cancer was established. 40 tumor-bearing mice were divided into five groups randomly: blank control group, model control group, PTX group, PTL group and PLTL group, 8 mice for each group. The blank control group and the model control group were injected with 0. 9% sodium chloride solution. In PTX group, PTL group and PLTL group, the dose of per injection was calculated with the reference of PTX for 20 mg·kg-1 , diluted with 0. 9% sodium chloride solution, and the mice were injected via the tail vein with a volume of 0. 2 mL each time. Except for the blank control group, 5 minutes after administration, the tumors of the other groups were subjected to local hyperthermia at (42±0. 5) ℃ for 30 min. During the treatment period, transplantation tumor growth was observed; pathological morphology changes of tumor tissues and cells were detected by HE stain; apoptosis rate of tumor cells was determined by flow cytometry to investigate the inhibition effect of PLTL combined with local thermal therapy on the tumor. Results The inhibition rate of tumor in model control group, PTX group, PTL group and PLTL group was 21. 81% , 48. 87% , 57. 22% and 78. 87% , respectively. The apoptosis rate of tumor cells was (20. 4 ± 4. 2)% , (42. 7 ± 3. 8)% , (54. 6±2. 9)% and (69. 7±5. 0)% , respectively. Observed by pathology, apoptosis rate and necrosis number of tumor cells in PLTL group were significantly increased. Conclusion Compared with PTX and PTL, PLTL has an evident thermo-sensitive feature and can increase the anticancer effect of paclitaxel remarkably when combined with local hyperthermia.
